Colonic Sodium-Potassium Adenosine Triphosphate Subunit Gene Expression: Ontogeny and Regulation by Adrenocortical Steroids*

Fuller, Peter J.; Verity, Karen

doi:10.1210/endo-127-1-32

Cited by 71 publications

(32 citation statements)

References 49 publications

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“…The mRNA levels for a and ,B1 in the small intestine are considerably lower at birth than in adult rats and are increased by glucocorticoids (8). Similar results were obtained for rat colon (10). Four isoforms of the subunit have also been described in various tissues (2,3,5,9,(11)(12)(13)(14)(15).…”

Section: Introductionsupporting

confidence: 82%

Na,K-ATPase in diabetic rat small intestine. Changes at protein and mRNA levels and role of glucagon.

Barada¹,

Okolo²,

Field³

et al. 1994

J. Clin. Invest.

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Section: Introductionsupporting

confidence: 82%

Na,K-ATPase in diabetic rat small intestine. Changes at protein and mRNA levels and role of glucagon.

Barada¹,

Okolo²,

Field³

et al. 1994

J. Clin. Invest.

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“…Data supporting this conclusion have recently been published by Nanthakumar and Henning (32) and Beckett et al (33) for the glucocorticoid induction of sucrase. In contrast to sucrase and alkaline phosphatase, CBX stimulated Na,K-ATPase activity in the colon but not in the small intestine, even though glucocorticoids have been shown to stimulate this enzyme activity in both the small and large intestines (13)(14)(15). Two possibilities may account for this discrepancy.…”

Section: Discussionmentioning

confidence: 93%

“…With regard to cell differentiation, weaning is characterized by phenotypic changes in enterocytes that include developmental changes in the activity of various enzymes influenced by the hormonal milieu, including glucocorticoids (1,5). For example, glucocorticoids have been shown to affect the developmental patterns of sucrase (10,11), alkaline phosphatase (12), and Na,K-ATPase (13)(14)(15).…”

mentioning

confidence: 99%

Carbenoxolone Accelerates Maturation of Rat Intestine

2003

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The rat undergoes profound maturational changes in the intestinal structure and function during the third week of its life. To investigate the role of peripheral glucocorticoid metabolism in this process, we studied the postnatal maturation of intestinal structure and function. The peripheral metabolism of glucocorticoids depends on enzyme 11␤-hydroxysteroid dehydrogenase (11␤HSD), which is responsible for the interconversion of corticosterone to 11-dehydrocorticosterone and thus for the modulation of glucocorticoid access to corticosteroid receptors. The pups were treated with carbenoxolone (CBX), an inhibitor of 11␤HSD, for 10 d during the suckling (days 8 -18) or weaning period (days 14 -24 or days 20 -30), and we determined the parameters of intestinal growth and activities of sucrase, alkaline phosphatase, and Na,K-ATPase. The CBX treatment increased plasma concentrations of corticosterone as a result of a significant reduction of peripheral degradation of corticosterone catalyzed by 11␤HSD. This also stimulated intestinal growth without changing somatic growth. The mucosal cell mass was significantly higher in CBX-treated suckling rats, whereas the effect of this treatment was less obvious in weanling animals. CBX increased the crypt depth and villus height in 18-and 24-d-old pups but not in 30-d-old animals. The small intestinal activities of sucrase, alkaline phosphatase, and Na,K-ATPase were not influenced by CBX. In contrast, colonic Na,K-ATPase was stimulated by CBX. We conclude that the administration of CBX results in acceleration of intestinal growth and structural maturation without any influence on the developmental pattern of brush-border hydrolases The concentration of glucocorticoids in the plasma plays an important physiologic role in the promotion of normal ontogeny of the human embryo in utero and of rodents during weaning. The rise of plasma glucocorticoids is associated with an accelerated maturation of various tissues (1). The local action of glucocorticoids in target tissues can be modified by changes in peripheral glucocorticoid metabolism. This metabolism depends on the enzyme 11␤-hydroxysteroid dehydrogenase (11␤HSD), which has been recognized to play a key role in regulating the access of glucocorticoids to corticosteroid receptors. Two isoforms of 11␤HSD with different properties and tissue distribution have been described. Type 2 11␤HSD is a high-affinity NAD-dependent isozyme that catalyzes the conversion of cortisol and corticosterone to cortisone and 11-dehydrocorticosterone, respectively, and thus decreases the local concentration of active glucocorticoids and prevents their binding to mineralocorticoid receptors. In contrast, type 1 11␤HSD is a NADP(H)-dependent isoform that exhibits bidirectional activity in vitro but seems to favor 11-reductase activity in intact cells. Whereas type 2 has been predominantly found in the kidney, colonic epithelium, and placenta, type 1 is the predominant type in the liver, fibroblasts, and cells of lamina propria (2).It has recently been sho...

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“…Therefore (40) found that an increase in expression of a subunits coincided with an increase in the expression of the 3 subunit, with a 1 and 3 levels most closely coordinated. The analysis of rat colonic Na+,K+-ATPase (43) revealed that a 1 and 3 subunit mRNA expression was low at birth, but reached adult levels by day 25 …”

Section: Discussionmentioning

confidence: 99%

Expression and developmental regulation of Na+,K+ adenosine triphosphatase in the rat small intestine.

Zemelman¹,

Walker

Chu

1992

J. Clin. Invest.

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The Na+,K+-ATPase ion pump plays a critical role in fluid and electrolyte physiology of the small intestine. Here we show that, of the three known a isotypes (al, a2, and a3) of the sodium pump found in the rat, only al is expressed in the small intestine. The expression of this isotype, considered at the level of mRNA, is under developmental control, with the adult intestine exhibiting approximately a threefold increase in al message over the neonate. Cortisone treatment of the neonate results in near-adult levels of al mRNA expression. An increase in the abundance of al isotype parallels the changes in its mRNA expression. ,B subunit mRNA is expressed coordinately with the al subunit mRNA. A four-to five-fold rise in the Na+,K+-ATPase activity is also developmentally induced. (J.

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Colonic Sodium-Potassium Adenosine Triphosphate Subunit Gene Expression: Ontogeny and Regulation by Adrenocortical Steroids*

Cited by 71 publications

References 49 publications

Na,K-ATPase in diabetic rat small intestine. Changes at protein and mRNA levels and role of glucagon.

Na,K-ATPase in diabetic rat small intestine. Changes at protein and mRNA levels and role of glucagon.

Carbenoxolone Accelerates Maturation of Rat Intestine

Expression and developmental regulation of Na+,K+ adenosine triphosphatase in the rat small intestine.

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