Colonization and vertical transmission of Streptococcus mutans in Turkish children

Hameş-Kocabaş, E. Esin; Uçar, Füsun; Ersın, Nazan; Uzel, Ataç; Alpöz, Ali Rıza

doi:10.1016/j.micres.2006.03.016

Cited by 25 publications

(37 citation statements)

References 13 publications

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“…Others have reported transmission between family members, such as spouse-spouse, father-child, and sibling-sibling (Saarela et al, 1993;Li and Caufield, 1998;Redmo Emanuelsson and Wang, 1998;Kozai et al, 1999;Emanuelsson and Thornqvist, 2000;van Loveren et al, 2000;Redmo Emanuelsson and Thornqvist, 2001;Nie et al, 2002;Tedjosasongko and Kozai, 2002;Ersin et al, 2004;Hames-Kocabas et al, 2008). Although there have been three reports of transmission between unrelated young infants in nursery schools in China, Brazil, and Japan (Mattos-Graner et al, 2001;Tedjosasongko and Kozai, 2002;Liu et al, 2007), these children ranged in age from 2 mos to 4 yrs, when salivary transfer is more likely than in older children.…”

Section: Discussionmentioning

confidence: 99%

Horizontal Transmission of Mutans Streptococci in Children

et al. 2009

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It has not been established whether transmission of mutans streptococci occurs between unrelated children older than 4 years of age. The aim of the study was to investigate the possible transmission of mutans streptococci genotypes from child to child in kindergarten. We studied 96 children (ages 5-6 yrs) in three San Francisco Bay Area public schools. Mutans streptococci colonies from each child were isolated from selective culture on Mitis Salivarius Sucrose Bacitracin agar. We used arbitrary primed polymerase chain reactions to determine the mutans streptococci genotypes. Two children (not siblings) in each of the three schools (6%) shared an identical amplitype of S. mutans, unique to each pair. The 19 S. sobrinus amplitypes were found in 12 children, and all were unique to each child. The presence of matching genotypes of S. mutans demonstrates horizontal transmission of this species between unrelated children aged 5-6 years.

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Section: Discussionmentioning

confidence: 99%

Horizontal Transmission of Mutans Streptococci in Children

et al. 2009

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“…Studies have shown that mutans streptococci is transmitted between mother and child primarily by vertical transmission and thus is a major risk factor in early and future caries ECC with similar bacterial genotypes being common within a family [20]. Whether the familial associations are due to exposures in a shared environment, parental culture, awareness (health literacy), familial (genetic) factors or whether this liability is related to the disorder itself remains to be determined.…”

Section: Genetic and Familial Predispositionmentioning

confidence: 99%

A Sociobiological Model of Early Childhood Caries: The Role of Allostatic Load

FitzGerald¹,

Gulvartian²,

Ramos‐Gomez³

et al. 2017

Pediatr Dent Care

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Objective: Even though many factors have been linked to increased ECC risk, few studies have specifically presented this information as a single comprehensive model. The purpose of this paper is to review the scope and usefulness of the proposed conceptual model in guiding future research of ECC risk as it relates to allostatic load. Methods:The various social, behavioural, and biological risk factors that emerge from the cumulative effects of chronic stress, defined as allostatic load, are discussed separately before being incorporated into a single sociobiological model of ECC.Results: In addition to biological realities, children experience profound impacts from their environments and family circumstances. The risk of developing ECC is especially pronounced in socially disadvantaged contexts. This information, as it is presented in the proposed conceptual model, reveals unique allostatic factors that work to increase risk at the level of the individual child, family, community, and society. Giving clinicians an idea of these realities has the capacity to more effectively screen and risk stratify children on the basis of dental health in the hopes of ultimately increasing their overall health as well. Conclusion:The proposed model provides a conceptual framework that relates factors of allostatic load to increased ECC risk. It demonstrates that a population-based, interprofessional collaboration strategy is essential to delivering effective prevention and treatment modalities to at-risk children. Future research regarding translational strategies has the capacity to reduce the prevalence of the biological, developmental, and psychosocial risk factors and thus ECC as a whole.

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“…Genotyping was undertaken by arbitrarily primed-PCR (AP-PCR) [26] using primer OPA5 (5′-AGGGGTCTTG-3′) [27]. Each reaction mixture (total volume = 50 μl) contained 5 μl of 10 × Taq buffer, 300 of µM dNTPs, 2 μM of primer, 5 μl of template, and 5 U of Taq DNA polymerase.…”

Section: Genotyping Of Clinical Isolates Of S Mutans By Arbitrarily mentioning

confidence: 99%

Identification of ssDNA aptamers specific to clinical isolates of <italic>Streptococcus mutans</italic> strains with different cariogenicity

Cui¹,

Liu²,

Su³

et al. 2016

ABBS

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Streptococcus mutans, a Gram-positive facultative anaerobic bacterium, is considered to be a major etiological factor for dental caries. In this study, plaques from dental enamel surfaces of caries-active and caries-free individuals were obtained and cultivated for S. mutans isolation. Morphology examination, biochemical characterization, and polymerase chain reaction were performed to identify S. mutans. The cariogenicity of S. mutans strains isolated from clinical specimens was evaluated by testing the acidogenicity, aciduricity, extracellular polysaccharide production, and adhesion ability of the bacteria. Finally, subtractive SELEX (systematic evolution of ligands by exponential enrichment) technology targeting whole intact cells was used to screen for ssDNA aptamers specific to the strains with high cariogenicity. After nine rounds of subtractive SELEX, sufficient pool enrichment was achieved as shown by radioactive isotope analysis. The enriched pool was cloned and sequenced randomly, followed by MEME online and RNA structure software analysis of the sequences. Results from the flow cytometry indicated that aptamers H1, H16, H4, L1, L10, and H19 could discriminate highly cariogenic S. mutans strains from poorly cariogenic strains. Among these, Aptamer H19 had the strongest binding capacity with cariogenic S. mutans strains with a dissociation constant of 69.45 ± 38.53 nM. In conclusion, ssDNA aptamers specific to highly cariogenic clinical S. mutans strains were successfully obtained. These ssDNA aptamers might be used for the early diagnosis and treatment of dental caries.

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Colonization and vertical transmission of Streptococcus mutans in Turkish children

Cited by 25 publications

References 13 publications

Horizontal Transmission of Mutans Streptococci in Children

Horizontal Transmission of Mutans Streptococci in Children

A Sociobiological Model of Early Childhood Caries: The Role of Allostatic Load

Identification of ssDNA aptamers specific to clinical isolates of <italic>Streptococcus mutans</italic> strains with different cariogenicity

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Colonization and vertical transmission of Streptococcus mutans in Turkish children

Cited by 25 publications

References 13 publications

Horizontal Transmission of Mutans Streptococci in Children

Horizontal Transmission of Mutans Streptococci in Children

A Sociobiological Model of Early Childhood Caries: The Role of Allostatic Load

Identification of ssDNA aptamers specific to clinical isolates of &lt;italic&gt;Streptococcus mutans&lt;/italic&gt; strains with different cariogenicity

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Identification of ssDNA aptamers specific to clinical isolates of <italic>Streptococcus mutans</italic> strains with different cariogenicity