Colony‐forming cells in organ cultures of embryonal liver

Latsinik, N. V.; Luria, E. A.; Friedenstein, A. J.; Samoylina, Nina L.; Chertkov, I. L.

doi:10.1002/jcp.1040750205

Cited by 6 publications

(5 citation statements)

References 4 publications

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“…A metaphase spread from donor BM3164 Quantum with chromosomal loss (45,XY,–3) is illustrated in Figure 5A–C, and a metaphase cell from donor BM3164 TCPS with chromosomal gain (47,XY,+ 18) is shown in Figure 5D,E. Structural chromosomal abnormalities were rare (2% in TCPS and 3% in Quantum) and were represented by chromosomal break [46,XY,chrb(10)(p11.2) in Quantum] and reciprocal translocations [46,XY,t(3;6)(q29;p21.1) and 46,XY,t(1;13)(p32;q21) in TCPS, 46,XY,t(16,21) (q13;q21), 46,XY,t(1;15)(p33;q26) and 46,XY, t(3;13)(p15;q31),t(6;7)(p23;q32) in Quantum]. Reciprocal translocations are illustrated in Figure 6A–D for donor BM3164 Quantum and Figure 6E–H for donor 5 TCPS.…”

Section: Resultsmentioning

confidence: 99%

“…in 1966–1970 (1–3), the primary motivation for the therapeutic development of mesenchymal stromal cells (MSCs) continues to be their intrinsic nature for self-renewal and their multi-potency. The demand for research and clinical-grade MSCs necessitates not only that a sufficient quantity of cells be made available but also that these cells should manifest genetic stability for tissue homeostasis.…”

Section: Introductionmentioning

confidence: 99%

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Genetic stability of bone marrow-derived human mesenchymal stromal cells in the Quantum System

et al. 2013

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Background aims The Quantum® Cell Expansion System (Quantum; Terumo BCT, Inc, Lakewood, CO, USA) is a novel hollow fiber-based device that automates and closes the cell culture process, reducing labor intensive tasks such as manual cell culture feeding and harvesting. The manual cell selection and expansion processes for the production of clinical-scale quantities of bone marrow-derived human mesenchymal stromal cells (BM-hMSCs) have been successfully translated onto the Quantum platform previously. The formerly static, manual, in vitro process performed primarily on tissue culture polystyrene substrates may raise the question of whether BM-hMSCs cultured on a hollow fiber platform yields comparable cell quality. Methods A rigorous battery of assays was used to determine the genetic stability of BM-hMSCs selected and produced with the Quantum. In this study, genetic stability was determined by assessing spectral karyotype, micronucleus formation and tumorigenicity to resolve chromosomal aberrations in the stem cell population. Cell phenotype, adherent growth kinetics and tri-lineage differentiation were also evaluated. HMSC bone marrow aspirates, obtained from three approved donors, were expanded in parallel using T225 culture flasks and the Quantum. Results BM-hMSCs harvested from the Quantum demonstrated immunophenotype, morphology and tri-lineage differentiation capacity characteristics consistent with the International Society of Cell Therapy standard for hMSCs. Cell populations showed no malignant neoplastic formation in athymic mice 60 days post-transplant, no clonal chromosomal aberrations were observed and no DNA damage was found as measured by micronucleus formation. Conclusions Quantum-produced BM-hMSCs are of comparable quality and demonstrate analogous genetic stability to BM-hMSCs cultured on tissue culture polystyrene substrates.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Genetic stability of bone marrow-derived human mesenchymal stromal cells in the Quantum System

et al. 2013

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“…1 They are plastic-adherent multipotent cells, characterized by their ability to give rise to colony-forming unit-fibroblasts (CFU-Fs) and to differentiate into osteogenic, adipogenic, and chondrogenic lineages. [2][3][4][5] Aside from the BM, MSCs exist in various connective tissues such as fat, 6 muscle, 7 skin, 8 and placenta. 9 They make up 0.001% to 0.01% of human adult BM cells 10 and can be localized in perivascular spaces.…”

Section: Introductionmentioning

confidence: 99%

Fibrinolytic crosstalk with endothelial cells expands murine mesenchymal stromal cells

Dhahri

Sato-Kusubata

Ohki-Koizumi

et al. 2016

Blood

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Key Points• tPA expands mesenchymal stromal cells (MSCs) in the bone marrow by a cytokine (KitL and PDGF-BB) crosstalk with endothelial cells.• Pharmacologic inhibition of receptor tyrosine kinases (c-Kit and PDGFRa) impairs tPA-mediated MSC proliferation.Tissue plasminogen activator (tPA), aside from its vascular fibrinolytic action, exerts various effects within the body, ranging from synaptic plasticity to control of cell fate. Here, we observed that by activating plasminogen and matrix metalloproteinase-9, tPA expands murine bone marrow-derived CD45

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“…propriedades migratórias e podem originar outras células progenitoras intermediárias e células mais diferenciadas, que vão constituir tecidos ou órgãos (Bianco et al, 2008;Sarugaser et al, 2009;Yadlapalli;Yamashita, 2013 McCulloch e James E Till, (Maximow, 1909;Till;McCulloch, 1961;Friedenstein et al, 1970a;1970b;Friedenstein et al, 1968;Latsinik et al, 1970;Friedenstein, 1980;Friedenshtein;Lurija, 1980;Friedenstein et al, 1974;Friedenstein et al, 1987;Luria et al, 1987 (Till;McCulloch, 1961).…”

Section: Células-troncounclassified

“…Posteriormente, Friedeinstein e colaboradores, reconhecendo os postulados de Maximow, foram os primeiros a isolar essas células da medula óssea (Friedenstein et al, 1970a e b;Friedenstein et al, 1968;Latsinik et al, 1970;Friedenstein, 1980;Friedenshtein;Lurija, 1980;Friedenstein et al, 1974;Friedenstein et al, 1987;Luria et al, 1987;Friedenstein, 1988 (Friedenstein et al, 1970a e b;Friedenstein et al, 1968;Latsinik et al, 1970;Friedenstein, 1980;Friedenshtein;Lurija, 1980;Friedenstein et al, 1974;Friedenstein et al, 1987;Luria et al, 1987;Friedenstein, 1988 (Caplan, 1991;Pittenger et al, 1999).…”

Section: Células-troncounclassified

Células-tronco mesenquimais: isolamento e caracterização de populações derivadas de alvéolo dental humano e identificação e caracterização de populações de polpas dentais de camundongos

Luiz¹

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Dedico também aos meus orientadores do mestrado (Profa. Dra. Vera Cavalcanti de Araújo) e doutorado (Profa. Dra. Andrea Mantesso e Professor Paul Sharpe), que nada mais são grandes inspirações, exemplos de dedicação acadêmica e profissionais que almejo ser um dia.A todos vocês sou eternamente grata por fazerem eu continuar em frente! projeto de pesquisa teve como objetivos duas análises distintas. Na primeira, verificar se existem células-tronco mesenquimais derivadas da curetagem do alvéolo dental humano após extrações dentais. Na segunda, analisar o nicho de células-tronco nas polpas dentais de camundongos. Em comum, as duas análises se basearam no uso de marcadores previamente utilizados na literatura para o estudo de células-tronco. Como não existem marcadores únicos e específicos para a identificação dessas células, diferentes combinações desses marcadores entre si e de técnicas laboratoriais foram empregadas. No primeiro estudo, após o isolamento das células, deu-se especial atenção à sua caracterização, que foi realizada através de ensaios que avaliaram propriedades e comportamentos que sabidamente ocorrem em células-tronco. AGRADECIMENTOSJá na segunda parte desse estudo, utilizamos a polpa dental de camundongos para a análise in vivo de nichos. Em camundongos, a localização do nicho responsável pelo crescimento continuo dos incisivos é conhecida. De uma maneira geral, nossos resultados demonstram que: 1) É possível isolar células-tronco/progenitoras a partir de tecidos curetados de alvéolo dental após extrações dentárias. Esse fato é inédito e abre novas perspectivas em termos de oportunidade de coleta e futura aplicação clínica. 2) É possível observar a diversidade populacional nas células que formam o nicho de células-tronco em polpa dental de camundongos, e através de uma organização hierárquica, propusemos um modelo para este nicho. Esse modelo servirá de base para estudos subsequentes que visem avaliar o comportamento das células que formam o nicho, quando isoladas das demais, e abrirá possibilidade para análises em outros tecidos dentais e não dentais.Palavras-chave: Células-tronco dentais. Osso alveolar. Polpa dental. Nicho de células-tronco There is great interest in the study of stem cells due to their ability to produce mature cells of different lineages that participate actively in the process of homeostasis, injury response, regeneration and tissue repair. The dental pulp is the most studied tissue in dentistry, but several studies have shown the presence of these cells in the periodontal tissue region. It is important to note that depending on their origin, these cells exhibit different behaviours, especially in regard to in vivo transplantation. Furthermore, the microenvironment in which these cells are found (niches) have an essential role in their behaviour as they control important aspects such as differentiation and self-renewal. This research project aimed for two distinct analyses. The first was to verify if there are mesenchymal stem cells derived from human dental socket curettage a...

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Colony‐forming cells in organ cultures of embryonal liver

Cited by 6 publications

References 4 publications

Genetic stability of bone marrow-derived human mesenchymal stromal cells in the Quantum System

Genetic stability of bone marrow-derived human mesenchymal stromal cells in the Quantum System

Fibrinolytic crosstalk with endothelial cells expands murine mesenchymal stromal cells

Células-tronco mesenquimais: isolamento e caracterização de populações derivadas de alvéolo dental humano e identificação e caracterização de populações de polpas dentais de camundongos

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