Color test for 17-hydroxy, 20-keto, 21-methyl steroids

Properties of the ultraviolet-absorbing lipids produced by rat adrenals in vitro

“…A pale yellow was obtained with cortisol, cortolone and l1-deoxycorticosterone, a dark brown with corticosterone-21-aldehyde. Corticosterone, 1 1-dehydrocorticosterone, cortisone, progesterone and ll1hydroxyprogesterone remained colourless, whereas 17m-hydroxyprogesterone gave an orange colour, reported by McAleer & Kozlowski (1956) to be typical for 17-hydroxy-20-oxo-21-methylsteroids.…”

“…Other tests on paper included the Zimmermann test and the reaction for 17: 20-diols by oxidation with lead tetraacetate, followed by the Zimmermann test (Bush, 1955), the vanillin test (McAleer & Kozlowski, 1956), exposure to concentrated sulphuric acid and the Schiff test (Birmingham, 1959). In the last-named procedure the paper strips were passed through a 1 % solution of p-rosaniline hydrochloride in sulphurous acid.…”

Pharmaceuticals

“…Purification of urinary 17-oxosteroid extracts to yield products which give infrared spectra nearly identical with those of standards has been reported (60S). McAleer and Kozlowski (391) have developed a color test, specific for 17-hydroxy-a-keto-21-methyl steroids using vanillin and phosphoric acid to locate these compounds after paper chromatography. Analyses of mixtures of prednisone and cortisone by polarography at -0.90 volt in 50% methyl alcohol, buffered to pH 5.5 with sodium acetate-acetic acid, have been reported (312).…”

Chromogenic and fluorogenic reactions of adrenocortical and other steroids in concentrated acids

“…Cortisone, hydrocortisone (17-hydroxycorticosterone) and certain other adrenocortical steroids behaved similarly, although cortisone was said not to do so after chromatographic purification (Wintersteiner & Pfiffner, 1936;Reichstein & Shoppee, 1943). Similar colour or fluorescence phenomena have been reported from time to time with a large variety of steroids dissolved in different reagents such as concentrated phosphoric acid (Finkelstein, Hestrin & Koch, 1947;Boscott, 1948; Finkelstein, 1952), phthalic anhydride and zinc chloride (Garst, Nyc, Maron & Friedgood, 1950), perchloric acid (Tauber, 1952;Pontius, Beckmann & Voigt, 1955), anthrone in sulphuric acid (Graff, 1952), thiophen in sulphuric and acetic acids (Levine & Tao, 1953), diphenylamine in sulphuric and acetic acids (Clark, 1955), phosphorus pentoxide in sulphuric acid and vanillin in phosphoric acid (McAleer & Kozlowski, 1956).…”