Colonization of Lactobacillus rhamnosus GG in Cirrhinus molitorella (Mud Carp) Fingerling: Evidence for Improving Disease Resistance and Growth Performance

Abstract

The use of probiotic bacteria can not only enhance the nutritional utilization of fish feeds to produce more biomass but can also provide a practically “safer” alternative to the fish farming industry to reduce the abuse of antibiotics and drugs. This study investigated the possibility of colonizing Lactobacillus rhamnosus strain GG (LGG) to the intestine of Cirrhinus molitorella (mud carp) fingerling. Colonization of LGG was observed in gut tissue after 14 days of administration with a diet supplemented with 1 × 108 CFU/mL LGG. Moreover, growth performance parameters of the LGG-supplemented diet group, including relative weight gain, feed conversion ratio and feed efficiency, were found about two-fold higher than the control group after 60 days. In addition, fish fed with an LGG-supplemented diet for 60 days showed substantial resistance against the infection of pathogenic bacterial Aeromonas hydrophila, with a relative survival rate of up to 57% compared to the control group. In summary, the results indicated that LGG as dietary supplement for mud carp fingerling can enhance nutrition utilization and better protect fish against the infection of Aeromonas hydrophila. The results provide an insight to the fish farming industry, encouraging a reduction in the use of antibiotics and drugs and the production of “safer” mud carp for the market at a manageable cost.

Enterobacter asburiae E7, a Novel Potential Probiotic, Enhances Resistance to Aeromonas veronii Infection via Stimulating the Immune Response in Common Carp ( Cyprinus carpio )

“…The present results showed that feeding E7-supplemented feed for 28 days significantly diminished the mortality of common carp challenged by A. veronii , reflecting an excellent protective effect in vivo . These data were similar to those of studies showing that diet supplementation with probiotics improved disease resistance in aquatic animals ( 83 , 84 ). However, further studies on the optimal method of administration and probiotic dose are needed to avert probable adverse effects.…”

Probiotic bacteria as a healthy alternative for fish and biological control agents in aquaculture

“…Accordingly, probiotic LAB is considered an immunostimulant in aquatic animals. LAB, as a probiotic stimulates the immune system and improves the quality of the water and nutrition, resulting in a higher rate of larval survival and increased aquaculture production (Yu et al, 2022). Bacillus S11 protects shrimp from disease by activating both cellular and humoral immune defenses inside the shrimp's gut and presumably providing competitive exclusion.…”

“…Dead bacterial cells and yeast-glucan have also been shown to stimulate immune responses in shrimp (Penaeus monodon). Yu et al (2022) found that different strains of Lactobacillus rhamnosus (JCM 1136) induce immune responses in rainbow trout (Oncorhynchus mykiss). Fish-feeding supplements such as probiotics and immunostimulants can prevent infectious diseases.…”