Colonic aberrant crypt formation accompanies an increase of opportunistic pathogenic bacteria in C57BL/6 mice fed a high-fat diet

Zeng, Huawei; Ishaq, Suzanne L.; Liu, Zhenhua; Bukowski, Michael R.

doi:10.1016/j.jnutbio.2017.11.001

Cited by 50 publications

(50 citation statements)

References 77 publications

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“…This result is consistent with previous literatures. One study showed a decrease of Barnesiella , a SCFA-producing bacteria, in the colon of HFD-fed mice, which may be linked to the gut inflammatory phenotype in azoxymethane-induced colon cancer ( Zeng et al, 2018 ). Likewise, the present study shows the age-specific reduction of potentially beneficial Barnesiella spp.…”

Section: Resultsmentioning

confidence: 99%

Microbial Changes and Host Response in F344 Rat Colon Depending on Sex and Age Following a High-Fat Diet

et al. 2018

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Gut microbiota, an important component that affects host health, change rapidly and directly in response to altered diet composition. Recently, the role of diet–microbiome interaction on the development of colon cancer has been the focus of interest. Colon cancer occurs more frequently in an aged population, and in males. However, the effect of dietary changes on the gut microbiome has been studied mainly in young males, even though it may vary with age and sex. The aim of this study was to investigate microbial changes and host response in the colons of male and female 6-week-old (young) and 2-year-old (old) Fisher-344 rats exposed to a high-fat diet (HFD). Our results showed that exposure to HFD for 8 weeks decreased the species richness of microbiota (Chao1) and increased Firmicutes/Bacteroidetes ratio only in aged rats, and not in young rats. Sex differences underlying the alteration by HFD in the gut microbiome were observed in the microbiome of aged rats. For instance, the abundance ratio of Akkermansia muciniphila and Desulfovibrio spp. increased in response to HFD in young rats and female aged rats, but not in male aged rats. Histological inflammation and cell proliferation of colon mucosa (indexed by Ki67) were significantly increased by HFD even in young rats; aged rats showed significantly higher cell proliferation in the HFD group than in the control. The HFD-induced decrease of species richness and the increase in specific species (Desulfovibrio spp. and Clostridium lavalense), which produce carcinogenic compounds such as H2S and N-nitroso compounds, were significantly correlated with Ki67 index. In colon mucosa, the concentration of myeloperoxidase was increased by HFD only in males, and not in females. In conclusion, the results suggest a link between HFD-induced gut dysbiosis (particularly the low species richness and high abundance ratios of Desulfovibrio spp. and C. lavalense) and cell proliferation of colon mucosa (indicated by Ki67 IHC). In addition, sex differences influence the response of gut microbiome to HFD particularly in old age. Such sex differences in the gut microbiota might be related to sex differences in inflammation in the colon mucosa.

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Section: Resultsmentioning

confidence: 99%

Microbial Changes and Host Response in F344 Rat Colon Depending on Sex and Age Following a High-Fat Diet

et al. 2018

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“…In addition to an elevated level of secondary BAs, consumption of a high-fat diet not only increases inflammatory status but also accompanies an increase of opportunistic pathogenic bacteria in the mouse colon [49,157]. Colonic inflammation and cancer are multifactorial disorders that may be affected by the complex interactions between genetics, diet, the gut microbiome, and other factors [158,159].…”

Section: Secondary Bas Scfas Oncomicrobes and Colon Cancermentioning

confidence: 99%

Secondary Bile Acids and Short Chain Fatty Acids in the Colon: A Focus on Colonic Microbiome, Cell Proliferation, Inflammation, and Cancer

Zeng

Umar

Rust

et al. 2019

IJMS

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340

201

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Secondary bile acids (BAs) and short chain fatty acids (SCFAs), two major types of bacterial metabolites in the colon, cause opposing effects on colonic inflammation at chronically high physiological levels. Primary BAs play critical roles in cholesterol metabolism, lipid digestion, and host–microbe interaction. Although BAs are reabsorbed via enterohepatic circulation, primary BAs serve as substrates for bacterial biotransformation to secondary BAs in the colon. High-fat diets increase secondary BAs, such as deoxycholic acid (DCA) and lithocholic acid (LCA), which are risk factors for colonic inflammation and cancer. In contrast, increased dietary fiber intake is associated with anti-inflammatory and anticancer effects. These effects may be due to the increased production of the SCFAs acetate, propionate, and butyrate during dietary fiber fermentation in the colon. Elucidation of the molecular events by which secondary BAs and SCFAs regulate colonic cell proliferation and inflammation will lead to a better understanding of the anticancer potential of dietary fiber in the context of high-fat diet-related colon cancer. This article reviews the current knowledge concerning the effects of secondary BAs and SCFAs on the proliferation of colon epithelial cells, inflammation, cancer, and the associated microbiome.

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“…The reduction of Anaeroplasmataceae could decrease the pathogenesis of depression. In the study of colon cancer, Zeng et al [59] found that the abundance of Anaeroplasma increased in the HFD-azoxymethane (AOM) group. The Anaeroplasma bacteria is negative by Gram stain, which belongs to Mollicutes class, Tenericutes phylum.…”

Section: Discussionmentioning

confidence: 99%

Rubidium chloride modulated the fecal microbiota community in mice

Chen

Zhuo

et al. 2020

Preprint

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Background: The microbiota plays an important role in host health. Although rubidium (Rb) has been used to study for depression and cancers, the interaction between microbial commensals and Rb is still unexplored. To gain the knowledge of the relationship between Rb and microbes, 51 mice receiving RbCl-based treatment and 13 untreated mice were evaluated of their characteristics and bacterial microbiome changes.Results: The 16S ribosomal RNA gene sequencing of feces showed RbCl generally maintained fecal microbial community diversity, while the shifts in fecal microbial composition were apparent after RbCl exposure for the first time. RbCl significantly enhanced the abundances of Rikenellaceae, Alistipes, Clostridium XlVa and sulfate-reducing bacteria including Deltaproteobacteria, Desulfovibrionales, Desulfovibrionaceae and Desulfovibrio. While, RbCl significantly inhibited the abundances of Tenericutes, Mollicutes, Anaeroplasmatales, Anaeroplasmataceae and Anaeroplasma lineages. Besides, with regarding to the composition of archaea, RbCl significantly enhanced the abundances of Crenarchaeota, Thermoprotei, Sulfolobales, Sulfolobaceae and Sulfolobus lineages. Conclusions: These results revealed that enrichments of Clostridium XlVa and Alistipes could affect the levels of serotonin, a critical signaling molecule of brain-gut-microbiota axis. Therefore, anticancer and anti-depressant effects of RbCl might be partly mediated by modifying brain-gut-microbiota axis.

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Colonic aberrant crypt formation accompanies an increase of opportunistic pathogenic bacteria in C57BL/6 mice fed a high-fat diet

Cited by 50 publications

References 77 publications

Microbial Changes and Host Response in F344 Rat Colon Depending on Sex and Age Following a High-Fat Diet

Microbial Changes and Host Response in F344 Rat Colon Depending on Sex and Age Following a High-Fat Diet

Secondary Bile Acids and Short Chain Fatty Acids in the Colon: A Focus on Colonic Microbiome, Cell Proliferation, Inflammation, and Cancer

Rubidium chloride modulated the fecal microbiota community in mice

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