Color Atlas of Medical Bacteriology

Maza, Luis M. de la; Pezzlo, Marie; Bittencourt, Cassiana E.; Peterson, Ellena M.

doi:10.1128/9781683671077

Cited by 23 publications

(27 citation statements)

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“…The specimen was directly cultured on media then identification was completed depending on gram stain, morphological and biochemical tests using IMVIC to ensure diagnosis used a VITEK 2 technique was utilized. (Koneman et al, 2017;Cappuccino and Welsh, 2020;De-Lamaza et al, 2020).…”

Section: Methodsmentioning

confidence: 99%

“…In other word, less immunogenic LPS could serve as therapeutics to antagonize an overreacting innate immune system (Steimle et al, 2016). Enterobacter cloacae belonging to order Enterobacteriales, family Enterobacteriaceae gram negative bacteria bacilli shaped (De-Lamaza et al, 2020). Facultative anaerobic it is related with opportunistic infections that is specific in immunocompromised patients (Hong et al, 2018).and nosocomial infections (Jin et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

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Effect of Lipopolysaccharide Extracted from Enterobacter cloacae on some Pathogenic Bacteria

Aswad¹,

Altaii²

2020

RJS

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A 106 different specimen were collected to isolate and diagnose some gram negative and gram-positive bacteria, as it was performed for the period from 15 August to 30 November 2019 from AL-Salam and Al-Khansaa educational hospitals in Mosul. Lipopolysaccharide (LPS) was extracted from Enterobacter cloacae by using the Methanol-Chloroform method. extract was diagnosed, using some biochemical tests such as the Molisch test to detect carbohydrate content and Folin reagent in order to determine proteins concentration, lipid solubility in organic solvent and Bio drop technique as well as to detect nucleic acid concentration. The extraction methods showed its efficiency with low protein and nucleic acids concentration in the extract. Lipopolysaccharide demonstrated its efficiency inhibiting microbial growth on all test isolates at concentrations 1100, 1000 µg\ ml while its lipopolysaccharide showed its inhibition of Proteus mirabilis at all concentrations used. LPS showed possibility used as inhibitory, antimicrobial growth.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect of Lipopolysaccharide Extracted from Enterobacter cloacae on some Pathogenic Bacteria

Aswad¹,

Altaii²

2020

RJS

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“…All manufactured media were used following the manufacturer guidelines. All WAPHL in-house media use followed the Enterics and Special Bacteriology Reference Units laboratory procedure manuals and microbiology reference books (Holt, 1994; Weyant, 1996; MacFaddin, 2000; de la Maza, 2004; Garcia and Isenberg, 2010; Jorgensen, 2015).…”

Section: Methodsmentioning

confidence: 99%

Laboratory Review of Foodborne Disease Investigations in Washington State 2007–2017

Swoveland

Stewart

Eckmann

et al. 2019

Foodborne Pathogens and Disease

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The Washington State Department of Health Public Health Laboratories (WAPHL) has tested 11,501 samples between 2007 and 2017 for a foodborne disease using a combination of identification, serotyping, and subtyping tools. During this period there were 8037 total clinical and environmental samples tested by pulsed-field gel electrophoresis (PFGE), including 512 foodborne disease clusters and 2176 PFGE patterns of Salmonella enterica subsp. enterica . There were 2446 Shiga toxin–producing Escherichia coli samples tested by PFGE, which included 158 foodborne disease clusters and 1174 PFGE patterns. There were 332 samples of Listeria monocytogenes tested by PFGE, including 35 foodborne disease clusters and 104 PFGE patterns. Sources linked to outbreaks included raw chicken, unpasteurized dairy products, various produce types, and undercooked beef among others. As next-generation sequencing (NGS) replaces PFGE, the impact of this transition is expected to be significant given the enhanced cluster detection power NGS brings. The measures presented here will be a reference baseline in future years.

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“…The intake of unwholesome water could have devastating effects on our health, as unsafe drinking water is a key determinant of many microbial diseases with serious complications in immunocompetent and immunocompromised individuals [ 5 , 6 ]. Many pathogens are readily transmitted through drinking water including Cryptosporidium spp [ 7 , 8 ]. The presence of such parasites in drinking water is partly due to 4 reasons: Firstly, these organisms are indigenous pathogens found in most domestic animals; secondly, the degree of environmental contamination with their infective stages; thirdly, their resistance to water purification processes and finally unhygienic handling of drinking water [ 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

Detection of Cryptosporidium spp. in Diarrheic Immunocompetent Patients in Beni-Suef, Egypt: Insight into Epidemiology and Diagnosis

et al. 2018

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Cryptosporidium species is an important cause of gastrointestinal infections globally. This study aimed to shed light on its role in diarrheic immunocompetent patients in Beni-Suef, Egypt and to compare three diagnostic methods. Two hundred diarrheic patients, 37±16.8 year old, were enrolled. Stool samples were examined by light microscopy, using modified Ziehl-Neelsen stain (MZN) for Cryptosporidium spp. oocysts. Coproantigens were detected by sandwich ELISA. DNA molecular diagnosis was done by nested PCR. PCR yielded the highest detection rates (21.0%), compared to ELISA (12.5%) and MZN staining method (9.5%). The higher infection rates were in 20–40 year-old group, followed by 40–60 year-old. Association between epidemiologic factors was statistically not significant; positivity and gender, clinical manifestations, residence, source or water, or contact with animals. Cryptosporidiosis is an important enteric parasitic infection in Beni-Suef and PCR remains the gold standard for diagnosis.

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Color Atlas of Medical Bacteriology

Cited by 23 publications

References 0 publications

Effect of Lipopolysaccharide Extracted from Enterobacter cloacae on some Pathogenic Bacteria

Effect of Lipopolysaccharide Extracted from Enterobacter cloacae on some Pathogenic Bacteria

Laboratory Review of Foodborne Disease Investigations in Washington State 2007–2017

Detection of Cryptosporidium spp. in Diarrheic Immunocompetent Patients in Beni-Suef, Egypt: Insight into Epidemiology and Diagnosis

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