Colonization of Arabidopsis thaliana by Herbaspirillum seropedicae promotes its growth and changes its proteomic profile

Leandro, Mariana Ramos; Rangel, Patrícia Louzada; Santos, Tamires Cruz dos; Andrade, Leandro Fernandes; Vespoli, Luciano de Souza; Rangel, Ana Lidia Soares; Souza, Suzane Ariádina de; Barbosa, Roberta Ribeiro; Passamani, Lucas Zanchetta; Silveira, Vanildo; Filho, Gonçalo A. de Souza

doi:10.1007/s11104-019-04236-1

Cited by 5 publications

(6 citation statements)

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“…Although the bacteria colonize only the roots, our findings show that a robust and organ-specific gene expression response is also observed in shoots. Several studies addressing the proteomic and transcriptomic analysis of A. thaliana inoculated by root-specific PGPB also observed the regulation of molecular pathways in shoots, indicating the activation of systemic responses [16,[32][33][34].…”

Section: Discussionmentioning

confidence: 99%

“…During the response to infection by pathogens, plants activate specific PR protein groups with different molecular functions, such as PR-2 (a β-1,3-glucanase), PR-3 (a chitinase family), PR-5 (a thaumatinlike), PR-10 (a ribonuclease-like), PR-12 (a defensin), PR-13 (a thionin), and PR-14 (a lipid-transfer protein) for example [57]. Similarly, multiple studies on PGPB have identified the induction of the PR genes in the host plant [16,33,34]. In our study, PR protein genes were mainly down-regulated in both organs, suggesting the ability of G. diazotrophicus PAL5 to suppress such a plant defense response as a colonization strategy.…”

Section: Discussionmentioning

confidence: 99%

“…Due to its short 6-8 week lifespan, easy maintenance, sequenced genome, small space requirements, and a genome of five chromosomes [14], A. thaliana is a model plant for studies of how plants and microorganisms interact [15]. Several studies have already demonstrated the usefulness of A. thaliana for the characterization of the plant-PGPB association [16][17][18].…”

Section: Introductionmentioning

confidence: 99%

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Fine-Tuning of Arabidopsis thaliana Response to Endophytic Colonization by Gluconacetobacter diazotrophicus PAL5 Revealed by Transcriptomic Analysis

Soares,

Rangel de Souza,

de Souza

et al. 2024

Plants

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Gluconacetobacter diazotrophicus is a diazotrophic endophytic bacterium that promotes the growth and development of several plant species. However, the molecular mechanisms activated during plant response to this bacterium remain unclear. Here, we used the RNA-seq approach to understand better the effect of G. diazotrophicus PAL5 on the transcriptome of shoot and root tissues of Arabidopsis thaliana. G. diazotrophicus colonized A. thaliana roots and promoted growth, increasing leaf area and biomass. The transcriptomic analysis revealed several differentially expressed genes (DEGs) between inoculated and non-inoculated plants in the shoot and root tissues. A higher number of DEGs were up-regulated in roots compared to shoots. Genes up-regulated in both shoot and root tissues were associated with nitrogen metabolism, production of glucosinolates and flavonoids, receptor kinases, and transcription factors. In contrast, the main groups of down-regulated genes were associated with pathogenesis-related proteins and heat-shock proteins in both shoot and root tissues. Genes encoding enzymes involved in cell wall biogenesis and modification were down-regulated in shoots and up-regulated in roots. In contrast, genes associated with ROS detoxification were up-regulated in shoots and down-regulated in roots. These results highlight the fine-tuning of the transcriptional regulation of A. thaliana in response to colonization by G. diazotrophicus PAL5.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Fine-Tuning of Arabidopsis thaliana Response to Endophytic Colonization by Gluconacetobacter diazotrophicus PAL5 Revealed by Transcriptomic Analysis

Soares,

Rangel de Souza,

de Souza

et al. 2024

Plants

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“…After data analysis in ISOQuant, only the proteins that were present or absent (for single proteins) in all three biological replicates were considered in the differential abundance analysis. Data were analyzed using Student's t-test (two-tailed) as previously described by Leandro et al (2019). Specifically, proteins with p-values < 0.05 were considered to be up-accumulated if the fold change (FC) was higher than 1.5 and down-accumulated if the FC was less than 0.667.…”

Section: Proteomic Data Analysismentioning

confidence: 99%

Arabidopsis thaliana exudates induce growth and proteomic changes in Gluconacetobacter diazotrophicus

Santos

Leandro

Maia

et al. 2020

PeerJ

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Background Plants interact with a variety of microorganisms during their life cycle, among which beneficial bacteria deserve special attention. Gluconacetobacter diazotrophicus is a beneficial bacterium able to fix nitrogen and promote plant growth. Despite its biotechnological potential, the mechanisms regulating the interaction between G. diazotrophicus and host plants remain unclear. Methods We analyzed the response of G. diazotrophicus to cocultivation with Arabidopsis thaliana seedlings. Bacterial growth in response to cocultivation and plant exudates was analyzed. Through comparative proteomic analysis, G. diazotrophicus proteins regulated during cocultivation were investigated. Finally, the role of some up-accumulated proteins in the response G. diazotrophicus to cocultivation was analyzed by reverse genetics, using insertion mutants. Results Our results revealed the induction of bacterial growth in response to cocultivation. Comparative proteomic analysis identified 450 bacterial proteins, with 39 up-accumulated, and 12 down-accumulated in response to cocultivation. Among the up-accumulated pathways, the metabolism of pentoses and protein synthesis were highlighted. Proteins potentially relevant to bacterial growth response such as ABC-F-Etta, ClpX, Zwf, MetE, AcnA, IlvC, and AccC were also increased. Reverse genetics analysis, using insertion mutants, revealed that the lack of ABC-F-Etta and AccC proteins severely affects G. diazotrophicus response to cocultivation. Our data demonstrated that specific mechanisms are activated in the bacterial response to plant exudates, indicating the essential role of “ribosomal activity” and “fatty acid biosynthesis” in such a process. This is the first study to demonstrate the participation of EttA and AccC proteins in plant-bacteria interactions, and open new perspectives for understanding the initial steps of such associations.

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“…Herbaspirillum seropedicae is a well-known PGPB that colonizes and benefits non-legume plants such as rice, sorghum, sugarcane, and maize 14 – 18 . Past studies have shown that different strains (e.g., B501, Z67) of H. seropedicae can promote plant growth via nitrogen fixation 15 , 19 – 21 .…”

Section: Introductionmentioning

confidence: 99%

Common gene expression patterns are observed in rice roots during associations with plant growth-promoting bacteria, Herbaspirillum seropedicae and Azospirillum brasilense

Wiggins

Thomas

Rahmatallah

et al. 2022

Sci Rep

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Non-legume plants such as rice and maize can form beneficial associations with plant growth-promoting bacteria (PGPB) such as Herbaspirillum seropedicae and Azospirillum brasilense. Several studies have shown that these PGPB promote plant growth via multiple mechanisms. Our current understanding of the molecular aspects and signaling between plants like rice and PGPB like Herbaspirillum seropedicae is limited. In this study, we used an experimental system where H. seropedicae could colonize the plant roots and promote growth in wild-type rice. Using this experimental setup, we identified 1688 differentially expressed genes (DEGs) in rice roots, 1 day post-inoculation (dpi) with H. seropedicae. Several of these DEGs encode proteins involved in the flavonoid biosynthetic pathway, defense, hormone signaling pathways, and nitrate and sugar transport. We validated the expression pattern of some genes via RT-PCR. Next, we compared the DEGs identified in this study to those we previously identified in rice roots during associations with another PGPB, Azospirillum brasilense. We identified 628 genes that were differentially expressed during both associations. The expression pattern of these genes suggests that some of these are likely to play a significant role(s) during associations with both H. seropedicae and A. brasilense and are excellent targets for future studies.

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Colonization of Arabidopsis thaliana by Herbaspirillum seropedicae promotes its growth and changes its proteomic profile

Cited by 5 publications

References 64 publications

Fine-Tuning of Arabidopsis thaliana Response to Endophytic Colonization by Gluconacetobacter diazotrophicus PAL5 Revealed by Transcriptomic Analysis

Fine-Tuning of Arabidopsis thaliana Response to Endophytic Colonization by Gluconacetobacter diazotrophicus PAL5 Revealed by Transcriptomic Analysis

Arabidopsis thaliana exudates induce growth and proteomic changes in Gluconacetobacter diazotrophicus

Common gene expression patterns are observed in rice roots during associations with plant growth-promoting bacteria, Herbaspirillum seropedicae and Azospirillum brasilense

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