Colorectal cancer stage evaluation using synchronous fluorescence spectroscopy technique

Genova, Tsanislava; Borisova, Ekaterina; Zhelyazkova, A.; Penkov, N; Vladimirov, Borislav; Terziev, Ivan; Semyachkina-Glushkovskaya, O.; Avramov, L.

doi:10.1007/s11082-016-0634-7

Cited by 17 publications

(7 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The reason for this spectral shift could be the high sensitivity of tryptophan's fluorescence to the environment such as pH and local electric field [19,34,36] Red-shift was also observed for the fluorescence maxima of tryptophan in breast cancer tissue's fluorescence [37]. The lower fluorescence intensity of collagen for cancerous tissues is its typical spectral hallmark [24,26,27,28] and in the presented results it is negligible low in comparison with collagen fluorescence maxima in healthy tissue spectra.…”

Section: Resultssupporting

confidence: 55%

“…In our previous works we have presented detailed investigations over the main fluorescence characteristics of gastrointestinal tissues, specific fluorophores and their significance for differentiation between cancerous and healthy GIT tissues [24][25][26][27]. We have also demonstrated implementation of synchronous fluorescence spectroscopy (SFS) [28] for tissue investigation and its superior to standard fluorescence spectroscopy, diagnostic potential [25][26][27].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Macro and micro spectroscopy parameters of cancerous and healthy gastrointestinal tissues

Genova

Borisova

Semyachkina-Glushkovskaya

et al. 2017

J-BPE

View full text Add to dashboard Cite

New more sensitive diagnostic modalities, for either analysis of biopsy tissue samples or on site, in vivo microscopy tissue examination, emerge to aid the detection and accurate diagnosis of gastrointestinal cancer. One of the most intensively investigated techniques is the fluorescence spectroscopy approach. We present our investigation of the detailed macro and micro fluorescence characteristics of ex-vivo fresh and fixed tissue samples. The fluorescence spectra of unprocessed cancerous and healthy gastrointestinal (GIT) tissue, obtained with two approaches fluorescence and synchronous fluorescence spectroscopy were compared with microspectroscopy performed with laser scanning confocal fluorescence microscopy (LSCFM) system in Lambda-scan regime over fixed tissue samples. The evaluated optical macro-and micro-spectroscopy characteristics are presented and disscused in order to improve the current understanding of the origin of autofluorescence contrast between healthy and cancerous tissues and its application for improvement of the existing diagnostic modalities.

show abstract

Section: Resultssupporting

confidence: 55%

Section: Introductionmentioning

confidence: 99%

Macro and micro spectroscopy parameters of cancerous and healthy gastrointestinal tissues

Genova

Borisova

Semyachkina-Glushkovskaya

et al. 2017

J-BPE

View full text Add to dashboard Cite

show abstract

“…For many years scientists have been comparing various colonic segments by different methods. Majority of this investigations have shown similarities for normal colonic mucosa as well as diseased one in distal colon and rectum and their difference from proximal colon [6][7][8][9][19][20][21][22]. It is also known [23], that pH is not equal in different parts of colon because of their morphological and functional peculiarities.…”

Section: The Results and Their Discussionmentioning

confidence: 99%

“…The most common method for CC investigation is registration of autofluorescence spectra in visible and near infrared region of spectrum. These results are described by several scientific groups . As an alternative, the registration of fluorescence excitation spectra could be measured.…”

Section: Introductionmentioning

confidence: 99%

Fluorescent diagnostics of epithelial neoplasms of different colon parts

2017

View full text Add to dashboard Cite

Biochemical changes and their similarities for precancer lesions and advanced colon cancer have described. Peculiarities of spectral data for different parts of colon may change the previous opinion about similar mechanisms of cancerogenesis for distal colon and rectum. Moreover, investigation of tissue specimen obtained for histological examination and containing lack of malignant epithelial cells in massive stroma does not interfere with analysis due to specific disproportion of spectrum maxima. Lasers Surg. Med. 49:763-766, 2017. © 2017 Wiley Periodicals, Inc.

show abstract

“…A detailed study on the endogenous fluorophores in the lower part of the gastrointestinal tract investigated with the fluorescence spectroscopy was carried out in the framework of this topic [40][41][42]. The implementation of a novel technique for tissue investigation -synchronous fluorescence spectroscopy, resulted in better addressing of the endogenous fluorophores observed in tissues [43][44][45][46]. The biological origin of the fluorescence contrast between cancerous and healthy gastrointestinal tissue was one of the main investigated areas [47], to determine more sensitive and specific optical markers for tissue differentiation and early detection of gastrointestinal cancer with autofluorescence diagnostics.…”

Section: Autofluorescence and Photodiagnosis Photodynamic Therapy Of Gastrointestinal Tractmentioning

confidence: 99%

A review of career devoted to Biophotonics – In memoriam to Ekaterina Borisova (1978–2021)

Genova¹,

Avramov²,

Kolev³

et al. 2021

J-BPE

View full text Add to dashboard Cite

Regretfully, because of her sudden demise, Assoc. Prof. Ekaterina Borisova is no longer amongst us. COVID-19 pulled away a brilliant scientist during the peak of her scientific career (see Fig. 1). All authors would like to express deepest condolences and sincere support to her family, friends, relatives and colleagues! We, therefore, rightfully commemorate her dedicated and devoted contribution to biophotonics, her readiness to always support, help, motivate and inspire all her colleagues and collaborators. Fig. 1 Assoc. Prof. Ekaterina Borisova examining samples under microscope.

show abstract

Colorectal cancer stage evaluation using synchronous fluorescence spectroscopy technique

Cited by 17 publications

References 32 publications

Macro and micro spectroscopy parameters of cancerous and healthy gastrointestinal tissues

Macro and micro spectroscopy parameters of cancerous and healthy gastrointestinal tissues

Fluorescent diagnostics of epithelial neoplasms of different colon parts

A review of career devoted to Biophotonics – In memoriam to Ekaterina Borisova (1978–2021)

Contact Info

Product

Resources

About