Colonization Pattern of &lt;I&gt;Pasteurella pneumotroplca&lt;/I&gt;in Mice with Latent Pasteurellosis

Mikazuki, Katsumi; Hirasawa, Tsutomu; Chiba, Hiroki; Takahashi, Keiko; Shibata, Yoko; Ohhara, Sayoko; Nenui, Hiroko

doi:10.1538/expanim1978.43.3_375

Cited by 15 publications

(15 citation statements)

References 8 publications

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“…The frequency of this occurrence increased to 51% in DTA 1 /Scnn1b-Tg mice. Strikingly, the only bacterial species identified in the DTA 1 /WT emaciated mice was Pasteurella pneumotropica, an opportunistic pathogen of the oropharynx of mice (29), whereas the Scnn1b-Tg line exhibited pneumonias with a broader species distribution typical of the species that normally colonize the Scnn1b-Tg line. These data support a model whereby mucus clearance (normal in WT mice) is sufficient for clearance of most aspirated bacteria, but that specific bacteria (e.g., P. pneumotropica) escape mucus clearance and require a second component of airways defense (i.e., MF function) for clearance.…”

Section: Discussionmentioning

confidence: 98%

Neonatal Pulmonary Macrophage Depletion Coupled to Defective Mucus Clearance Increases Susceptibility to Pneumonia and Alters Pulmonary Immune Responses

Saini

Wilkinson²,

Terrell³

et al. 2016

Am J Respir Cell Mol Biol

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Resident immune cells (e.g., macrophages [MFs]) and airway mucus clearance both contribute to a healthy lung environment. To investigate interactions between pulmonary MF function and defective mucus clearance, a genetic model of lysozyme M (LysM) promoter-mediated MF depletion was generated, characterized, and crossed with the sodium channel b subunit transgenic (Scnn1b-Tg) mouse model of defective mucus clearance. Diphtheria toxin A-mediated depletion of LysM 1 pulmonary MFs in wild-type mice with normal mucus clearance resulted in lethal pneumonia in 24% of neonates. The pneumonias were dominated by Pasteurella pneumotropica and accompanied by emaciation, neutrophilic inflammation, and elevated Th1 cytokines. The incidence of emaciation and pneumonia reached 51% when LysM 1 MF depletion was superimposed on the airway mucus clearance defect of Scnn1b-Tg mice. In LysM 1 MF-depleted Scnn1b-Tg mice, pneumonias were associated with a broader spectrum of bacterial species and a significant reduction in airway mucus plugging. Bacterial burden (CFUs) was comparable between Scnn1b-Tg and nonpneumonic LysM 1 MF-depleted Scnn1b-Tg mice. However, the nonpneumonic LysM 1 MF-depleted Scnn1b-Tg mice exhibited increased airway inflammation, the presence of neutrophilic infiltration, and increased levels of inflammatory cytokines in bronchoalveolar lavage fluid compared with Scnn1b-Tg mice. Collectively, these data identify key MF-mucus clearance interactions with respect to both infectious and inflammatory components of muco-obstructive lung disease.

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Section: Discussionmentioning

confidence: 98%

Neonatal Pulmonary Macrophage Depletion Coupled to Defective Mucus Clearance Increases Susceptibility to Pneumonia and Alters Pulmonary Immune Responses

Saini

Wilkinson²,

Terrell³

et al. 2016

Am J Respir Cell Mol Biol

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“…pneumotropica is present in many rodent colonies, especially mice, rats and hamsters. The organism is detected typically in the upper respiratory tract, oral cavity, conjunctiva, bladder, mammary glands, genital tract and feces [1,7,8,16,[18][19][20][21]24]. Latent infections are common in both conventional and barrier-maintained colonies [18,19], and other animals and humans have also been infected by this organism [2,17].…”

Section: Discussionmentioning

confidence: 99%

“…In the field of laboratory animal science, P. pneumotropica is generally considered to be a bacterial species that needs microbiological monitoring. On the basis of findings reported by Saito et al [21] and Mikazuki et al [16], specimens for this study were collected from the laryngo-pharyngeal region. To increase the detection accuracy, a confirming amplification including digestion of the PCR products with a restriction enzyme was adopted.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of a Forced-air-ventilated Micro-isolation System for Protection of Mice against Pasteurella pneumotropica.

Hasegawa¹,

Kagiyama

Tajima

et al. 2003

Exp. Anim.

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Studies to date have established that the physical environment inside cages can be controlled adequately by setting the intra-cage ventilation at 60 air changes per hour in a forced-air-ventilated micro-isolation system (FVMIS). In this study, the capability of FVMIS to prevent inter-cage transmission of microorganisms was evaluated using Pasteurella pneumotropica as a reference microorganism. One FVMIS rack and a conventional rack were used, and cages with mice positive for P. pneumotropica and those with P. pneumotropica-free mice were housed on both racks. The mice were examined for P. pneumotropica contamination every 4 weeks after initiating the experiment for 12 weeks using a polymerase chain reaction method. Some P. pneumotropica-free mice housed in open air cages in the conventional rack became positive for P. pneumotropica (four of 28 animals after 4 weeks; eight of 28 animals after 12 weeks), but all P. pneumotropica-free mice housed in the FVMIS cages remained negative for the bacterium throughout the experiment. The results demonstrate that FVMIS can prevent inter-cage transmission of P. pneumotropica when proper cage handling practice is under taken.

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“…There have been some reports of experimental intranasal inoculation models of P. pneumotropica; however, these models might not resemble natural infection [4,11,12] Experimental contact infection resembles natural infection more so than experimental inoculation models [13,14]. There have been some studies on the transmission of this species; however, to our knowledge, experimental contact infection of P. pneumotropica has not been reported [15,16].…”

Section: Introductionmentioning

confidence: 96%

“…The natural hosts of these bacteria are rodents, including mice, rats, hamsters, and gerbils [2][3]. This species colonizes the upper respiratory tract, conjunctiva, intestines, and reproductive tissues of animals [4]. This bacterium causes rodent pasteurellosis, including fatal pneumonia, sepsis and conjunctivitis.…”

Section: Introductionmentioning

confidence: 99%

Experimental Contact Infection of NOD/ShiJic-scid Mice with Pasteurella pneumotropica

Sasaki¹

2017

SOJMID

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Background: Pasteurella pneumotropica is a Gram-negative bacterium that infects the laboratory rodents. In immunodeficient animals, P. pneumotropica infection develops mild to severe or lethal diseases. However, little is known about the transmissibility and pathogenesis of P. pneumotropica in immunodeficient mice. In this study, to assess transmissibility and its pathological conditions by P. pneumotropica, experimental contact infection model of NOD/ShiJic-scid mice was examined with clinical and pathological approaches.

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Colonization Pattern of <I>Pasteurella pneumotroplca</I>in Mice with Latent Pasteurellosis

Cited by 15 publications

References 8 publications

Neonatal Pulmonary Macrophage Depletion Coupled to Defective Mucus Clearance Increases Susceptibility to Pneumonia and Alters Pulmonary Immune Responses

Neonatal Pulmonary Macrophage Depletion Coupled to Defective Mucus Clearance Increases Susceptibility to Pneumonia and Alters Pulmonary Immune Responses

Evaluation of a Forced-air-ventilated Micro-isolation System for Protection of Mice against Pasteurella pneumotropica.

Experimental Contact Infection of NOD/ShiJic-scid Mice with Pasteurella pneumotropica

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