Colonization efficiency of Pseudomonas putida is influenced by Fis-controlled transcription of nuoA-N operon

Teppo, Annika; Lahesaare, Andrio; Ainelo, Hanna; Samuel, Kadri; Kivisaar, Maia; Teras, Riho

doi:10.1371/journal.pone.0201841

Cited by 5 publications

(2 citation statements)

References 55 publications

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“…However, despite the production of ROS, this complex has been shown to be involved in P. fluorescence WCS365 tomato root tip colonization in gnotobiotic systems via enhancing bacterial competitiveness for nutrients [207]. In P. putida, the nuoA-N knockout in the fis-overexpression background (Fis elevates transcription from the nuoA-N promoter) increases the ROS-tolerance and adherence to barley roots [208].…”

Section: Interactions Of P Putida With Eukaryotic Hostsmentioning

confidence: 99%

Narrative of a versatile and adept species Pseudomonas putida

Kivisaar

2020

Journal of Medical Microbiology

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Pseudomonas putida is a fast-growing bacterium found mostly in temperate soil and water habitats. The metabolic versatility of P. putida makes this organism attractive for biotechnological applications such as biodegradation of environmental pollutants and synthesis of added-value chemicals (biocatalysis). This organism has been extensively studied in respect to various stress responses, mechanisms of genetic plasticity and transcriptional regulation of catabolic genes. P. putida is able to colonize the surface of living organisms, but is generally considered to be of low virulence. A number of P. putida strains are able to promote plant growth. The aim of this review is to give historical overview of the discovery of the species P. putida and isolation and characterization of P. putida strains displaying potential for biotechnological applications. This review also discusses some major findings in P. putida research encompassing regulation of catabolic operons, stress-tolerance mechanisms and mechanisms affecting evolvability of bacteria under conditions of environmental stress.

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Section: Interactions Of P Putida With Eukaryotic Hostsmentioning

confidence: 99%

Narrative of a versatile and adept species Pseudomonas putida

Kivisaar

2020

Journal of Medical Microbiology

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“…(23,28,29). These determinants include reactive oxygen species (ROS) tolerance (30,31), chemotaxis toward root exudates (32)(33)(34), siderophore biosynthesis and uptake (35)(36)(37)(38), outer membrane integrity (39), cyclic lipopeptides biosynthesis (40), nitrate reduction (41,42), phase variation (43,44), transport and metabolism of carbon and nitrogen sources (45), and antibiotic production (46,47). Some of the most-studied antibiotics produced by plant-beneficial Pseudomonas spp.…”

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Metabolic and Genomic Traits of Phytobeneficial Phenazine-ProducingPseudomonasspp. Are Linked to Rhizosphere Colonization inArabidopsis thalianaandSolanum tuberosum

Zboralski

Biessy

Savoie

et al. 2020

Appl Environ Microbiol

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Bacterial rhizosphere colonization is critical for phytobeneficial rhizobacteria such as phenazine-producing Pseudomonas spp. To better understand this colonization process, potential metabolic and genomic determinants required for rhizosphere colonization were identified using a collection of 60 phenazine-producing Pseudomonas strains isolated from multiple plant species and representative of the worldwide diversity. Arabidopsis thaliana and Solanum tuberosum (potato) were used as host plants. Bacterial rhizosphere colonization was measured by quantitative PCR using a newly designed primer pair and TaqMan probe targeting a conserved region of the phenazine biosynthetic operon. The metabolic abilities of the strains were assessed on 758 substrates using Biolog phenotype microarray technology. These data, along with available genomic sequences for all strains, were analyzed in light of rhizosphere colonization. Strains belonging to the P. chlororaphis subgroup colonized the rhizospheres of both plants more efficiently than strains belonging to the P. fluorescens subgroup. Metabolic results indicated that the ability to use amines and amino acids was associated with an increase in rhizosphere colonization capability in A. thaliana and/or in S. tuberosum. The presence of multiple genetic determinants in the genomes of the different strains involved in catabolic pathways and plant-microbe and microbe-microbe interactions correlated with increased or decreased rhizosphere colonization capabilities in both plants. These results suggest that the metabolic and genomic traits found in different phenazine-producing Pseudomonas strains reflect their rhizosphere competence in A. thaliana and S. tuberosum. Interestingly, most of these traits are associated with similar rhizosphere colonizing capabilities in both plant species. IMPORTANCE Rhizosphere colonization is crucial for plant growth promotion and biocontrol by antibiotic-producing Pseudomonas spp. This colonization process relies on different bacterial determinants which partly remain to be uncovered. In this study, we combined a metabolic and a genomic approach to decipher new rhizosphere colonization determinants which could improve our understanding of this process in Pseudomonas spp. Using 60 distinct strains of phenazine-producing Pseudomonas spp., we show that rhizosphere colonization abilities correlated with both metabolic and genomic traits when these bacteria were inoculated on two distant plants, Arabidopsis thaliana and Solanum tuberosum. Key metabolic and genomic determinants presumably required for efficient colonization of both plant species were identified. Upon further validation, these targets could lead to the development of simple screening tests to rapidly identify efficient rhizosphere colonizers.

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