Color-Based Segmentation vs. Stereology: A Simple Comparison Between Two Semi-Automated Methods of Image Analysis for the Quantification of Collagen

Salinas, Paulo; Sanhueza, J; Sandoval, Carlos

doi:10.4067/s0717-95022018000301118

Cited by 4 publications

(1 citation statement)

References 15 publications

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“…Image analysis was conducted using ImageJ v1.49 software (National Institutes of Health, USA), which facilitated the quanti cation of aligned and non-aligned collagen bers. The "color segmentation" plug-in was employed to distinguish and quantify the different types of collagens based on their color properties and alignment within the tissue (Salinas et al, 2018). This comprehensive approach allowed for a nuanced understanding of the structural and compositional characteristics of vertebral collagen in Common carp.…”

Section: Methodsmentioning

confidence: 99%

Anatomy, 3D micro-CT and Semiquantitative Elemental Microanalysis in Common carp vertebrae (Cyprinus carpio; Linnaeus, 1758)

Salinas,

Naciff,

Navarro

et al. 2024

Preprint

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The study describes the anatomy vertebrae of Common Carp (Cyprinus carpio; Linnaeus, 1758) using 3D micro-CT and Semiquantitative Elemental Microanalysis (EDX), which helps to understand the ontogeny and factors that determine the biomechanics of bones in wild fish. The study of Common carp vertebrae revealed a complex and heterogeneous structure, with a variety of components contributing to their function and stability. The vertebral bodies were composed of lamellar trabeculae and internal hollow spaces, while the neural and hemal arches were composed of bony trabeculae and connected by a lace-like structure. The results of EDX analysis demonstrated the presence of oxygen, nitrogen, sodium, carbon, magnesium, and chlorine, among others. The calcium to phosphorus ratio was within the ranges described in teleost, suggesting good bone health. The presence of magnesium, sodium, potassium, chlorine, and nitrogen is due to their essential role in different biological processes. In addition, a difference was observed between the trabeculae present in the vertebrae of the abdominal and caudal segments, suggesting a relationship between the density of each bone and its function. These findings contribute to the understanding of the structure and function of vertebrae in wild Common carp and may have implications for understanding the evolution of the vertebral column in fish in general.

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Section: Methodsmentioning

confidence: 99%

Anatomy, 3D micro-CT and Semiquantitative Elemental Microanalysis in Common carp vertebrae (Cyprinus carpio; Linnaeus, 1758)

Salinas,

Naciff,

Navarro

et al. 2024

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

Anatomy, 3D micro-CT and semiquantitative elemental microanalysis in common carp vertebrae (Cyprinus carpio; Linnaeus, 1758)

Salinas,

Naciff,

Navarro

et al. 2024

Zoomorphology

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Long-term substitution of fish oil with alternative sources in Atlantic salmon (Salmo salar): Performance, health, and consumer appeal

Leyton,

Marín,

Castillo

et al. 2024

Aquaculture

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Color-Based Segmentation vs. Stereology: A Simple Comparison Between Two Semi-Automated Methods of Image Analysis for the Quantification of Collagen

Abstract: Color-based segmentation vs. stereology: a simple comparison between two semiautomated methods of image analysis for the quantification of collagen.

Cited by 4 publications

References 15 publications

Anatomy, 3D micro-CT and Semiquantitative Elemental Microanalysis in Common carp vertebrae (Cyprinus carpio; Linnaeus, 1758)

Anatomy, 3D micro-CT and Semiquantitative Elemental Microanalysis in Common carp vertebrae (Cyprinus carpio; Linnaeus, 1758)

Anatomy, 3D micro-CT and semiquantitative elemental microanalysis in common carp vertebrae (Cyprinus carpio; Linnaeus, 1758)

Long-term substitution of fish oil with alternative sources in Atlantic salmon (Salmo salar): Performance, health, and consumer appeal

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