Color-coded LED microscopy for multi-contrast and quantitative phase-gradient imaging

Lee, Donghak; Ryu, S.; Kim, Uihan; Jung, Daeseong; Joo, Chulmin

doi:10.1364/boe.6.004912

Cited by 53 publications

(29 citation statements)

References 24 publications

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“…Fortunately, a thin label-free phase object often satisfies this assumption. Previous studies [33][34][35][36] have demonstrated phase retrieval techniques by tuning multiwavelength illumination for nondispersive objects. Even a phase imaging method under a white-light source has been proposed.…”

Section: Framework Of Wavelength Multiplexed Fourier Ptychographic MImentioning

confidence: 99%

“…Even a phase imaging method under a white-light source has been proposed. 37 These methods [33][34][35][36][37] assume that the specimen maintains similar transmission properties across a large bandwidth. In our strategy, the differences of wavelength-dependent resolution vary the sizes of the circular regions in the Fourier plane.…”

Section: Framework Of Wavelength Multiplexed Fourier Ptychographic MImentioning

confidence: 99%

See 1 more Smart Citation

Fourier ptychographic microscopy using wavelength multiplexing

Bian

Suo

et al. 2017

J. Biomed. Opt

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Fourier ptychographic microscopy (FPM) is a recently developed technique stitching low-resolution images in Fourier domain to realize wide-field high-resolution imaging. However, the time-consuming process of image acquisition greatly narrows its applications in dynamic imaging. We report a wavelength multiplexing strategy to speed up the acquisition process of FPM several folds. A proof-of-concept system is built to verify its feasibility. Distinguished from many current multiplexing methods in Fourier domain, we explore the potential of high-speed FPM in spectral domain. Compatible with most existing FPM methods, our strategy provides an approach to high-speed gigapixel microscopy. Several experimental results are also presented to validate the strategy.

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Section: Framework Of Wavelength Multiplexed Fourier Ptychographic MImentioning

confidence: 99%

Section: Framework Of Wavelength Multiplexed Fourier Ptychographic MImentioning

confidence: 99%

Fourier ptychographic microscopy using wavelength multiplexing

Bian

Suo

et al. 2017

J. Biomed. Opt

View full text Add to dashboard Cite

show abstract

“…Assuming a linearized model for a weakly scattering sample, the DPC phase retrieval problem becomes a single-step deconvolution process using the phase contrast transfer function (PTF). By implementing DPC with a programmable LED array [19,20,30] or a programmable LCD panel [31], we are able to realize dynamic QPI along arbitrary axes of asymmetry, without any mechanical moving parts.…”

Section: Introductionmentioning

confidence: 99%

“…However, artifacts of phase reconstruction still cannot be completely avoided, since DPC's PTF is not circularly symmetric with only 2-axis measurements. Efforts have been made toward developing high speed, or even single-shot QPI mechanisms, such as color-coded DPC based on wavelength multiplexing [30,32,33]. Other efforts have been made to achieve isotropic DPC by either using more time-consuming intensity measurements along different illumination angles (12-axis) [20] or replacing the conventional half-circle illumination aperture with 3-axis radially asymmetric patterns [34] or 2-axis gradient amplitude patterns [35].…”

Section: Introductionmentioning

confidence: 99%

Optimal illumination scheme for isotropic quantitative differential phase contrast microscopy

et al. 2019

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Differential phase contrast microscopy (DPC) provides high-resolution quantitative phase distribution of thin transparent samples under multi-axis asymmetric illuminations. Typically, illumination in DPC microscopic systems is designed with 2-axis half-circle amplitude patterns, which, however, result in a non-isotropic phase contrast transfer function (PTF). Efforts have been made to achieve isotropic DPC by replacing the conventional half-circle illumination aperture with radially asymmetric patterns with 3-axis illumination or gradient amplitude patterns with 2-axis illumination. Nevertheless, these illumination apertures were empirically designed based on empirical criteria related to the shape of the PTF, leaving the underlying theoretical mechanisms unexplored. Furthermore, the frequency responses of the PTFs under these engineered illuminations have not been fully optimized, leading to suboptimal phase contrast and signal-to-noise ratio (SNR) for phase reconstruction. In this Letter, we provide a rigorous theoretical analysis about the necessary and sufficient conditions for DPC to achieve perfectly isotropic PTF. In addition, we derive the optimal illumination scheme to maximize the frequency response for both low and high frequencies (from 0 to 2N A ob j ), and meanwhile achieve perfectly isotropic PTF with only 2-axis intensity measurements. We present the derivation, implementation, simulation and experimental results demonstrating the superiority of our method over state-of-the-arts in both phase reconstruction accuracy and noise-robustness.

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“…In time-coded LED array microscopy, multi-contrast images such as bright-field, dark-field, and differential phase contrast (DPC) are acquired by temporally changing the illumination patterns of the light source [18]. In color-coded LED array microscopy, three color-coded illumination allows simultaneous acquisition of bright-field, dark-field, and DPC images at a single shot [19].…”

Section: Introductionmentioning

confidence: 99%

Acquisition of Multi-Modal Images of Structural Modifications in Glass with Programmable LED-Array-Based Illumination

2019

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Ultrashort laser pulses can induce structural modifications in bulk glass, leading to refractive index change and scattering damage. As bright-field, dark-field, and phase imaging each provide complementary information about laser-induced structures, it is often desired to use multiple observations simultaneously. As described herein, we present the acquisition of bright-field, dark-field, and differential phase-contrast images of structural modifications induced in glass by femtosecond laser pulses with an LED array microscope. The contrast of refractive index change can be enhanced by differential phase-contrast images. We also report on the simultaneous acquisition of bright-field and dark-field images of structural modifications in a glass with LED-array-based Rheinberg illumination. A single-shot color image is separated to obtain bright field and dark field images simultaneously. We provide an experimental demonstration on multi-modal imaging of structural modifications in a glass with an LED array microscope using temporally-coded illumination and color-coded illumination.

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Color-coded LED microscopy for multi-contrast and quantitative phase-gradient imaging

Cited by 53 publications

References 24 publications

Fourier ptychographic microscopy using wavelength multiplexing

Fourier ptychographic microscopy using wavelength multiplexing

Optimal illumination scheme for isotropic quantitative differential phase contrast microscopy

Acquisition of Multi-Modal Images of Structural Modifications in Glass with Programmable LED-Array-Based Illumination

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