Colonization with enterotoxigenic Bacteroides fragilis is associated with early-stage colorectal neoplasia

Purcell, Rachel; Pearson, John; Aitchison, Alan; Dixon, Liane; Frizelle, Frank; Keenan, Jacqueline I.

doi:10.1371/journal.pone.0171602

Cited by 191 publications

(140 citation statements)

References 27 publications

(28 reference statements)

Supporting

Mentioning

133

Contrasting

Unclassified

Order By: Relevance

“…incorporates a number of different subspecies, there is also the possibility that cats with small cell GI LSA harbor increased numbers of enterotoxigenic Bacteroides spp. as compared to those with IBD, as occurs in humans 68, 69…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Relationship of the mucosal microbiota to gastrointestinal inflammation and small cell intestinal lymphoma in cats

Garraway

Johannes

Bryan

et al. 2018

Veterinary Internal Medicne

View full text Add to dashboard Cite

BackgroundThe gastrointestinal (GI) microbiota in healthy cats is altered in IBD. Little research has been performed to identify whether specific bacterial groups are associated with small cell GI lymphoma (LSA).HypothesisMucosal bacteria, including Enterobacteriaceae and Fusobacterium spp., are abundant in intestinal biopsies of cats with small cell GI LSA compared to cats with IBD.AnimalsFourteen cats with IBD and 14 cats with small cell GI LSA.MethodsRetrospective case control study. A search of the medical records was performed to identify cats diagnosed with IBD and with GI LSA. Bacterial groups identified by FISH in GI biopsies were compared between cohorts and correlated to CD11b+ and NF‐κB expression.Results Fusobacterium spp. (median; IQR bacteria/region) were higher in cats with small cell GI LSA in ileal (527; 455.5 – 661.5; P = .046) and colonic (404.5; 328.8 – 455.5; P = .016) adherent mucus, and combined colonic compartments (free mucus, adherent mucus, attaching to epithelium) (8; 0 – 336; P = .017) compared to cats with IBD (ileum: 67; 31.5 – 259; colon: 142.5; 82.3 – 434.5; combined: 3; 0 – 34). Bacteroides spp. were higher in ileal adherent mucus (P = .036) and 3 combined ileal compartments (P = .034) of cats with small cell GI LSA. There were significant correlations between Fusobacterium spp. totals and CD11b+ cell (P = .009; rs .476) and NF‐κB expression (P = .004; rs .523).ConclusionsThe bacterial alterations appreciated might be influential in development of small cell GI LSA, and should drive further studies to elucidate the effects of microbial‐mediated inflammation on GI cancer progression.

show abstract

Section: Discussionmentioning

confidence: 99%

“…as compared to those with IBD, as occurs in humans. 68,69 The additional 5 probes evaluated using FISH included Clostridium spp. (Erec482), Enterobacteriaceae (Ebac1790), Helicobacter spp.…”

Section: Discussionmentioning

confidence: 99%

Relationship of the mucosal microbiota to gastrointestinal inflammation and small cell intestinal lymphoma in cats

Garraway

Johannes

Bryan

et al. 2018

Veterinary Internal Medicne

View full text Add to dashboard Cite

show abstract

“…Previously, Bacteroides has been implicated in colon cancer by both human association studies and mouse models in which Bacteroides colonization increased colon cancer susceptibility. 45 There may be a direct relationship between these changes and reduced inflammatory cytokine levels in saliva that may benefit patients with cancer when they are treated for a longer duration. Therefore, the findings of the current study offer promising preliminary data to support the hypothesis that APG-157 has an anticancer effect through a microbiome-mediated pathway.…”

Section: Discussionmentioning

confidence: 99%

A randomized, phase 1, placebo‐controlled trial of APG‐157 in oral cancer demonstrates systemic absorption and an inhibitory effect on cytokines and tumor‐associated microbes

Basak

Bera

Yoon

et al. 2020

Cancer

View full text Add to dashboard Cite

Background Although curcumin's effect on head and neck cancer has been studied in vitro and in vivo, to the authors' knowledge its efficacy is limited by poor systemic absorption from oral administration. APG‐157 is a botanical drug containing multiple polyphenols, including curcumin, developed under the US Food and Drug Administration's Botanical Drug Development, that delivers the active components to oromucosal tissues near the tumor target. Methods A double‐blind, randomized, placebo‐controlled, phase 1 clinical trial was conducted with APG‐157 in 13 normal subjects and 12 patients with oral cancer. Two doses, 100 mg or 200 mg, were delivered transorally every hour for 3 hours. Blood and saliva were collected before and 1 hour, 2 hours, 3 hours, and 24 hours after treatment. Electrocardiograms and blood tests did not demonstrate any toxicity. Results Treatment with APG‐157 resulted in circulating concentrations of curcumin and analogs peaking at 3 hours with reduced IL‐1β, IL‐6, and IL‐8 concentrations in the salivary supernatant fluid of patients with cancer. Salivary microbial flora analysis showed a reduction in Bacteroidetes species in cancer subjects. RNA and immunofluorescence analyses of tumor tissues of a subject demonstrated increased expression of genes associated with differentiation and T‐cell recruitment to the tumor microenvironment. Conclusions The results of the current study suggested that APG‐157 could serve as a therapeutic drug in combination with immunotherapy. Lay Summary Curcumin has been shown to suppress tumor cells because of its antioxidant and anti‐inflammatory properties. However, its effectiveness has been limited by poor absorption when delivered orally. Subjects with oral cancer were given oral APG‐157, a botanical drug containing multiple polyphenols, including curcumin. Curcumin was found in the blood and in tumor tissues. Inflammatory markers and Bacteroides species were found to be decreased in the saliva, and immune T cells were increased in the tumor tissue. APG‐157 is absorbed well, reduces inflammation, and attracts T cells to the tumor, suggesting its potential use in combination with immunotherapy drugs.

show abstract

“…fragilis [4], F. nucleatum [32] and Prevotella intermedia [33]. Of the 81 species detected solely using MinION sequencing several were closely related Bacillus, Burkholderia, Streptomyces and Psudomonas species.…”

Section: Detection Of Colorectal Cancer-associated Bacterial Speciesmentioning

confidence: 99%

“…Lifestyle and diet are the strongest risk factors for the disease, and changes in the gut microbiome have been linked to colorectal cancer development and pathogenesis in an increasing number of studies [4].…”

Section: Introductionmentioning

confidence: 99%

MinION Sequencing of colorectal cancer tumour microbiomes – a comparison with amplicon-based and RNA-Sequencing

Taylor

Pearson

Miller

et al. 2019

Preprint

Self Cite

View full text Add to dashboard Cite

Recent evidence suggests a role for the gut microbiome in the development and progression of colorectal cancer. In this study we compare MinION sequencing with meta-transcriptomics and amplicon-based sequencing for microbiome analysis of colorectal tumour tissue samples. DNA and RNA were extracted from 11 colorectal tumour samples. RNA-Sequencing, 16S rRNA amplicon sequencing and MinION genomic sequencing was carried out and resulting data used as input for taxonomic classification using Kraken2. Taxonomic concordance between the three platforms at different taxonomic levels was tested on a per sample basis. The average number of reads per sample using RNA-Sequencing was more than 129 times that generated using MinION sequencing. However, the average read length of MinION sequences was more than 13 times that of RNA or 16S rRNA amplicon sequencing. 16S sequencing was less informative beyond the genus level, and both RNA-Sequencing and MinION sequencing could detect more phyla and genera in the same samples, compared to 16S sequencing. Long-read sequences generated using MinION sequencing can compensate for low numbers of reads for bacterial classification. MinION sequencing can discriminate between bacterial strains and plasmids and shows potential as a tool for microbiome sequencing of colorectal cancers in a clinical setting.

show abstract

Colonization with enterotoxigenic Bacteroides fragilis is associated with early-stage colorectal neoplasia

Cited by 191 publications

References 27 publications

Relationship of the mucosal microbiota to gastrointestinal inflammation and small cell intestinal lymphoma in cats

Relationship of the mucosal microbiota to gastrointestinal inflammation and small cell intestinal lymphoma in cats

A randomized, phase 1, placebo‐controlled trial of APG‐157 in oral cancer demonstrates systemic absorption and an inhibitory effect on cytokines and tumor‐associated microbes

MinION Sequencing of colorectal cancer tumour microbiomes – a comparison with amplicon-based and RNA-Sequencing

Contact Info

Product

Resources

About