Color and chemical reactions of selected sesquiterpene lactones and ecdysones from asteraceae on TLC plates

Nowak, Gerard; Urbańska, Maria; Nawrot, Joanna; Bernard, Marek K.; Dawid-Pać, Renata

doi:10.1556/jpc.26.2013.3.15

Cited by 8 publications

(12 citation statements)

References 11 publications

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“…The aliquots were filtered through a cotton filter, gathered, and evaporated to dryness under a rotary evaporator. The procedure for the preliminary thin-layer chromatography (TLC) analysis to confirm the presence of ecdysteroids was carried out as described by Nowak et al (2013).…”

Section: Preliminary Thin Layer Chromatography (Tlc) Analysis For Detmentioning

confidence: 99%

Various in vitro systems of Ragged Robin (Lychnis flos-cuculi L.): a new potential source of phytoecdysteroids?

Maliński

Kikowska

Kruszka

et al. 2019

Plant Cell Tiss Organ Cult

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Lychnis flos-cuculi L. is a species containing ecdysteroids, triterpenoid saponins, flavonoids, and phenolic acids, and therefore is a plant of potential medicinal value. In the presented research, diverse in vitro cultures of this taxon were developed to obtain the uniform material capable of producing ecdysteroids, including micropropagated plantlets, shoot cultures, liquid agitated whole plant cultures with fast-growing roots, and callus. A protocol of micropropagation through axillary bud formation was established using plant growth regulators at different concentrations and combinations. All the variants of plant growth regulator supplementation significantly affected a shoot proliferation rate ranging from 8 to 16 plantlets per explant, depending on the medium; DNA content of all the studied plant materials was similar. The thin-layer chromatography analysis of the extracts revealed the presence of ecdysteroids in every plant material apart from callus. The content of 20-hydroxyecdysone and polypodine B was evaluated by high-performance liquid chromatography. Agitated plantlets were in vitro cultures that efficiently formed abundant root biomass with significant concentrations of ecdysteroids. In vitro-derived adventitious roots contained twofold higher content of ecdysteroids than those of intact plants. The organs of flowering in vitro-propagated plants, transferred to experimental plot, contained twice as much ecdysteroids when compared to the organs of plants from the natural site, among which flowers were the richest in ecdysteroids. The results revealed that adventitious roots from L. flos-cuculi agitated cultures can be considered as an alternative biotechnological source of biomass rich in pharmaceutically active ecdysteroids. Key message Diverse types of Lychnis flos-cuculi in vitro cultures have been developed. HPLC analysis demonstrated much higher ecdysteroid content in cultures and regenerated plants when compared to natural site plants. Keywords Micropropagation • Callus • 20-Hydroxyecdysone • Polypodine B • Agitated plant cultures • Genome size Abbreviations 2,4-D 2,4-Dichlorophenoxyacetic acid 20HE 20-Hydroxyecdysone BAP N 6-benzylaminopurine Dic Dicamba (3,6-dichloro-2-methoxybenzoic acid) IAA Indole-3-acetic acid Kin Kinetin (N 6-furfuryladenine) MS Murashige & Skoog medium NAA 1-Naphthaleneacetic acid PGRs Plant growth regulators Pic Picloram (4-amino-3,5,6-trichloro-2-pyridinecarboxylic acid) polB Polypodine B (5β,20-dihydroxyecdysone) TDZ Thidiazuron (1-phenyl-3-(1,2,3-thiadiazol-5-yl)-urea TLC Thin-layer chromatography Communicated by Ali R. Alan.

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Section: Preliminary Thin Layer Chromatography (Tlc) Analysis For Detmentioning

confidence: 99%

Various in vitro systems of Ragged Robin (Lychnis flos-cuculi L.): a new potential source of phytoecdysteroids?

Maliński

Kikowska

Kruszka

et al. 2019

Plant Cell Tiss Organ Cult

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“…Control chromatograms show that C8 and C8,9 parthenolide derivatives have purple colour [12]. Figure 3 is an example of these differences.…”

Section: Resultsmentioning

confidence: 97%

9α-hydroxyparthenolide in Zoegea leptaurea subsp. mesopotamica (Czerep.) Rech. (Asteraceae)

et al. 2015

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S u m m a r yFrom the aerial parts of Zoegea leptaurea subsp. mesopotamica (Czerep.) Rech. (syn . Zoegea mesopotamica Czerep.), 9α-hydroxyparthenolide was isolated. This compound was identified by spectral methods ( 1 H NMR and 13 C NMR). This research confirmed earlier indications about the presence of 4,5-epoxygermacranolides in the Zoegea L. genus. Thus, distinctive chemistry feature of plants in this taxon has chemotaxonomic implications.

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“…Upon spraying with the anisaldehyde reagent the compounds 6 -11 were isolated from the aerial parts of Stizolophus balsamita. The color of the spots of those germacranolides can be ascribed to the presence of several substituents at the carbon C8 and C9 [6]. Each of the four germacranolides: izospiciformin (7), stizolin (8), 8α,9α-dihydroxyparthenolide (9), and stizolicin (11) appeared as mauve spots on the chromatograms, apparently due to the above mentioned substituents: α-OH substituent on C9 and ester/OH group on C8 (Fig.…”

Section: Tlc Of Germacranolides From Stizolophus Balsamitamentioning

confidence: 95%

“…Of significance are the TLC results for the three most important Asteraceae ecdysones: polypodin B (3), ajugasterone C (4) and 20-hydroxyecdysone (5) which were found in Serratula wolffii. Compound 3 was changing its polarity depending on the presence of the solvents used to develop the chromatogram [6]. Moreover, only compounds 3 and 5 changed their color a few hours after being sprayed upon with the developer (Fig.…”

Section: Tlc Of the Compounds From Serratula Wolffii Herbmentioning

confidence: 99%

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Detection of Pharmacological Active Compounds of the Asteraceae Family and their Chemotaxonomical Implications

Urbańska¹,

Nawrot²,

Dawid-Pać³

et al. 2014

JPS

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It can be assumed that sesquiterpene lactones and natural phytosteroids (ecdysones) are the characteristic compounds of the plants from the Asteraceae family. They display certain pharmacological properties and thus are helpful in chemical descriptions of the Asteraceae tribes, subtribes and species. Also some phenolic glycosides found in genus Klasea Cass, and in genus Centaurea L. may be of medical and chemotaxonomical significance. Our studies on the species of the aforementioned taxons and the isolation of several compounds allowed for interesting conclusions of phytochemical and taxonomical nature.

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Color and chemical reactions of selected sesquiterpene lactones and ecdysones from asteraceae on TLC plates

Cited by 8 publications

References 11 publications

Various in vitro systems of Ragged Robin (Lychnis flos-cuculi L.): a new potential source of phytoecdysteroids?

Various in vitro systems of Ragged Robin (Lychnis flos-cuculi L.): a new potential source of phytoecdysteroids?

9α-hydroxyparthenolide in Zoegea leptaurea subsp. mesopotamica (Czerep.) Rech. (Asteraceae)

Detection of Pharmacological Active Compounds of the Asteraceae Family and their Chemotaxonomical Implications

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