Colonic lymphoid follicles and <i>NOD2</i>/<i>CARD15</i> mutational status in Crohn's disease

Nascimbeni, Riccardo; Villanacci, Vincenzo; Bassotti, Gabrio; Fisogni, Simona; Gervasi, Maria Clelia; Rossi, Elisa; Lorenzo, Diego Di

doi:10.1002/bjs.6615

Cited by 2 publications

(3 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[16][17][18][19]. Prior studies observed that the number of lymphoid follicles are decreased in disease unaffected colon in CD patients [20,21]. The decreased expression of gene-probes in Cluster 1 is consistent with this observation and suggests that impaired development of lymphoid follicles represents an early pathogenic change in CD.…”

Section: Patient Characteristicssupporting

confidence: 78%

See 1 more Smart Citation

Cluster analysis of genome-wide expression differences in disease-unaffected ileal mucosa in inflammatory bowel diseases

Zhang¹,

DeSimone

Chen³

et al. 2011

2011 IEEE 1st International Conference on Computational Advances in Bio and Medical Sciences (ICCABS)

View full text Add to dashboard Cite

Whole human genome (Agilent) expression profiling was conducted on disease-unaffected ileal RNA collected from the proximal margin of resected ileum from 47 ileal Crohn's disease (CD), 27 ulcerative colitis (UC) and 25 control patients without inflammatory bowel diseases (IBD). Cluster analysis combined with significance analysis of microarrays (SAM) and principal component analysis (PCA)and was used to reduce the data dimension to identify geneprobe clusters associated with early pathogenic changes in ileal CD and UC. Ingenuity Pathway Analysis (IPA) was used to identify the biological pathways associated with each cluster. We reduced the dimensions of the 26,765 gene probe set to 43 gene-probe clusters. Most of these clusters could be labeled as related to different biological pathways, such as Paneth cell antimicrobial peptides, the formation of organized lymphoid structures, or nuclear receptor signaling and xenobiotic metabolism. Molecular phylogenetic 16S rRNA sequence analysis was completed on 83 DNA samples from the same samples used to generate the gene expression profiles. We conducted an exploratory study to determine if the first principle component (PC1) of these clusters could be linked to specific phyla/subphyla taxa.

show abstract

Section: Patient Characteristicssupporting

confidence: 78%

“…Five clusters (#5, 9,13,21,43) were enriched for geneprobes that were down-regulated in UC compared to both Control and ileal CD samples. Three clusters (#7, 26,41) were enriched for gene-probes that were up-regulated in UC compared to both Control and ileal CD samples.…”

Section: Patient Characteristicsmentioning

confidence: 99%