Color Reactions of 1,10-Phenanthroline Derivatives

Moss, M. L.; Mellon, M. G.; Smith, G. Frederick

doi:10.1021/i560112a005

Cited by 47 publications

(7 citation statements)

References 8 publications

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“…Molar Absorptivity. The molar absorptivity of Fe(II)-phen3, calculated from Beer's law, was 1.106 X 104M-1 cm-1 (average of five determinations in the range of 2-5 X 10"5M Fe) and agreed well with the literature values (7).…”

Section: Resultssupporting

confidence: 88%

“…These 1,2-propanediol monoesters (1,2-monoesters) are usually prepared as a mixture of 1-and 2-monoesters accompanied with small amount of diester. Brandner reported that the equilibrium ratio of 1-to 2-monoester is dependent on the temperature (7). A variation in the 1-and 2-equilibrium ratio may cause a difference in performances.…”

Section: Discussionmentioning

confidence: 99%

“…

In a study (7) of the reaction between nitrilotriacetatoferrate (III) [Fe(III)NTA] and cysteine, a red-colored mixed complex was observed to form immediately on mixing and subsequently decompose. Investigation showed that the kinetics

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mentioning

confidence: 99%

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Spectrophotometric determination of cysteine

Bydalek¹,

Poldoski²

1968

Anal. Chem.

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Section: Resultssupporting

confidence: 88%

Section: Discussionmentioning

confidence: 99%

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Spectrophotometric determination of cysteine

Bydalek¹,

Poldoski²

1968

Anal. Chem.

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“…H202 concentrations were measured by UV absorption at 240 nm by assuming 8240 = 39.4 M-1 cm-1 (7) or by a peroxidase dye assay (8). Ferrous concentrations were determined by colorimetry with 1,10-phen by assuming that the Fe2+(1,10-phen)3 complex has an E510 = 1.11 X 104 M-1 cm-1 (9 (Fig. 1).…”

mentioning

confidence: 99%

Three chemically distinct types of oxidants formed by iron-mediated Fenton reactions in the presence of DNA.

Luo

Han

Chin

et al. 1994

Proc. Natl. Acad. Sci. U.S.A.

138

103

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Exposure of Escherichia coli to H202 leads to two kinetically distinguishable modes of killing: mode I killing occurs maximally near 2 mM H202, whereas mode II killing is essentially independent of H202 concentrations up to 20 mM.A major portion of H202 toxicity is attributed to DNA damage caused by the iron-mediated Fenton reaction. By studying DNA damage during Fenton reactions in vitro, the same complex kinetics were observed and three types of oxidants were distinguished based upon their reactivities toward H202 and alcohols and upon iron-chelator effects. but the unusual biphasic dose-response results to H202 observed for both killing and DNA nicking could indicate that there might be at least two types of oxidants. The objective of this study is to further investigate this possibility.MATERIALS AND METHODS H202 (30% solution) and 2-methyl-2-propanol ("t-butanol") were from Fisher Scientific. FeSO4, 1,10-phen, 4,7-phenanthroline (4,7-phen), 2,9-dimethyl-1,10-phenanthroline (dmphen), and 2,2'-dipy were from Aldrich. Ethanol was from Quantum Chemical (Tuscola, IL). Bacteriophage PM2 DNA labeled with [methyl-3H]thymidine (5) was isolated by detergent and phenol extraction, followed by density gradient centrifugation. The DNA was dialyzed against EDTA for at least 2 days and was not sensitive to H202 alone.Killing and Mutagenesis Analysis of E. coli. Strain AB1157 (WT) was exposed to H202 for 15 min and then plated. Where indicated, 1 mM 1,10-phen or 1 mM 2,2'-dipy was added to the cells 5 min prior to the challenge with H202.DNA Nicking Determination. PM2 [3H]DNA (17 AM nucleotide residues) was mixed with 0.8% NaCl/80 nM FeSO4 and 320 nM chelator ethanol and/or H202 as indicated. Reactions were stopped after 7.5 min at 25°C by the addition of catalase at 2 ,g/ml. Nicking of the DNA was quantitated by nitrocellulose filter binding as described (6).Other Methods. H202 concentrations were measured by UV absorption at 240 nm by assuming 8240 = 39.4 M-1 cm-1 (7) or by a peroxidase dye assay (8). Ferrous concentrations were determined by colorimetry with 1,10-phen by assuming that the Fe2+(1,10-phen)3 complex has an E510 = 1.11 X 104 M-1 cm-1 (9). The concentration of PM2 DNA was determined by UV absorption at 260 nm by assuming -260 = 6.5 mM-'-cm-'.RESULTS AND DISCUSSION Classification of Oxidants Formed by the Fenton Reaction in the Presence of DNA. In the presence of Fe2+ and H202, DNA nicking is maximal at 0.05 mM H202 then drops to about one-third that level at 3 mM H202, beyond which it remains relatively constant (Fig. 1). This dose-response result suggests that at least some species of the oxidant(s) is sensitive to H202.We were able to ascribe inhibition of aniline dye bleaching by higher concentrations of H202 to simple competition between H202 and the dye for -OH (4) in which H202 reacts with -OH to form the less active 'HO2 radical:H202 + 'OH -* 'HO2 + H20.[2]

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“…The 1, 10 phenanthroline method was employed to make orange-red complex (Moss and Mellon 1942). The standard stock solution containing 1000 mg L −1 of Fe 2+ was prepared by dissolving 6.02 g of iron (II) ammonium sulfate hexahydrate in 2 mL of 6 M hydrochloric acid (HCl), then diluted with 1 L of ultrapure water.…”

Section: Fementioning

confidence: 99%

Use of soil color meter for aqueous iron and ammonium measurements

Itai¹,

Maruoka²,

Kusakabe³

et al. 2013

Soil Science and Plant Nutrition

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In this paper, we proposed a new approach for on-site colorimetric analysis of ferrous ions (Fe 2+ ) and ammonium-nitrogen (NH 4 + -N) using a soil color meter as an alternative method to conventional spectrophotometry. The soil color meter we used can express solution color numerically on the basis of L*a*b* color space. After coloring of water by the 1, 10 phenanthroline method and the Indophenol blue method, the color of solution was measured by the soil color meter. , respectively. Since a similar apparatus is widely used in the soil science field, this technique would facilitate field surveys.

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Color Reactions of 1,10-Phenanthroline Derivatives

Cited by 47 publications

References 8 publications

Spectrophotometric determination of cysteine

Spectrophotometric determination of cysteine

Three chemically distinct types of oxidants formed by iron-mediated Fenton reactions in the presence of DNA.

Use of soil color meter for aqueous iron and ammonium measurements

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