Colonization of human opportunistic Fusarium oxysporum (HOFo) isolates in tomato and cucumber tissues assessed by a specific molecular marker

Abstract

Fusarium oxysporum is a large complex cosmopolitan species composed of plant pathogens, human opportunistic pathogens, and nonpathogenic isolates. Many plant pathogenic strains are known based on host plant specificity and the large number of plant species attacked. F. oxysporum is an opportunistic pathogen in humans with a compromised immune system. The objectives of this study were: (1) to develop a specific marker to detect human opportunistic F. oxysporum (HOFo) isolates; (2) to determine whether or not HOFo isolates can colonize and cause disease symptoms in plants; and (3) to assess Taiwan isolates sensitivity to two agro-fungicides. The primer pair, Primer 5/ST33-R, specifically amplifying Taiwan and international reference HOFo isolates was developed and used to detect and assess the distribution of a Taiwan isolate in inoculated tomato plants and tomato and cucumber fruit. Taiwan HOFo isolate MCC2074 was shown to colonize tomato roots, hypocotyls, and cotyledons, but did not show any visible symptoms. Four days after surface inoculation of tomato and cucumber fruit with the same isolate, MCC2074 was detected in the pericarp and locular cavities of both tomato and cucumber fruit and in columella of tomato fruit. Three Taiwan HOFo isolates were found to be moderately sensitive to azoxystrobin and highly sensitive to difenconazole.

Hexosomal Dispersion: A Nano-Based Approach to Boost the Antifungal Potential of Citrus Essential Oils against Plant Fungal Pathogens

“…Herein, the fungicidal activity of the essential oil obtained from the peels of Citrus lemon , Citrus aurantifolia , Citrus maxima , and Citrus sinensis were assessed against seven pathogenic fungi that have been reported as pathogenic to humans too [ 16 , 17 , 18 , 19 ], namely Rhizoctonia solani , Sclerotium rolfsii , Fusarium solani , Fusarium oxysporum , Fusarium semtectium , Botrytis cinerea , and Alternaria alternata . In the presented study, a nano-based valorization approach is reported to establish a novel hexosomal system containing the most active essential oil, allowing for an enhancement of the efficiency of natural fungicides, reducing costs and increasing competitiveness.…”

Fusarium spp. associated with Chenopodium quinoa crops in Colombia

“…In addition, alarms have been raised in the health sector because FOSC is the second most common and virulent fungus reported, generating pathologies such as keratitis, onychomycosis, dermatitis, and allergies 37 , 38 . Wang et al (2020) reported that FOSC isolated human pathogens could colonize plants without causing disease; therefore, these may be silent reservoirs, increasing the risk of immunosuppressed patients coming into contact with or consuming contaminated fruits and plants; the authors suggest the possibility of gene exchange between a pathogenic strain with a non-pathogenic one, giving virulence to ubiquitous plant fungi 39 . According to Zuriegat et al .…”

Chemical Constituents of a Marine‐derived Fungus Fusarium oxysporum F0888 and their Antibacterial Activity

“…[1,2] Fusarium is a genus of filamentous fungi, which was found in a variety of habitats, such as water sources, soil, or with plants. [3,4] Fusarium oxysporum is the most common and economically important species which include both pathogenic [5] (plant, human, and animal) and non-pathogenic strains. As we known, some strains of F. oxysporum were reported as potential biological control agents, which had biological control activity against fungi, pests and certain insects.…”