Colonic Bacteria-Transformed Catechin Metabolite Response to Cytokine Production by Human Peripheral Blood Mononuclear Cells

Krishnamoorthy, Rajapandiyan; Adisa, Abdulraheem R.; Periasamy, Vaiyapuri Subbarayan; Athinarayanan, Jegan; Subash-Babu, Pandurangan; Alshatwi, Ali A.

doi:10.3390/biom9120830

Cited by 3 publications

(4 citation statements)

References 53 publications

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“…Both catechins and their microbial metabolites are known to be able to act as immunomodulators. 51 , 52 Modification of the colonic immune status will further influence the gut microbiota composition.…”

Section: Resultsmentioning

confidence: 99%

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Reciprocal Interactions between Epigallocatechin-3-gallate (EGCG) and Human Gut Microbiota In Vitro

Liu

Bruijn

Bruins

et al. 2020

J. Agric. Food Chem.

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Interaction of tea phenolics with gut microbiota may play an integral role in the health benefits of these bioactive compounds, yet this interaction is not fully understood. Here, the metabolic fate of epigallocatechin-3-gallate (EGCG) and its impact on gut microbiota were integrally investigated via in vitro fermentation. As revealed by ultrahigh performance liquid chromatography hybrid quadrupole Orbitrap mass spectrometry (UHPLC-Q-Orbitrap-MS), EGCG was promptly degraded into a series of metabolites, including 4-phenylbutyric acid, 3-(3′,4′-dihydroxyphenyl)propionic acid, and 3-(4′-hydroxyphenyl)propionic acid, through consecutive ester hydrolysis, C-ring opening, A-ring fission, dehydroxylation, and aliphatic chain shortening. Microbiome profiling indicated that, compared to the blank, EGCG treatment resulted in stimulation of the beneficial bacteria Bacteroides , Christensenellaceae , and Bifidobacterium . Additionally, the pathogenic bacteria Fusobacterium varium , Bilophila , and Enterobacteriaceae were inhibited. Furthermore, changes in concentrations of metabolites, including 4-phenylbutyric acid and phenylacetic acid, were strongly correlated with changes in the abundance of specific gut microbiota. These reciprocal interactions between EGCG and gut microbiota may collectively contribute to the health benefits of EGCG.

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Section: Resultsmentioning

confidence: 99%

“…Third, the role of human intestinal immunity has not been taken into account in this study. Both catechins and their microbial metabolites are known to be able to act as immunomodulators. , Modification of the colonic immune status will further influence the gut microbiota composition.…”

Section: Resultsmentioning

confidence: 99%

Reciprocal Interactions between Epigallocatechin-3-gallate (EGCG) and Human Gut Microbiota In Vitro

Liu

Bruijn

Bruins

et al. 2020

J. Agric. Food Chem.

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show abstract

“…[ 35,60] Despite of the fact that static models are useful tools to study gut microbiota-food interactions, they have limitations when it comes to mimicking gut fermentation as a dynamic process [7,11]; for that reason, great effort has been made to develop dynamic colonic methods.…”

Section: Main Objective Study Subject Referencesmentioning

confidence: 99%

In Vitro Simulation of Human Colonic Fermentation: A Practical Approach towards Models’ Design and Analytical Tools

et al. 2021

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The human colonic microbiota plays an important role in the food digestion process and has a key role in maintaining health status. This community of microbes is inter-individually different due to several factors that modulate its composition. Among them, diet is one of the most relevant, which, in turn, is affected by environmental, economic, and cultural considerations. These pieces of evidence have promoted the study of the influence of diet on gut microbiota and the development of in vitro models that simulate the colonic digestion of foods. This narrative review aims to present a technical approach of the in vitro gut models available to evaluate the impact of diet on human colonic microbiota. A description and comments on the main characteristics, parameters, applicability, faecal inoculum preparation, and analytical tools are made. Despite the progress of in vitro colonic digestion models and metaomic applicability in this research field, there are still some challenges to face due to the lack of a consensus on the methodologies to conduct in vitro colonic digestions and the need to integrate the metaomic data to fully understand the influence of food in human colonic microbiota.

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“…Tea ( Camellia sinensis ) is a widely consumed beverage around the world due to its multiple health benefits [ 22 ]. Several lines of studies have proven that green tea imparts numerous biological functions, such as antioxidant and anti-inflammatory activities [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

Effects of Different Green Tea Extracts on Chronic Alcohol Induced‐Fatty Liver Disease by Ameliorating Oxidative Stress and Inflammation in Mice

Zhou

et al. 2021

Oxidative Medicine and Cellular Longevity

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Alcoholic fatty liver disease (AFLD) is a common chronic liver disease and has become a critical global public health problem. Green tea is a popular drink worldwide and contains several bioactive compounds. Different green teas could contain diverse compounds and possess distinct bioactivities. In the present study, the effects of 10 green teas on chronic alcohol induced-fatty liver disease in mice were explored and compared. The results showed that several green teas significantly reduced triacylglycerol levels in serum and liver as well as the aminotransferase activities in mice at a dose of 200 mg/kg, suggesting that they possess hepatoprotective effects. Moreover, several green teas remarkably decreased the expression of cytochrome P450 2E1, the levels of malondialdehyde and 4-hydroxynonenoic acid, and the contents of proinflammatory cytokines, indicating that they could alleviate oxidation damage and inflammation induced by chronic alcohol exposure. In addition, Seven Star Matcha Tea and Selenium-Enriched Matcha Tea could increase glutathione level. Furthermore, the main phytochemical components in green teas were determined and quantified by high-performance liquid chromatography, and the correlation analysis showed that gallic acid, gallocatechin, catechin, chlorogenic acid, and epigallocatechin gallate might at least partially contribute to protective effects on AFLD. In conclusion, Selenium-Enriched Chaoqing Green Tea, Xihu Longjing Tea, Taiping Houkui Tea, and Selenium-Enriched Matcha Tea showed the strongest preventive effects on AFLD. This research also provides the public with new insights about the effects of different green teas on AFLD.

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Colonic Bacteria-Transformed Catechin Metabolite Response to Cytokine Production by Human Peripheral Blood Mononuclear Cells

Cited by 3 publications

References 53 publications

Reciprocal Interactions between Epigallocatechin-3-gallate (EGCG) and Human Gut Microbiota In Vitro

Reciprocal Interactions between Epigallocatechin-3-gallate (EGCG) and Human Gut Microbiota In Vitro

In Vitro Simulation of Human Colonic Fermentation: A Practical Approach towards Models’ Design and Analytical Tools

Effects of Different Green Tea Extracts on Chronic Alcohol Induced‐Fatty Liver Disease by Ameliorating Oxidative Stress and Inflammation in Mice

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