Colorimetric and Fluorimetric Determination of Steroids

Bartoš, Jaroslav; Pesez, Maurice

doi:10.1515/iupac.51.0507

Cited by 7 publications

(8 citation statements)

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“…Corticosterone level was estimated as per the procedure discovered by Bartos et al (1979) using Ultra violet -visible spectrophotometer (Thermo Scientific Genesys TM 180, USA). 29 Biochemical assessments in brain As followed earlier in our laboratory, "After collecting blood samples on 23 rd day from the mice of groups 1-10, the brain of these mice were separated. The brain samples were washed in cold 0.25 M sucrose -0.1 M Tris-0.02 M EDTA buffer having pH 7.4.…”

Section: Assessment Of Plasma Corticosteronementioning

confidence: 99%

Pharmacological Screening of Gentisic acid for Antidepressant Activity in Unstressed and Stressed Mice

Bansal

Dhingra

2022

IJPI

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Symptoms of sad mood, hopelessness, loss of interest and pleasure, insomnia, feeling of worthlessness and suicidal thoughts indicate depression. 1 It affects more than 280 million people worldwide. 2 Depression may be due to deficiency of monoamine neurotransmitters, dysregulation of HPA (hypothalamic-pituitary-adrenal axis), neuroinflammation and oxidative stress. [3][4][5][6] Continued exposure to stress increases oxidative stress and produced neuroinflammation in brain of humans; which may lead to depression. 7 Chronic unpredictable mild stress (CUMS) is commonly employed model to induce depression in laboratory animals. 8 CUMS leads to anhedonia, increased anxiety-like behavior, and hyperactivity of HPA axis in rodents. 9 The conventional antidepressants presently used to treat depression in allopathic system of medicine have many side effects like body weight gain, sexual dysfunction, sedation, fatigue, etc. 10 Some plants like Hypericum perforatum, Melissa officinalis; bioactive compounds such as crocin, and glycyrrhizic acid, etc. have been reported to alleviate depression in clinical trials. 11-14 Thus, there is strong need for discovering plant-based antidepressants having equal effectiveness to Allopathic medications with no or less side effects. 15 Gentisic acid, also known as 2,5-dihydroxybenzoic acid, is found in citrus fruits (Citrus species), Gentiana species, Vitis vinifera, red sandal wood (Pterocarpus santalinus), Hibiscus rosa-sinensis, Olea europaea, Sesamum indicum, Madagascar rosy periwinkle, avocados, kiwi and apples. [16][17] Gentisic acid has anti-Alzheimer, anti-Parkinsonian, antioxidant, antiinflammatory and cardioprotective properties. [18][19][20][21][22] Since gentisic acid possesses antioxidant and neuroprotective activities, so the current research was intended to study effect of gentisic acid on depressive behavior in normal mice and also stressed mice. MATERIALS AND METHODS AnimalsSwiss albino male mice (28-32 g, 2-3 months old) were used in the current research study. Since estrogens possesses antidepressant activity, that is why female mice were excluded. 23 The animals were kept in cages (n = 8 each) in an air-conditioned room (temperature 24-25°C) with twelve hours light and twelve hours dark cycle. The number of mice to be used for research study were approved by Institutional Animals Ethics Committee in the meeting held on March 4, 2020 (IAEC Minutes letter No. IAEC/2020/10-18, dated 04 th March, 2020). Drugs and chemicalsGentisic acid was procured from Hi-media Laboratories Pvt. Ltd. (Mumbai). It was suspended in 1% w/v carboxy methyl cellulose (CMC). Fluoxetine was purchased from Sigma-Aldrich, St Louis, USA. Fluoxetine was dissolved in normal saline. Other chemicals employed were of analytical grade. CUMS procedureAccording to Willner et al. (1992) and as followed earlier in our laboratory, "Mice were given variable sequence of following mild unpredictable stressors once a day between 10:30 and 16:00 hr for 21 days:

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Section: Assessment Of Plasma Corticosteronementioning

confidence: 99%

Pharmacological Screening of Gentisic acid for Antidepressant Activity in Unstressed and Stressed Mice

Bansal

Dhingra

2022

IJPI

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“…On the 15 th day, after completion of the test session of forced swim test blood samples were collected by retro-orbital plexuses and centrifuged (Remi Centrifuge, Mumbai, India) at 2500 rpm for 10 min to separate plasma for measurement of corticosterone 15 . The animals were sacrificed by cervical dislocation, and immediately brain was removed for measurement of monoamines (5HT, DA, and NE) and γ aminobutyric acid (GABA).…”

Section: Biochemical Estimationsmentioning

confidence: 99%

“…respectively for 15 consecutive days. On the 15 th day, after completion of forced swim test, blood samples were collected by retro-orbital plexus and centrifuged (Remi Centrifuge, Mumbai, India) at 2500 rpm for 10 min to separate plasma for measurement of corticosterone 15 . To 1 ml of plasma sample, 1.0 ml of ethanol, 0.5 ml of 0.1% solution of p-nitroso-N, N-dimethyl aniline in ethanol was added.…”

Section: Estimation Of Plasma Corticosteronementioning

confidence: 99%

Untitled

2019

IJPSR

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The active ingredient flavonoids especially Flavones, e.g., Quercetin was identified from the methanol extract of Alpinia officinarum. This study evaluates the antidepressant activity of Alpinia officinarum (L.) wild Hance (Zingiberaceae) in Swiss Albino mice models. The antidepressant activity was evaluated by using different animal models viz. tail suspension test, despair swim test, potentiation of norepinephrine toxicity test, 5-hydroxytryptophan potentiation in Swiss Albino mice. The methanol extract of Alpinia officinarum significantly increases Na + , K + -ATPase level in the brain in the tail suspension test model. An antidepressant effect in the despair swim test was confirmed by the increase in monoamines (NE, DA, 5HT) and γ-aminobutyric acid levels in the brain. Alpinia officinarum methanol extract also contributes to the normal regulation of the HPA axis by significant (P<0.001) decreased in plasma corticosterone level. Alpinia officinarum (400 mg/kg) showed antidepressant potential by increasing the number of head twitches induced by 5 HTP. Antidepressant effect of an extract of Alpinia officinarum at a dose of 400 mg/kg could be via modulation of brain monoamines and normalizing hypothalamic pituitary adrenal axis. QUICK RESPONSE CODE

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“…The quantitative estimation of corticosterone levels in plasma was performed as per the reported method [35].…”

Section: Estimation Of Plasma Corticosterone Levelsmentioning

confidence: 99%

Antidepressant-Like Activity of Flowers of Tecomella Undulata in Mice Subjected to Chronic Unpredictable Mild Stress

Dhingra

Deepak

2019

Asian J Pharm Clin Res

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Objective: Flowers of Tecomella undulata have been reported to be a rich source of flavonoids such as rutin and quercetin. The present study was designed to evaluate the effect of ethanol extract of flowers of T. undulata on chronic unpredictable mild stress (CUMS)-induced depression in Swiss young male albino mice.Methods: The mice were subjected to CUMS for 21 successive days. Ethanol extract of the flowers (50, 100, and 200 mg/kg, p.o.) and fluoxetine (20 mg/kg, p.o.) per se was administered for 21 successive days to separate groups of unstressed and stressed mice. Tail suspension test (TST) and sucrose preference test were used to evaluate the effect of the extract on depression-like behavior in mice.Results: Extract of flowers of T. undulata (100 and 200 mg/kg) significantly decreased immobility period of stressed mice in TST, indicating significant antidepressant-like activity of the extract. Stress-induced reduced sucrose preference was significantly restored by the extract. There was no significant effect on locomotor activity of mice by the extract and fluoxetine. The extract significantly reversed stress-induced increase in brain malondialdehyde levels; plasma nitrite and corticosterone levels; and also significantly reversed the stress-induced decrease in reduced glutathione and catalase levels. There was no significant effect of the extract on brain MAO-A activity in both unstressed and stressed mice.Conclusion: These results indicated that ethanol extract of flowers of T. undulata showed significant antidepressant-like activity in mice subjected to CUMS, probably through alleviation of oxidative stress and decrease in plasma corticosterone levels.

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Colorimetric and Fluorimetric Determination of Steroids

Cited by 7 publications

References 0 publications

Pharmacological Screening of Gentisic acid for Antidepressant Activity in Unstressed and Stressed Mice

Pharmacological Screening of Gentisic acid for Antidepressant Activity in Unstressed and Stressed Mice

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Antidepressant-Like Activity of Flowers of Tecomella Undulata in Mice Subjected to Chronic Unpredictable Mild Stress

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