Colorectal cancer triple co-culture spheroid model to assess the biocompatibility and anticancer properties of polymeric nanoparticles

Bauleth‐Ramos, Tomás; Feijão, Tália; Gonçalves, André; Shahbazi, Mohammad‐Ali; Liu, Zehua; Barrias, Cristina C.; Oliveira, Maria José; Granja, Pedro L.; Santos, Hélder A.; Sarmento, Bruno

doi:10.1016/j.jconrel.2020.04.025

Cited by 51 publications

(59 citation statements)

References 91 publications

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“…For several cancer entities, the co-culture method can be enhanced by cultivating three cell types in one model [ 102 , 103 ]. The benefits of so-called triple-cultures apply for PDAC as well.…”

Section: Co-cultures Visualize Challenges Associated With Tumor–stmentioning

confidence: 99%

Three-Dimensional Cell Culture Systems in Radiopharmaceutical Cancer Research

Doctor

Seifert

Ullrich

et al. 2020

Cancers

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In preclinical cancer research, three-dimensional (3D) cell culture systems such as multicellular spheroids and organoids are becoming increasingly important. They provide valuable information before studies on animal models begin and, in some cases, are even suitable for reducing or replacing animal experiments. Furthermore, they recapitulate microtumors, metastases, and the tumor microenvironment much better than monolayer culture systems could. Three-dimensional models show higher structural complexity and diverse cell interactions while reflecting (patho)physiological phenomena such as oxygen and nutrient gradients in the course of their growth or development. These interactions and properties are of great importance for understanding the pathophysiological importance of stromal cells and the extracellular matrix for tumor progression, treatment response, or resistance mechanisms of solid tumors. Special emphasis is placed on co-cultivation with tumor-associated cells, which further increases the predictive value of 3D models, e.g., for drug development. The aim of this overview is to shed light on selected 3D models and their advantages and disadvantages, especially from the radiopharmacist’s point of view with focus on the suitability of 3D models for the radiopharmacological characterization of novel radiotracers and radiotherapeutics. Special attention is paid to pancreatic ductal adenocarcinoma (PDAC) as a predestined target for the development of new radionuclide-based theranostics.

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Section: Co-cultures Visualize Challenges Associated With Tumor–stmentioning

confidence: 99%

Three-Dimensional Cell Culture Systems in Radiopharmaceutical Cancer Research

Doctor

Seifert

Ullrich

et al. 2020

Cancers

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“…The complexity, heterogeneity, plasticity, and diversity of the human tumor microenvironment leaves models with inaccurate deductions regarding clinical responses. Although several interesting observations suggest that 3D co‐cultures are more relevant, these models are expensive, highly variable, and are therefore not suitable for large‐scale screening 101 …”

Section: Multicellular Modelsmentioning

confidence: 99%

Understanding cell‐cell communication and signaling in the colorectal cancer microenvironment

AlMusawi

Ahmed

Nateri

2021

Clinical & Translational Med

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Carcinomas are complex heterocellular systems containing epithelial cancer cells, stromal fibroblasts, and multiple immune cell‐types. Cell‐cell communication between these tumor microenvironments (TME) and cells drives cancer progression and influences response to existing therapies. In order to provide better treatments for patients, we must understand how various cell‐types collaborate within the TME to drive cancer and consider the multiple signals present between and within different cancer types. To investigate how tissues function, we need a model to measure both how signals are transferred between cells and how that information is processed within cells. The interplay of collaboration between different cell‐types requires cell‐cell communication. This article aims to review the current in vitro and in vivo mono‐cellular and multi‐cellular cultures models of colorectal cancer (CRC), and to explore how they can be used for single‐cell multi‐omics approaches for isolating multiple types of molecules from a single‐cell required for cell‐cell communication to distinguish cancer cells from normal cells. Integrating the existing single‐cell signaling measurements and models, and through understanding the cell identity and how different cell types communicate, will help predict drug sensitivities in tumor cells and between‐ and within‐patients responses.

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“…Various types of cocultured spheroids have been extensively reported to display enhanced imitation of physiological and pathological properties, by implementing a combination of cells necessary for various purposes. [ 4,31,32 ] These studies have introduced the coculturing of the parenchymal cells of a targeted organ with non‐parenchymal cells, such as fibroblasts, immune cells, and endothelial cells, to achieve morphological and functional benefits for modeling in vivo systems. Although multicellular spheroids that are morphologically similar to physiological tissues and organs have not yet been produced, spheroid formation presents the advantage of being one of the simplest available methods to form 3D microtissues of uniform size with different types of cells in favorable ratios.…”

Section: Strategies To Scale Up Number Of Dimensions Of Engineered Timentioning

confidence: 99%

Organ‐Level Functional 3D Tissue Constructs with Complex Compartments and their Preclinical Applications

et al. 2020

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There is an increasing interest in organ-level 3D tissue constructs, owing to their mirroring of in vivo-like features. This has resulted in a wide range of preclinical applications to obtain cell-or tissue-specific responses. Additionally, the development and improvement of sophisticated technologies, such as organoid generation, microfluidics, hydrogel engineering, and 3D printing, have enhanced 3D tissue constructs to become more elaborate. In particular, recent studies have focused on including complex compartments, i.e., vascular and innervation structured 3D tissue constructs, which mimic the nature of the human body in that all tissues/organs are interconnected and physiological phenomena are mediated through vascular and neural systems. Here, the strategies are categorized according to the number of dimensions (0D, 1D, 2D, and 3D) of the starting materials for scaling up, and novel approaches to introduce increased complexity in 3D tissue constructs are highlighted. Recent advances in preclinical applications are also investigated to gain insight into the future direction of 3D tissue construct research. Overcoming the challenges in improving organ-level functional 3D tissue constructs both in vitro and in vivo will ultimately become a life-saving tool in the biomedical field.

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Colorectal cancer triple co-culture spheroid model to assess the biocompatibility and anticancer properties of polymeric nanoparticles

Cited by 51 publications

References 91 publications

Three-Dimensional Cell Culture Systems in Radiopharmaceutical Cancer Research

Three-Dimensional Cell Culture Systems in Radiopharmaceutical Cancer Research

Understanding cell‐cell communication and signaling in the colorectal cancer microenvironment

Organ‐Level Functional 3D Tissue Constructs with Complex Compartments and their Preclinical Applications

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