Colonisation bactérienne vaginale anormale et implantation en assistance médicale à la procréation

Wittemer, C.; Bettahar-Lebugle, K.; Ohl, J.; Rongières, C; Viville, Stéphane; Nisand, I.

doi:10.1016/j.gyobfe.2003.11.017

Cited by 27 publications

(8 citation statements)

References 16 publications

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“…Moreover, the presence of non- Lactobacillus bacteria in the endometrium has already been associated with adverse reproductive outcomes and implantation failure [27]. Likewise, Wittemer et al [28] reported that the presence of endocervical microorganisms (such as Candida albicans , Ureaplasma urealyticum , G vaginalis ) may interfere with embryonic implantation process, thus affecting IVF outcomes. In our cohort, we found a proportion of vaginal G vaginalis in 2 of the idiopathic infertile women.…”

Section: Discussionmentioning

confidence: 99%

Differential Composition of Vaginal Microbiome, but Not of Seminal Microbiome, Is Associated With Successful Intrauterine Insemination in Couples With Idiopathic Infertility: A Prospective Observational Study

Amato

Papaleo

Pasciuta

et al. 2019

Open Forum Infectious Diseases

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Background Vaginal and seminal microbiomes have gained increasing interest for their involvement in reproductive health and fertility. However, their role in reproductive outcome is not fully understood yet. In this study, we aimed to correlate the vaginal and the seminal microbiome of 23 couples with idiopathic infertility to the clinical pregnancy rate after intrauterine insemination (IUI). Methods Vaginal swabs and seminal fluids were collected on the day of IUI procedure and analyzed through polymerase chain reaction amplification of variable regions 3 and 4 (V3–V4) of 16S ribosomal ribonucleic acid genes and Illumina MiSeq sequencing. The taxonomic data were then correlated to IUI success. Results Idiopathic infertile women showed a different average composition of vaginal microbiome compared with control sequences, whereas for seminal counterpart no relevant differences were observed. Furthermore, among idiopathic infertile women, different patterns of Lactobacillus species dominations were observed, with a predominance either of Lactobacillus crispatus, a marker of a healthy vaginal ecosystem, or of Lactobacillus iners and Lactobacillus gasseri, associated with a more dysbiosis-prone environment. More important, considering all investigated variables, vaginal L crispatus domination was the only factor strongly associated to IUI success (P = .0002). Conclusions Our results strengthen the potential role of L crispatus in promoting a favorable environment for pregnancy and suggest that microbiome characterization could be useful, together with standard clinical and laboratory assessments, in the pre-IUI evaluation of infertile couples.

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Section: Discussionmentioning

confidence: 99%

Differential Composition of Vaginal Microbiome, but Not of Seminal Microbiome, Is Associated With Successful Intrauterine Insemination in Couples With Idiopathic Infertility: A Prospective Observational Study

Amato

Papaleo

Pasciuta

et al. 2019

Open Forum Infectious Diseases

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“…Thus, semen microbiome dysbiosis may lead to diseases. Interestingly, increasing evidence is accumulating, highlighting a role of the semen microbiome in infertility [ 9 , 26 , 27 , 37 , 49 , 57 , 58 ]. However, as for other metagenomic studies, it is important to underline that several analytical and biological variables (i.e., patients’ selection, the geographical location, diet, age, hygiene practices, circumcision status, and sexual activities of the study participants, sampling methods and handling, sequencing strategies, statistical analyses) may affect the reliability of results, leading to interpretation biases and impairing their reproducibility ( Figure 2 ).…”

Section: Discussionmentioning

confidence: 99%

“…To date, few studies have been carried out to assess a correlation between male and female microbiomes [19,49]. Wittemer et al analyzed 951 couples undergoing in vitro fertilization procedures and, by culturing endocervical, vaginal and seminal microbiotas, found not only a lower implantation rate in women with endocervical bacterial growth, but also a reduced pregnancy rate and an increased number of miscarriages in the presence of positive vaginal and/or seminal cultures [49]. Mandar et al analyzed pre-and post-coital vaginal samples and semen samples from the partner, and found that semen communities were significantly different and had a lower bacterial richness compared to vaginal samples [19].…”

Section: The Role Of Semen Microbiome On Reproductionmentioning

confidence: 99%

“… Individual variability factors affecting semen microbiota composition and processes in which semen microbiota has been suggested to play a role [ 12 , 19 , 21 , 23 , 36 , 49 , 55 , 56 , 57 , 58 , 63 ]. The simultaneous evaluation of the female partner’s microbiota should be taken into account considering their mutual influences and the potential effects on couples’ and offspring health [ 64 ].…”

Section: Figurementioning

confidence: 99%

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Microbiota and Human Reproduction: The Case of Male Infertility

et al. 2020

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The increasing interest in metagenomics is enhancing our knowledge regarding the composition and role of the microbiota in human physiology and pathology. Indeed, microbes have been reported to play a role in several diseases, including infertility. In particular, the male seminal microbiota has been suggested as an important factor able to influence couple’s health and pregnancy outcomes, as well as offspring health. Nevertheless, few studies have been carried out to date to deeper investigate semen microbiome origins and functions, and its correlations with the partner’s reproductive tract microbiome. Here, we report the state of the art regarding the male reproductive system microbiome and its alterations in infertility.

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“…Wittemer et al ( 18 ) tried to determine the effects of treatment of vaginal and endocervical infections on IVF outcomes. The authors concluded that suspending the actual IVF cycle seemed more reasonable because of the possible deleterious effects of infection on embryonic implantation process, even if the patient was treated with a proper antimicrobial agent.…”

Section: Discussionmentioning

confidence: 99%

Does screening for vaginal infection have an impact on pregnancy rates in intracytoplasmic sperm injection cycles?

Eldivan

Evliyaoğlu

Ersoy

et al. 2016

tjod

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Objective:Assisted reproduction techniques have become widespread worldwide. Considering their costs, physicians endeavor to improve pregnancy rates. Infections are one of the disrupting problems in this arena. We aimed to investigate the effects of screening for vaginal infection on pregnancy rates in intracytoplasmic sperm injection cycles.Materials and Methods:One hundred twenty patients randomized into two groups for this study. Patients were screened for vaginal infections in group 1, and no screening was performed in group 2. The assisted reproduction outcomes were investigated and compared between the two groups.Results:There was no significant difference between ages, or durations and causes of infertility of patients who conceived and of those who did conceive. Forty-five patients in group 1, and 40 patients in group 2 reached the embryo transfer stage. The rates of conception were 23.5% (n=4) in culture-positive patients (n=17), and 42.9% (n=12) in culture-negative patients (n=28) in group 1. There was no significant difference among patients who were not screened, screen-positive, and screen-negative, in terms of pregnancy rates. None of the patients had Neisseria gonorrhoeae or Trichomonas vaginalis. Bacterial vaginosis was detected in 13 patients, and both bacterial vaginosis and Chlamydia trachomatis were detected in 4 patients. Three of 4 patients who conceived screen-positive and 8 of 12 patients who conceived screen-negative delivered healthily at term.Conclusion:No significant difference was found between patients who were sampled for culture and patients who were not sampled in terms of pregnancy rates. Also, no difference was found between the patients who were culture-negative and patients who were treated with antimicrobials after a culture positive result. Further larger studies are warranted to clarify this issue.

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Colonisation bactérienne vaginale anormale et implantation en assistance médicale à la procréation

Cited by 27 publications

References 16 publications

Differential Composition of Vaginal Microbiome, but Not of Seminal Microbiome, Is Associated With Successful Intrauterine Insemination in Couples With Idiopathic Infertility: A Prospective Observational Study

Differential Composition of Vaginal Microbiome, but Not of Seminal Microbiome, Is Associated With Successful Intrauterine Insemination in Couples With Idiopathic Infertility: A Prospective Observational Study

Microbiota and Human Reproduction: The Case of Male Infertility

Does screening for vaginal infection have an impact on pregnancy rates in intracytoplasmic sperm injection cycles?

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