Colorimetric and chemiluminescence based enzyme linked apta-sorbent assay (ELASA) for ochratoxin A detection

Mukherjee, Monali; Nandhini, C.; Bhatt, Praveena

doi:10.1016/j.saa.2020.118875

Cited by 20 publications

(7 citation statements)

References 29 publications

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“…Mukherjee et al compared aptamer-based enzyme linked apta-sorbent assay (ELASA) with antibody-based ELISA and its potential to replace antibodies in usual immunoassay formats either as capture probe or detection probe without affecting the sensitivity [62]. The ELASA was based on the principle of target capture by aptamer where, OTA specific aptamer was used for toxin detection.…”

Section: Enzyme-linked Immunosorbent Assay (Elisa)mentioning

confidence: 99%

Advances in Colorimetric Strategies for Mycotoxins Detection: Toward Rapid Industrial Monitoring

Majdinasab

Aissa

Marty

2020

Toxins

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Mycotoxins contamination is a global public health concern. Therefore, highly sensitive and selective techniques are needed for their on-site monitoring. Several approaches are conceivable for mycotoxins analysis, among which colorimetric methods are the most attractive for commercialization purposes thanks to their visual read-out, easy operation, cost-effectiveness, and rapid response. This review covers the latest achievements in the last five years for the development of colorimetric methods specific to mycotoxins analysis, with a particular emphasis on their potential for large-scale applications in food industries. Gathering all types of (bio)receptors, main colorimetric methods are critically discussed, including enzyme-linked assays, lateral flow-assays, microfluidic devices, and homogenous in-solution strategies. This special focus on colorimetry as a versatile transduction method for mycotoxins analysis is comprehensively reviewed for the first time.

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Section: Enzyme-linked Immunosorbent Assay (Elisa)mentioning

confidence: 99%

Advances in Colorimetric Strategies for Mycotoxins Detection: Toward Rapid Industrial Monitoring

Majdinasab

Aissa

Marty

2020

Toxins

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“…Therefore, efficient distinguishment and elimination of multiple bacteria are an urgent need for early diagnosis and clinical treatment of infectious diseases, especially high-risk population. Conventional techniques for bacterial detection, including plating and culturing, polymerase chain reaction (PCR), , enzyme-linked immunosorbent assay (ELISA), , etc., are restricted by the shortcomings of high cost, long operation time, and complicated experimental procedures. Over the past two decades, the conventional “lock-and-key” detection mode has been developed for bacterial detection. − Although these methods have high sensitivity and good selectivity, most of them are only able to sense one specific bacteria .…”

Section: Introductionmentioning

confidence: 99%

Enhanced “Electronic Tongue” for Dental Bacterial Discrimination and Elimination Based on a DNA-Encoded Nanozyme Sensor Array

Zhang,

Qi,

Yang

et al. 2024

ACS Appl. Mater. Interfaces

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Bacterial infections are the second leading cause of death around the world, especially those caused by delayed treatment and misdiagnosis. Therefore, rapid discrimination and effective elimination of multiple bacteria are of great importance for improving the survival rate in clinic. Herein, a novel colorimetric sensor array for bacterial discrimination and elimination is constructed using programmable DNA-encoded iron oxide nanoparticles (IONPs) as sensing elements. Utilizing differential interactions of bacteria on DNA-encoded IONPs, 11 kinds of dental bacteria and 6 kinds of proteins have been successfully identified by linear discriminant analysis (LDA). Moreover, the developed sensing system also performs well in the quantitative determination of individual bacteria and identification of bacterial mixtures. More importantly, the practicability of this sensing strategy is further verified by precise differentiation of blind and artificial saliva samples. Furthermore, the sensor array is used for efficiently killing multiple bacteria, demonstrating great potential in clinical prophylaxis and therapy.

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“…For example, the European Commission proposed that the maximum limit for OTA in unprocessed cereals should not exceed 5 μg/kg (EC1881–2006). Traditional methods for OTA detection are mainly high-performance liquid chromatography (HPLC), enzyme-linked immunosorbent assays, , and other conventional methods . Although these methods have relatively high accuracy, they often require complex operation and expensive instrumentations.…”

Section: Introductionmentioning

confidence: 99%

Simple Design Concept for Dual-Channel Detection of Ochratoxin A Based on Bifunctional Metal–Organic Framework

Zhang

et al. 2022

ACS Appl. Mater. Interfaces

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A simple fluorescence and electrochemical dualchannel biosensor based on bifunctional Zr(IV)-based metal− organic framework (Zr-MOF) was proposed to detect Ochratoxin A (OTA). The bifunctional Zr-MOF, with photoluminescence properties and enormous electroactive ligands, was exploited to load OTA-specific aptamers for designing signal probes, greatly simplifying the probe-fabrication process and improving sensing reliability. Upon specific recognition of aptamer toward OTA, the anchored probe was released from the sensing interface into the reaction solution. In this circumstance, the increased amount of the signal probe in reaction solution led to an enhanced fluorescence response, while the decreased amount of the signal probe on the sensing interface resulted in a diminished electrochemical response. According to the dual-channel signal change with increasing OTA concentration, the visual fluorescence strategy was established for intuitive OTA detection, and meanwhile, sensitive electrochemical assay with a detection limit of 0.024 pg/mL was also achieved with the help of one-step electrodeposition as a sensing platform. Moreover, the proposed dual-channel assay has been successfully applied to determine OTA levels in corn samples with rapid response, superior accuracy, and high antiinterference capability, providing a promising method for food safety monitoring.

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Colorimetric and chemiluminescence based enzyme linked apta-sorbent assay (ELASA) for ochratoxin A detection

Cited by 20 publications

References 29 publications

Advances in Colorimetric Strategies for Mycotoxins Detection: Toward Rapid Industrial Monitoring

Advances in Colorimetric Strategies for Mycotoxins Detection: Toward Rapid Industrial Monitoring

Enhanced “Electronic Tongue” for Dental Bacterial Discrimination and Elimination Based on a DNA-Encoded Nanozyme Sensor Array

Simple Design Concept for Dual-Channel Detection of Ochratoxin A Based on Bifunctional Metal–Organic Framework

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