Colocalization of sucrose synthase expression and sucrose storage in the sugarbeet taproot indicates a potential role for sucrose catabolism in sucrose accumulation

Fugate, Karen Klotz; Eide, John D.; Martins, Daniel N.; Grusak, Michael A.; Deckard, Edward L.; Finger, Fernando Luíz

doi:10.1016/j.jplph.2019.153016

Cited by 19 publications

(11 citation statements)

References 60 publications

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“…These results can be explained based on the sink capacity of the roots. Increasing the sink capacity lowers the local concentration of sucrose in sink organs, which makes it possible to unload more sucrose from the source organ into the sink organ across a concentration gradient through the phloem ( Rouhier and Usuda, 2001 ; Mitsui et al, 2015 ; Fugate et al, 2019 ; Stein and Granot, 2019 ). Therefore, the resulting fructose by sucrose degradation could accumulate in the large sink organs.…”

Section: Discussionmentioning

confidence: 99%

“…The initial approach of this study was to identify a specific molecular mechanism that influences the high sucrose content in IT-8 roots. Invertases, SPS, and SPP are responsible for the storage and re-synthesis of sucrose in sugar cane stalk, and SUSYs are involved in the storage of sucrose in sugar beet roots ( Giaquinta, 1979 ; Wang et al, 2013 ; Fugate et al, 2019 ). SUC1 and SUC2 are responsible for apoplastic phloem unloading and the cellular uptake of sucrose into sink cells ( Wang et al, 2016 ; Julius et al, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

“…A positive R score between the sucrose content and the CWINV-encoding gene was achieved when IT-8 was excluded ( Supplementary Figure 6 ). Sucrose transport can also occur via the symplastic pathway via plasmodesmata ( Giaquinta, 1979 ; Fugate et al, 2019 ) and this pathway is considered the most important for the sink organs in plants ( Verbančič et al, 2018 ). Therefore, the symplastic pathway could be partially involved in the high sucrose content of IT-8 roots.…”

Section: Discussionmentioning

confidence: 99%

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Analysis of Phenotypic Characteristics and Sucrose Metabolism in the Roots of Raphanus sativus L.

et al. 2021

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The taproot of radish (Raphanus sativus L.) is an important sink organ; it is morphologically diverse and contains large amounts of secondary metabolites. Sucrose metabolism is believed to be important in the development of sink organs. We measured the amounts of glucose, fructose, and sucrose in the roots of sixty three radish accessions and analyzed the association between the sugar content and the root phenotype. Fructose content correlated with the root color and length characteristics, glucose was the most abundant sugar in the roots, and the sucrose content was very low, compared to that of the hexoses in most of the accessions. Expression analysis of the genes involved in sucrose metabolism, transportation, starch synthesis, and cell wall synthesis was performed through RNA sequencing. The genes encoding sucrose synthases (SUSY) and the enzymes involved in the synthesis of cellulose were highly expressed, indicating that SUSY is involved in cell wall synthesis in radish roots. The positive correlation coefficient (R) between the sucrose content and the expression of cell wall invertase and sugar transporter proteins suggest that hexose accumulation could occur through the apoplastic pathway in radish roots. A positive R score was also obtained when comparing the expression of genes encoding SUSY and fructokinase (FK), suggesting that the fructose produced by SUSY is mostly phosphorylated by FK. In addition, we concluded that sucrose was the most metabolized sugar in radish roots.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Analysis of Phenotypic Characteristics and Sucrose Metabolism in the Roots of Raphanus sativus L.

et al. 2021

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“…The degradation of sucrose was mainly catalyzed by SUS in sink tissues, and SUS activity was an indicator for sink strength [ 67 ]. Recent studies have proved that high expression of SUS could promote sucrose accumulation [ 15 , 68 ]. The higher expression of present SUS arahy.DW5X6C indicated a stronger sink strength in sucrose accumulation in ICG 12625 than in Zhonghua 10.…”

Section: Discussionmentioning

confidence: 99%

Key Regulators of Sucrose Metabolism Identified through Comprehensive Comparative Transcriptome Analysis in Peanuts

Huang

Liu

et al. 2021

IJMS

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Sucrose content is a crucial indicator of quality and flavor in peanut seed, and there is a lack of clarity on the molecular basis of sucrose metabolism in peanut seed. In this context, we performed a comprehensive comparative transcriptome study on the samples collected at seven seed development stages between a high-sucrose content variety (ICG 12625) and a low-sucrose content variety (Zhonghua 10). The transcriptome analysis identified a total of 8334 genes exhibiting significantly different abundances between the high- and low-sucrose varieties. We identified 28 differentially expressed genes (DEGs) involved in sucrose metabolism in peanut and 12 of these encoded sugars will eventually be exported transporters (SWEETs). The remaining 16 genes encoded enzymes, such as cell wall invertase (CWIN), vacuolar invertase (VIN), cytoplasmic invertase (CIN), cytosolic fructose-bisphosphate aldolase (FBA), cytosolic fructose-1,6-bisphosphate phosphatase (FBP), sucrose synthase (SUS), cytosolic phosphoglucose isomerase (PGI), hexokinase (HK), and sucrose-phosphate phosphatase (SPP). The weighted gene co-expression network analysis (WGCNA) identified seven genes encoding key enzymes (CIN, FBA, FBP, HK, and SPP), three SWEET genes, and 90 transcription factors (TFs) showing a high correlation with sucrose content. Furthermore, upon validation, six of these genes were successfully verified as exhibiting higher expression in high-sucrose recombinant inbred lines (RILs). Our study suggested the key roles of the high expression of SWEETs and enzymes in sucrose synthesis making the genotype ICG 12625 sucrose-rich. This study also provided insights into the molecular basis of sucrose metabolism during seed development and facilitated exploring key candidate genes and molecular breeding for sucrose content in peanuts.

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“…The strict regulation of sucrose synthesis, transport and use affects fruit growth and development [ 13 ]. In recent years, sucrose metabolism has been extensively studied in such edible fruit as Prunus persica [ 14 ], Prunus armeniaca [ 15 , 16 ], grapes [ 8 ], apples [ 11 , 17 ], and Sacharum officinarum [ 18 ], plus Allium cepa [ 19 ], Beta vulgaris [ 20 ], and Arachis hypogaea [ 21 ]. Sucrose synthase (SuSy), sucrose phosphate synthase (SPS), neutral invertase (NI), cell wall acid invertase (CWINV), and soluble acid invertase (SAI) significantly affect the synthesis and catabolism of soluble sugars in fruit [ 8 , 22 , 23 ].…”

Section: Introductionmentioning

confidence: 99%

Sugar Metabolism and Transcriptome Analysis Reveal Key Sugar Transporters during Camellia oleifera Fruit Development

Chen

Yang

et al. 2022

IJMS

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Camellia oleifera is a widely planted woody oil crop with economic significance because it does not occupy cultivated land. The sugar-derived acetyl-CoA is the basic building block in fatty acid synthesis and oil synthesis in C. oleifera fruit; however, sugar metabolism in this species is uncharacterized. Herein, the changes in sugar content and metabolic enzyme activity and the transcriptomic changes during C. oleifera fruit development were determined in four developmental stages (CR6: young fruit formation; CR7: expansion; CR9: oil transformation; CR10: ripening). CR7 was the key period of sugar metabolism since it had the highest amount of soluble sugar, sucrose, and glucose with a high expression of genes related to sugar transport (four sucrose transporters (SUTs) or and one SWEET-like gene, also known as a sugar, will eventually be exported transporters) and metabolism. The significant positive correlation between their expression and sucrose content suggests that they may be the key genes responsible for sucrose transport and content maintenance. Significantly differentially expressed genes enriched in the starch and sucrose metabolism pathway were observed in the CR6 versus CR10 stages according to KEGG annotation. The 26 enriched candidate genes related to sucrose metabolism provide a molecular basis for further sugar metabolism studies in C. oleifera fruit.

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Colocalization of sucrose synthase expression and sucrose storage in the sugarbeet taproot indicates a potential role for sucrose catabolism in sucrose accumulation

Cited by 19 publications

References 60 publications

Analysis of Phenotypic Characteristics and Sucrose Metabolism in the Roots of Raphanus sativus L.

Analysis of Phenotypic Characteristics and Sucrose Metabolism in the Roots of Raphanus sativus L.

Key Regulators of Sucrose Metabolism Identified through Comprehensive Comparative Transcriptome Analysis in Peanuts

Sugar Metabolism and Transcriptome Analysis Reveal Key Sugar Transporters during Camellia oleifera Fruit Development

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