Colocalization of Leptin Receptor (OB-R) mRNA and Placental Lactogen-II in Rat Trophoblast Cells: Gestational Profile of OB-R mRNA Expression in Placentae

Kawai, Motoyuki; Murakami, Takashi; Otani, Shizuka; Shima, Kenji; Yamaguchi, Masatoshi; Kishi, Kurajiro

doi:10.1006/bbrc.1999.0467

Cited by 13 publications

(8 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Although all tissue samples were collected after the luteal‐placental shift, we report that long and short isoforms of the leptin receptor are constitutively expressed in placenta, as well as in two distinct adipose depots, throughout most of baboon pregnancy. This finding contrasts with a recent report, which documented an increase in placental leptin receptor mRNA transcripts with advancing gestation in the rat (20). However, certain differences may be inherent between primate and rat pregnancy.…”

Section: Discussioncontrasting

confidence: 99%

Leptin Receptor Transcripts Are Constitutively Expressed in Placenta and Adipose Tissue with Advancing Baboon Pregnancy

Green

O'neil

Swan³

et al. 2000

Proc Soc Exp Biol Med

View full text Add to dashboard Cite

Abstract. The baboon (Papio sp.) is an accepted nonhuman primate model for the study of the endocrinology of human pregnancy. To further characterize this model with regard to leptin function, messenger RNA transcripts for both long (Ob‐RL) and short (Ob‐RS) leptin receptor isoforms were identified in maternal tissues at various stages of gestation. Thus, placental villous, subcutaneous and omental adipose tissues were collected upon cesarean delivery at early (Days 60–62), mid (Days 98–102) and late (Days 159–164) pregnancy (term ≈ 184 days). Additionally, amniochorion, decidua, and corpus luteum were collected in late gestation. Expression of Ob‐RL and Ob‐RS transcripts was determined in relation to constitutively expressed glyceraldehyde‐3‐phosphate dehydrogenase via reverse transcriptase‐polymerase chain reaction, and transcripts were localized within specific placental cell types by in situ hybridization. Ob‐RL and Ob‐RS transcripts were present in amniochorion, decidua, and corpus luteum at term and appeared constitutively expressed throughout gestation in placenta and adipose tissues. Ob‐RS was expressed in greater (P < 0.02) abundance than Ob‐RL in all tissues. Within the placenta, receptor isoforms were localized predominantly to the syncytiotrophoblast. The expression of leptin receptor transcripts in maternal adipose tissues, as well as in the syncytiotrophoblast, amniochorion, decidua, and corpus luteum, suggests the potential for autocrine/paracrine roles for the polypeptide in the endocrinology of primate pregnancy. These are the first such observations in a nonhuman primate and support the use of the baboon as a model for the study of leptin in human pregnancy.

show abstract

Section: Discussioncontrasting

confidence: 99%

Leptin Receptor Transcripts Are Constitutively Expressed in Placenta and Adipose Tissue with Advancing Baboon Pregnancy

Green

O'neil

Swan³

et al. 2000

Proc Soc Exp Biol Med

View full text Add to dashboard Cite

show abstract

“…Moreover, Morash et al (15,16) described leptin receptors (both, ObRb and ObRs) in the rat pituitary. Expression of leptin receptors was also found in the placenta (17,18). These data suggest that leptin may regulate mother's metabolism, fetus growth and development via pituitary, hypothalamic and placental receptors.…”

Section: Introductionmentioning

confidence: 62%

Leptin and its receptors in the course of pregnancy in the rat

Szczepankiewicz¹,

Wojciechowicz²,

Kaczmarek³

et al. 2006

Int J Mol Med

View full text Add to dashboard Cite

Control of processes responsible for food intake and regulation of energy homeostasis during pregnancy is crucial for mother as well as for fetus development. Leptin is one of the main hormonal factors involved in regulation these processes in organisms. During pregnancy leptin regulates mother's energy balance and may also affect fetus growth and development, particularly via receptors in hypothalamus arcuate nuclei (ARC), pituitary and placenta. In the present study, serum leptin levels and expression of both short (ObRs) and long (ObRb) form of leptin receptor in the hypothalamus, pituitary and placenta were measured in the course of pregnancy. The results of these studies indicate that leptin concentration in serum increases during pregnancy and decreases 24 h after the delivery. The expression of both short and long forms of the leptin receptor in the hypothalamus decreases in the course of pregnancy and increases after the delivery. In the pituitary, however, a decrease of leptin receptor mRNA during pregnancy was observed only for ObRb. Analysis of placental leptin receptor expression demonstrated an increase of ObRb and constant high levels of ObRs mRNA. Our results suggest that changes in leptin level and its receptor expression may influence the energy homeostasis during pregnancy. In addition, changes in ObR expression are suggestive for: i) leptin resistance in the hypothalamus and pituitary; and ii) an increased leptin-dependent signaling in the placenta.

show abstract

“…No increase in serum leptin levels was noted with advancing pregnancy, but a decline did occur by Day 20. In a more recent study, Kawai et al [84] colocalized a variety of splice variants of the leptin receptor, as well as placental lactogen-II, in the labyrinth zone of the placenta on Days 19-21. In an earlier study [83], investigators were not able to demonstrate leptin mRNA in either the placenta or decidua.…”

Section: In Rodent Pregnancymentioning

confidence: 99%

Leptin in Pregnancy

Henson

Castracane

2000

Biology of Reproduction

264

198

View full text Add to dashboard Cite

Leptin is a polypeptide hormone that aids in the regulation of body weight and energy homeostasis and is linked to a variety of reproductive processes in both animals and humans. Thus, leptin may help regulate ovarian development and steroidogenesis and serve as either a primary signal initiating puberty or as a permissive regulator of sexual maturation. Perhaps significantly, peripheral leptin concentrations, adjusted for adiposity, are dramatically higher in females than in males throughout life. During primate pregnancy, maternal levels that arise from adipose stores and perhaps the placenta increase with advancing gestational age. Proposed physiological roles for leptin in pregnancy include the regulation of conceptus growth and development, fetal/placental angiogenesis, embryonic hematopoiesis, and hormone biosynthesis within the maternal-fetoplacental unit. The specific localization of both leptin and its receptor in the syncytiotrophoblast implies autocrine and/or paracrine relationships in this endocrinologically active tissue. Interactions of leptin with mechanisms regulating pre-eclampsia and maternal diabetes have also been suggested. Collectively, therefore, reports suggest that a better understanding of the regulation of leptin and its role(s) throughout gestation may eventually impact those causes of human perinatal morbidity and mortality that are exacerbated by intrauterine growth retardation, macrosomia, placental insufficiency, or prematurity.

show abstract

Colocalization of Leptin Receptor (OB-R) mRNA and Placental Lactogen-II in Rat Trophoblast Cells: Gestational Profile of OB-R mRNA Expression in Placentae

Cited by 13 publications

References 32 publications

Leptin Receptor Transcripts Are Constitutively Expressed in Placenta and Adipose Tissue with Advancing Baboon Pregnancy

Leptin Receptor Transcripts Are Constitutively Expressed in Placenta and Adipose Tissue with Advancing Baboon Pregnancy

Leptin and its receptors in the course of pregnancy in the rat

Leptin in Pregnancy

Contact Info

Product

Resources

About