Colorimetric and Fluorescent Bimodal Ratiometric Probes for pH Sensing of Living Cells

Liu, Yuanyuan; Wu, Ming; Zhu, Lina; Feng, Xizeng; Kong, De‐Ming

doi:10.1002/asia.201500106

Cited by 33 publications

(17 citation statements)

References 39 publications

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“…Herein, the ratio of the RLS intensities at two wavelengths was used as signal output. Due to the self-referencing capability, such a ratiometric measurement modality can give high detection accuracy by reducing the effects of the fluctuation of some factors, including light source intensity, instrument sensitivity and probe concentration (22,48,49).…”

Section: Resultsmentioning

confidence: 99%

Cationic porphyrins with large side arm substituents as resonance light scattering ratiometric probes for specific recognition of nucleic acid G-quadruplexes

Zhang

Cui

Zhu

et al. 2019

Nucleic Acids Research

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Specific G-quadruplex-probing is crucial for both biological sciences and biosensing applications. Most reported probes are focused on fluorescent or colorimetric recognition of G-quadruplexes. Herein, for the first time, we reported a new specific G-quadruplex-probing technique—resonance light scattering (RLS)-based ratiometric recognition. To achieve the RLS probing of G-quadruplexes in the important physiological pH range of 7.4-6.0, four water soluble cationic porphyrin derivatives, including an unreported octa-cationic porphyrin, with large side arm substituents were synthesized and developed as RLS probes. These RLS probes were demonstrated to work well for ratiometric recognition of G-quadruplexes with high specificity against single- and double-stranded DNAs, including long double-stranded ones. The working mechanism was speculated to be based on the RLS signal changes caused by porphyrin protonation that was promoted by the end-stacking of porphyrins on G-quadruplexes. This work adds an important member in G-quadruplex probe family, thus providing a useful tool for studies on G-quadruplex-related events concerning G-quadruplex formation, destruction and changes in size, shape and aggregation. As a proof-of-concept example of applications, the RLS probes were demonstrated to work well for label-free and sequence-specific sensing of microRNA. This work also provides a simple and useful way for the preparation of cationic porphyrins with high charges.

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Section: Resultsmentioning

confidence: 99%

Cationic porphyrins with large side arm substituents as resonance light scattering ratiometric probes for specific recognition of nucleic acid G-quadruplexes

Zhang

Cui

Zhu

et al. 2019

Nucleic Acids Research

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“…Liu et al . have synthesized porphyrin derivatives for ratiometric fluorescent pH sensors having intensity ratios at 656/719 nm to obtain sigmoidal relationship at an excitation wavelength of 446 nm 29 . Other fluorescent ratiometric biosensors developed by Niu et al .…”

Section: Introductionmentioning

confidence: 99%

pH and Urea Estimation in Urine Samples using Single Fluorophore and Ratiometric Fluorescent Biosensors

Chaudhari

Joshi

Srivastava

2017

Sci Rep

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Kidney diseases remain often undiagnosed due to inefficient screening methods available at patient’s disposal. Early diagnosis and effective management of kidney problems can best be addressed by the development of biosensors for commonly occurring clinical biomarkers. Here we report the development of single fluorophore and dual fluorophore ratiometric biosensors based on alginate microspheres for pH and urea analysis in urine samples. A facile method of air driven atomization was used for developing these polymeric fluorophore and enzyme based biosensors. Ratiometric biosensors were developed using layer-by-layer coating of polyelectrolyte conjugated to reference fluorophores. Biosensing studies using these biosensors showed that samples in pathophysiological range can be measured having pH range of 4–8 and urea levels between 0–50 mM. Testing of urine samples using these biosensors showed that both pH and urea detection can be accurately performed without interference. Thus, we believe that FITC-Dextran and FITC-Dextran/RuBpy based pH and urea biosensors show a great potential to be translated as a point of care device for pH and urea biosensing in early detection and continuous monitoring of kidney diseases.

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“…To date, fluorescence spectroscopy has been extensively used for the measurement of pH due to its noninvasiveness, high selectivity and sensitivity, rapid response time, and ease of operation . As a result, a number of fluorescent pH probes have been reported in the literature, functioning mainly at three pH regions: (1) cytosolic pH at 6.8–7.4, (2) lysosomal and endosomal pH at 4.5–6.0, and (3) mitochondrial pH at ~8.0 .…”

Section: Introductionmentioning

confidence: 99%

A Colorimetric and Fluorescent pH Probe for Extremely Acidic Conditions and its Application in pH Test Paper

You¹,

Shen²,

Shen³

et al. 2018

Bulletin Korean Chem Soc

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Here, we report a highly water‐soluble colorimetric and fluorescent pH probe that works in the pH range 0.2–3.0. The probe showed a rapid response, high stability, and excellent reversibility to acidity. Moreover, the fluorescence of probe was not influenced by the existence of high concentration of cations. The pKa of probe was calculated to be 2.25 ± 0.06. Furthermore, the pH test papers coated with the probe exhibited a distinct color change in acidic conditions.

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Colorimetric and Fluorescent Bimodal Ratiometric Probes for pH Sensing of Living Cells

Cited by 33 publications

References 39 publications

Cationic porphyrins with large side arm substituents as resonance light scattering ratiometric probes for specific recognition of nucleic acid G-quadruplexes

Cationic porphyrins with large side arm substituents as resonance light scattering ratiometric probes for specific recognition of nucleic acid G-quadruplexes

pH and Urea Estimation in Urine Samples using Single Fluorophore and Ratiometric Fluorescent Biosensors

A Colorimetric and Fluorescent pH Probe for Extremely Acidic Conditions and its Application in pH Test Paper

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