Colonization efficiency of different sorghum genotypes by Gluconacetobacter diazotrophicus

Yoon, Vanessa; Tian, Gang; Vessey, J. Kevin; Macfie, Sheila M.; Dangi, O. P.; Kumer, Anand K.; Tian, Lining

doi:10.1007/s11104-015-2653-8

Cited by 18 publications

(8 citation statements)

References 38 publications

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“…Several nitrogen-fixing species, especially of Azospirillum, Herbaspirillum, Gluconacetobacter and Burkholderia genera can colonize the surface or the inner roots of sorghum (Coelho et al, 2009;Luna et al, 2010;Yoon et al, 2016), maize (Roncato-Maccari et al, 2003;Roesch et al, 2006;Montañez et al, 2009) and elephant grass (Videira et al, 2014) among other tropical grasses. But it remains unclear to what extent the plants benefit from N fixed by endophytic diazotrophs or from the production of indole-3-acetic acid (IAA) or other growth-promoting substances that are proven to cause morphological changes in roots (such as increased lateral roots and root hairs); thus, increasing nutrient absorption (Beneduzi et al, 2013;Videira et al, 2014;Alves et al, 2015).…”

Section: Figmentioning

confidence: 99%

Nitrogen fixation of Poaceae and Leguminoseae in a green manure experiment in the Brazilian semiarid region

Neto¹,

Freitas²,

Giongo³

et al. 2017

Aust J Crop Sci

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Mixtures of green manure species, including Leguminosae and Poaceae, planted prior to commercial crops may incorporate more biomass than Leguminosae alone and fix more nitrogen than Poaceae alone. Biomass and nitrogen incorporation of three plant treatmens were compared under irrigation condition. In two mixture treatments, the same seven green manure species were planted together but with different seeding proportions. Treatment (1) (low-legume) had 50% the recommended seeding rate of the legume species (jack beans, cowpea and sunn hemp) and 150% the recommended seeding rate of the non-legume species (maize, pearl millet, sorghum and sun flower). The other treatment (2) (high-legume) had opposite proportions. In the third treatment, no green manure plants were sown and weed plants were allowed to grow. The results showed that biomass (9.1 and 8.2 Mg ha -1 ) and nitrogen accumulation (160 and 161 kg ha -1 ) did not differ between the low-legume and high-legume treatments, respectively, which were 2.5 times higher than those of the weed treatment (3.3 Mg ha -1 and 58.1 kg ha -1 ). Despite the higher non-legume biomass proportion and lower nitrogen concentration, the low-legume treatment symbiotically fixed as much N (52 kg ha -1 ) as the high-legume treatment because sorghum derived more N from the atmosphere (79 %) and maize in the same range as the legume species (37 to 54%). Therefore, sorghum and maize can have high N endophytic fixation and green manure prior to commercial crops can be produced both with legume and Poaceae species mixed together, providing a high input of N and biomass in a short period.

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Section: Figmentioning

confidence: 99%

Nitrogen fixation of Poaceae and Leguminoseae in a green manure experiment in the Brazilian semiarid region

Neto¹,

Freitas²,

Giongo³

et al. 2017

Aust J Crop Sci

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“…Gluconacetobacter diazotrophicus is a nitrogen-fixing endophytic bacterium found colonizing the interior of roots and stems of sugarcane plants ( Saccharum officinarum L; Cavalcante and Döbereiner, 1988 ; Gillis et al, 1989 ; James et al, 1994 ) and also other crops such as sweet potato, pineapple, coffee, elephant grass, and rice ( Jimenez-Salgado et al, 1997 ; Tapia-Hernández et al, 2000 ; Muthukumarasamy et al, 2005 ; Saravanan et al, 2008 ; Rouws et al, 2010 ). Recently, internal tissue colonization of Arabidopsis thaliana and sorghum genotypes by G. diazotrophicus was also reported ( Rangel de Souza et al, 2016 ; Yoon et al, 2016 ). G. diazotrophicus has been used as an endophytic model organism to evaluate plant-bacterial interactions with non-legume host ( Saravanan et al, 2008 ).…”

Section: Introductionmentioning

confidence: 98%

Identification of Genes Involved in Indole-3-Acetic Acid Biosynthesis by Gluconacetobacter diazotrophicus PAL5 Strain Using Transposon Mutagenesis

et al. 2016

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Gluconacetobacter diazotrophicus is a beneficial nitrogen-fixing endophyte found in association with sugarcane plants and other important crops. Beneficial effects of G. diazotrophicus on sugarcane growth and productivity have been attributed to biological nitrogen fixation process and production of phytohormones especially indole-3-acetic acid (IAA); however, information about the biosynthesis and function of IAA in G. diazotrophicus is still scarce. Therefore, the aim of this work was to identify genes and pathways involved in IAA biosynthesis in this bacterium. In our study, the screening of two independent Tn5 mutant libraries of PAL5T strain using the Salkowski colorimetric assay revealed two mutants (Gdiaa34 and Gdiaa01), which exhibited 95% less indolic compounds than the parental strain when grown in LGIP medium supplemented with L-tryptophan. HPLC chromatograms of the wild-type strain revealed the presence of IAA and of the biosynthetic intermediates indole-3-pyruvic acid (IPyA) and indole-3-lactate (ILA). In contrast, the HPLC profiles of both mutants showed no IAA but only a large peak of non-metabolized tryptophan and low levels of IPyA and ILA were detected. Molecular characterization revealed that Gdiaa01 and Gdiaa34 mutants had unique Tn5 insertions at different sites within the GDI2456 open read frame, which is predicted to encode a L-amino acid oxidase (LAAO). GDI2456 (lao gene) forms a cluster with GDI2455 and GDI2454 ORFs, which are predicted to encode a cytochrome C and an RidA protein, respectively. RT-qPCR showed that transcript levels of lao. cccA, and ridA genes were reduced in the Gdiaa01 as compared to PAL5T. In addition, rice plants inoculated with Gdiaa01 showed significantly smaller root development (length, surface area, number of forks and tips) than those plants inoculated with PAL5T. In conclusion, our study demonstrated that G. diazotrophicus PAL5T produces IAA via the IPyA pathway in cultures supplemented with tryptophan and provides evidence for the involvement of an L-amino acid oxidase gene cluster in the biosynthesis of IAA. Furthermore, we showed that the mutant strains with reduction in IAA biosynthesis ability, in consequence of the lower transcription levels of genes of the lao cluster, had remarkable effects on development of rice roots.

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“…Ambawade and Pathade [234] reported Bacillus siamensis as a newly isolated endophytic bacteria from the banana have the ability to produce a significant amount of gibberellic acid with and without tryptophan-supplemented in media. One of the beneficial endophytic bacteria reported for its potential in N 2 fixation along with production of phytohormones especially IAA with important crops such as sugarcane [80], rice [93], A. thaliana [95], and sorghum [235], and various other important crops are G. diazotrophicus. In a study by Rodrigues, Soares [236] identified the genes and pathways involved in biosynthesis of IAA by G. diazotrophicus and finally concluded that G. diazotrophicus PAL5 T synthesizes IAA through the IPyA pathway in cultures supplemented with tryptophan and provides data for the contribution of an L-amino acid oxidase gene cluster in the IAA biosynthesis.…”

Section: Phytohormones Productionmentioning

confidence: 99%

Endophytic nitrogen-fixing bacteria: Untapped treasurer for agricultural sustainability

Rana¹,

Kour²,

Kaur³

et al. 2022

J App Biol Biotech

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Nitrogen (N) is one of the vital elements required for proper growth and development of plants. In the earth's atmosphere, N is available in the form of nitrogen gas (N 2 ) and mostly plants utilize N in the form nitrate (NO 3 -) and ammonium ion (NH 4 + ) which are fixed through the biological process known as N 2 fixation. As N is one of the elements most likely to be limiting to plant growth, this phenomenon provides an alternative to the implementations of chemical fertilizers as source of nutrients which have resulted in the ammonia volatilization, leading to significant impact on global warming in the atmosphere which, further, diverts the focus of scientist to find out eco-friendly technology. Globally, the demand for introducing eco-friendly practices for improving sustainable agriculture productivity has been increased. Since long time, microbes play an important role in providing pollution-free environment. Endophytic microbes being present inside the specific tissues of plants mostly empower in the growth of plants. The endophytic nitrogen-fixing microbe has been well characterized from leguminous as well nonlegume crops. Endophytic bacteria belong to different phyla such as Actinobacteria, Bacteroidetes, Firmicutes, and Proteobacteria. The predominant N 2 -fixing endophytic Burkholderia, Rhizobium, Pseudomonas, Bradyrhizobium, Bacillus, Frankia, Enterobacter, and Azospirillum have been reported from different host plant. Nitrogen-fixing endophytic bacteria has a wide variety of application for maintaining growth of plant, crop yield, and health of soil for sustainable agriculture. The present review focuses on major developments on biodiversity of N-fixing endophytic microbiomes and their role for plant growth promotion and soil health for agroenvironmental sustainability.

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Colonization efficiency of different sorghum genotypes by Gluconacetobacter diazotrophicus

Cited by 18 publications

References 38 publications

Nitrogen fixation of Poaceae and Leguminoseae in a green manure experiment in the Brazilian semiarid region

Nitrogen fixation of Poaceae and Leguminoseae in a green manure experiment in the Brazilian semiarid region

Identification of Genes Involved in Indole-3-Acetic Acid Biosynthesis by Gluconacetobacter diazotrophicus PAL5 Strain Using Transposon Mutagenesis

Endophytic nitrogen-fixing bacteria: Untapped treasurer for agricultural sustainability

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