Colocalization of the rat homolog of the von Hippel Lindau (Vhl) gene and the plasma membrane Ca⁺⁺ transporting ATPase isoform 2 (<i>Atp2b2</i>) gene to rat chromosome bands 4q41.3→42.1

Abstract

Using fluorescence in situ hybridization, we localized the rat homolog of the von Hippel-Lindau gene (Vhl) to rat chromosome band 4q41.3→q42.1. We also mapped the gene encoding the plasma membrane Ca⁺⁺-transporting ATPase iso-form 2 (Atp2b2) to the same chromosome subregion. These two genes together with Raf1 appear to be members of a large syntenic gene cluster that maps to human chromosome bands 3p25→p26, mouse chromosome bands 6 C3→E, and rat chromosome bands 4q41→q42. Cytogenetic analysis of NRK 52E cells derived from immortalized normal rat kidney epithelial cells revealed an inverted duplication of the region containing this gene cluster.

Polymerase chain reaction–single‐strand conformation polymorphism analysis for the VHL gene in chemically induced kidney tumors of rats using intron‐derived primers

“…The rat VHL gene contains three exons encoding 185 amino acids [11]. It has been mapped to the rat chromosome 4q41.3-42.1 region [12]. The complete rat VHL cDNA sequence (Long-Evans strain) is available from Kikuchi et al [11] and GenBank (accession number U14746).…”

Assignment of the human P532 gene (HERC1) to chromosome 15q22 by fluorescence in situ hybridization

“…Metaphases were prepared following standard cytogenetic procedures. Probe labeling and detection was essentially performed as previously described (Aldaz et al, 1995). In order to confirm chromosome location, a second hybridization was performed utilizing, in addition to the biotin labeled P532 probe, a centromeric specific chromosome 15 alpha satellite probe D15Z (Oncor, Gaithesburg, MD).…”

Cloning and chromosomal localization of the rat <i>Stat5</i> and Y<i>γ</i>1 genes

“…Metaphases were prepared following standard cytogenetic procedures. Probe labeling was performed by nick translation using biotin-11-dlJTP FISH and probe detection was basically performed following Pinkel et al ( 1986) and Aldaz et al ( 1995). (Fig.…”