Colorimetric allergenic fungal spore detection using peptide-modified gold nanoparticles

Lee, Byung Yang; Jang, Seok Cheon; Choi, Woong-Ku; Hong, Caleb; Ahn, Geum Ran; Kim, Seong Hwan; Chung, Woo‐Jae

doi:10.1016/j.snb.2020.128894

Cited by 55 publications

(24 citation statements)

References 42 publications

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“…A colorimetric method for the detection of Aspergillus niger spores was developed based on interactions between fungal spores and gold nanoparticles modified with a specific binding peptide that was identified by phage display screening. The specific binding peptides were able to detect 50 spores within 10 min [ 152 ]. As it stands, this will have limited clinical utility as fungi rarely spore in vivo; however, it could be adapted to detect hyphal wall components.…”

Section: Emerging Diagnostic Methodsmentioning

confidence: 99%

Biosensors and Diagnostics for Fungal Detection

Hussain

Malavia

Johnson

et al. 2020

JoF

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Early detection is critical to the successful treatment of life-threatening infections caused by fungal pathogens, as late diagnosis of systemic infection almost always equates with a poor prognosis. The field of fungal diagnostics has some tests that are relatively simple, rapid to perform and are potentially suitable at the point of care. However, there are also more complex high-technology methodologies that offer new opportunities regarding the scale and precision of fungal diagnosis, but may be more limited in their portability and affordability. Future developments in this field are increasingly incorporating new technologies provided by the use of new format biosensors. This overview provides a critical review of current fungal diagnostics and the development of new biophysical technologies that are being applied for selective new sensitive fungal biosensors to augment traditional diagnostic methodologies.

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Section: Emerging Diagnostic Methodsmentioning

confidence: 99%

Biosensors and Diagnostics for Fungal Detection

Hussain

Malavia

Johnson

et al. 2020

JoF

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“…Furthermore, the rapid and sensitive detection of fungal spores is of great interest due to the potential negative impacts on agriculture as well as public health. Lee et al, 2021, developed a technique for the detection of Aspergillus niger spore based on the specific peptides as a recognition probe and the AuNPs as the detection label [ 51 ]. The peptides enable rapid binding to the A. niger spore resulting in a visible change of colour intensity of the supernatant after sedimentation of the spores.…”

Section: Pathogen Biosensingmentioning

confidence: 99%

Gold Nanoparticles and Plant Pathogens: An Overview and Prospective for Biosensing in Forestry

Kulabhusan

Tripathi

Kant

2022

Sensors

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Plant diseases and their diagnoses are currently one of the global challenges and causes significant impact to the economy of farmers and industries depending on plant-based products. Plant pathogens such as viruses, bacteria, fungi, and pollution caused by the nanomaterial, as well other important elements of pollution, are the main reason for the loss of plants in agriculture and in forest ecosystems. Presently, various techniques are used to detect pathogens in trees, which includes DNA-based techniques, as well as other microscopy based identification and detection. However, these methodologies require complex instruments and time. Lately, nanomaterial-based new biosensing systems for early detection of diseases, with specificity and sensitivity, are developed and applied. This review highlights the nanomaterial-based biosensing methods of disease detection. Precise and time effective identification of plant pathogens will help to reduce losses in agriculture and forestry. This review focuses on various plant diseases and the requirements for a reliable, fast, and cost-effective testing method, as well as new biosensing technologies for the detection of diseases of field plants in forests at early stages of their growth.

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“…This result indicates binding between the ssDNA probe and cDNA, which results in a negatively charged surface leading to the attenuation of electron transfer. As a different type of transduction mechanism, colorimetric sensing mechanism has been utilized to detect biomolecules and viruses because of their simple visual readout and their capability to rapidly screen multiple analytes with high portability [176][177][178][179][180][181]. Several studies have been conducted to fabricate colorimetric biosensors using a paper substrate paired with a smartphone-based reader for application in POCT [182][183][184][185][186].…”

Section: Biosensorsmentioning

confidence: 99%

“…A paper/soluble polymer hybrid-based biosensing platform was developed for the diagnosis of myocardial infarction by detecting human cardiac troponin I (cTnI) as a standard biomarker [182]. Among the various biosensing platforms for POCT applications, lateral flow assays (LFAs) are the most widely used because of their major advantages such as As a different type of transduction mechanism, colorimetric sensing mechanism has been utilized to detect biomolecules and viruses because of their simple visual readout and their capability to rapidly screen multiple analytes with high portability [176][177][178][179][180][181]. Several studies have been conducted to fabricate colorimetric biosensors a paper substrate paired with a smartphone-based reader for application in POCT [182][183][184][185][186].…”

Section: Biosensorsmentioning

confidence: 99%

Nanomaterials for IoT Sensing Platforms and Point-of-Care Applications in South Korea

Choi

Lee

Choi

et al. 2022

Sensors

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Herein, state-of-the-art research advances in South Korea regarding the development of chemical sensing materials and fully integrated Internet of Things (IoT) sensing platforms were comprehensively reviewed for verifying the applicability of such sensing systems in point-of-care testing (POCT). Various organic/inorganic nanomaterials were synthesized and characterized to understand their fundamental chemical sensing mechanisms upon exposure to target analytes. Moreover, the applicability of nanomaterials integrated with IoT-based signal transducers for the real-time and on-site analysis of chemical species was verified. In this review, we focused on the development of noble nanostructures and signal transduction techniques for use in IoT sensing platforms, and based on their applications, such systems were classified into gas sensors, ion sensors, and biosensors. A future perspective for the development of chemical sensors was discussed for application to next-generation POCT systems that facilitate rapid and multiplexed screening of various analytes.

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Colorimetric allergenic fungal spore detection using peptide-modified gold nanoparticles

Cited by 55 publications

References 42 publications

Biosensors and Diagnostics for Fungal Detection

Biosensors and Diagnostics for Fungal Detection

Gold Nanoparticles and Plant Pathogens: An Overview and Prospective for Biosensing in Forestry

Nanomaterials for IoT Sensing Platforms and Point-of-Care Applications in South Korea

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