Colorimetric and photothermal immunosensor for sensitive detection of cancer biomarkers based on enzyme-mediated growth of gold nanostars on polydopamine

Gao, Yuting; Wu, Yan; Huang, Pengcheng; Wu, Fang-Ying

doi:10.1016/j.aca.2023.341775

Cited by 12 publications

(2 citation statements)

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“…3,4 Consequently, the development and implementation of high-sensitivity PSA detection methods have emerged as a focal point in the realm of prostate cancer prevention and treatment. 5−8 To improve the precision and dependability of PSA detection, novel techniques and methods were explored, such as an enzyme-linked immunosorbent assay (ELISA) 9 a field transistor biosensor, 10,11 a quartz crystal microbalance, 12 and microfluidic technology. 13 Among them, widely reported ELISA was recognized by researchers for its high sensitivity and quantification capabilities.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Prostate cancer poses a grave threat to male health, accounting for a staggering 26% of male malignant tumor incidence rates worldwide, ranking at the forefront. , Prostate-specific antigen (PSA), serving as a diagnostic marker for prostate cancer, is widely employed to indicate the onset and progression stages of the disease. , Consequently, the development and implementation of high-sensitivity PSA detection methods have emerged as a focal point in the realm of prostate cancer prevention and treatment. − To improve the precision and dependability of PSA detection, novel techniques and methods were explored, such as an enzyme-linked immunosorbent assay (ELISA) a field transistor biosensor, , a quartz crystal microbalance, and microfluidic technology . Among them, widely reported ELISA was recognized by researchers for its high sensitivity and quantification capabilities. ,, However, reliable PSA results through ELISA require specialized equipment, trained personnel, and a consistent laboratory environment. ,, Accordingly, the application of ELISA in point-of-care testing (POCT) is hindered by the extended detection time, demanding requirements, and complex operation. − …”

Section: Introductionmentioning

confidence: 99%

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Colorimetric and Photothermal Dual-Modal Switching Lateral Flow Immunoassay Based on a Forced Dispersion Prussian Blue Nanocomposite for the Sensitive Detection of Prostate-Specific Antigen

Gong,

Gai,

Tao

et al. 2024

Anal. Chem.

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Prostate-specific antigen (PSA) is a key marker for a prostate cancer diagnosis. The low sensitivity of traditional lateral flow immunoassay (LFIA) methods makes them unsuitable for point-of-care testing. Herein, we designed a nanozyme by in situ growth of Prussian blue (PB) within the pores of dendritic mesoporous silica (DMSN). The PB was forcibly dispersed into the pores of DMSN, leading to an increase in exposed active sites. Consequently, the atom utilization is enhanced, resulting in superior peroxidase (POD)-like activity compared to that of cubic PB. Antibody-modified DMSN@PB nanozymes serve as immunological probes in an enzymatic-enhanced colorimetric and photothermal dual-signal LFIA for PSA detection. After systematic optimization, the LFIA based on DMSN@PB successfully achieves a 4-fold amplification of the colorimetric signal within 7 min through catalytic oxidation of the chromogenic substrate by POD-like activity. Moreover, DMSN@PB exhibits an excellent photothermal conversion ability under 808 nm laser irradiation. Accordingly, photothermal signals are introduced to improve the anti-interference ability and sensitivity of LFIA, exhibiting a wide linear range (1–40 ng mL–1) and a low PSA detection limit (0.202 ng mL–1), which satisfies the early detection level of prostate cancer. This research provides a more accurate and reliable visualization analysis methodology for the early diagnosis of prostate cancer.

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