Colonic mucosal replacement by syngeneic small intestinal stem cell transplantation

Growth Factors Adsorbed on Polyglycolic Acid Mesh Augment Growth of Bioengineered Intestinal Neomucosa

“…The clusters contain the putative intestinal stem cells and other cells that comprise the stem cell niche. Transplantation of these intestinal organoids has been shown to generate TESI tissues that resembles native intestine in both structure and function in rats and mice [4][5][6][7][8]. Furthermore, TESI has been demonstrated in rats to reverse clinical malabsorption syndromes [7,9].…”

Intestinal stem cell transplantation

“…Tait et al described a rat model in which they created a freely isolated loop of the ascending colon that kept blood supply intact in the abdomen. By applying surgical mucosectomy to this intestinal segment, they used this anatomical site for transplantation of epithelial cells that were isolated from the small intestine of postnatal rats [25]. Two weeks after seeding the suspension of donor-derived cells over the surface of the colonic loop, they found that the transplants were able to survive ectopically, regenerating the tissue that exhibited the small intestinal phenotype [25].…”

“…By applying surgical mucosectomy to this intestinal segment, they used this anatomical site for transplantation of epithelial cells that were isolated from the small intestine of postnatal rats [25]. Two weeks after seeding the suspension of donor-derived cells over the surface of the colonic loop, they found that the transplants were able to survive ectopically, regenerating the tissue that exhibited the small intestinal phenotype [25]. Stelzner and his group succeeded in orthotopically grafting the epithelial cells that were isolated from the small intestine [26].…”

50 & 100 years ago

“…It has been shown 5,6 that organoids isolated directly from normal intestine contain stem cells, can engraft onto damaged gut epithelium, and can support long-term epithelial regeneration. However, the current study goes one step further by showing that donor stem cells can be expanded in vitro to grow large numbers of organoids, which can then be transplanted and successfully engrafted into a host with a damaged gut.…”