Colorimetric assay of ethanol using alcohol dehydrogenase from dry baker's yeast

Zanon, Juliana P.; Peres, Maristela F.S.; Gattás, Edwil Aparecida de Lucca

doi:10.1016/j.enzmictec.2006.07.029

Cited by 47 publications

(33 citation statements)

References 18 publications

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“…Thus, YADH is potentially useful for producing optically active alcohols under physiological conditions, which are of significance in the chemical, pharmaceutical and cosmetic industries. Furthermore, YADH was utilized in the reagentless electrochemical biosensors for alcohol detection with high sensitivity (Williams & Hupp 1998;Zanon et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Expression of soluble Saccharomyces cerevisiae alcohol dehydrogenase in Escherichia coli applicable to oxido-reduction bioconversions

et al. 2014

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Six-carbon aldehydes and alcohols belong to flavours and fragrances with wide application in the food, feed, cosmetic, chemical and pharmaceutical sectors. In the present study, we prepared the expression system for production of recombinant yeast alcohol dehydrogenase 1 (YADH1) from Saccharomyces cerevisiae which is suitable also for catalysis of the interconversion of C-6 aldehydes and alcohols. We have demonstrated that an effective three-step strategy can overcome the insolubility problems during YADH1 production in Escherichia coli. We used trxB and gor deficient expression strain, decreased concentration of isopropyl β-D-1-thiogalactopyranoside and lowered temperature to 20• C during induction. Finally, kinetic parameters of recombinant YADH1 were determined and we concluded it is a promising enzyme also for the interconversion of C-6 alcohols/aldehydes in green note volatile production.

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Section: Introductionmentioning

confidence: 99%

Expression of soluble Saccharomyces cerevisiae alcohol dehydrogenase in Escherichia coli applicable to oxido-reduction bioconversions

et al. 2014

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“…One of the most used enzymatic method for the determination of ethanol is based on the oxidation of ethanol to acetaldehyde by alcohol dehydrogenase (ADH) (http://www.sigmaaldrich.com/content/dam/sigmaaldrich/docs/Sigma/Bulletin/1/mak076bul.pdf; http://www.worthingtonbiochem.com/ADH/assay.html; Zanon et al, 2007). This reaction produces NADH, which is determined photometrically.…”

Section: Ethanol Methanol and Formaldehyde Analyzing With The Use Ofmentioning

confidence: 99%

The Use of Enzymes for Ethanol, Methanol and Formaldehyde Determination in Food Products

Sibirnyj¹,

Grabek-Lejko²,

Gonchar³

2015

J microb biotech food sci

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There is a need for the development and the use of analytical methods for assaying ethanol, methanol and formaldehyde in foods. Ethanol is a major component of a variety of alcoholic beverages and is an ingredient of a number of foodstuffs. Formaldehyde and methanol are produced on the large scale, are very toxic and have mutagenic and carcinogenic action on living organisms. The presence of toxic methanol and formaldehyde in foods can lead to severe poisoning and even death. Many current methods for the determination of ethanol, methanol and formaldehyde are based on the use of enzymes. The article presents an overview of the classical enzymatic and biosensors’ methods for the analysis of these compounds.

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“…For ethanol quantification, the ethanol determination kit (Sigma-Aldrich) was used, according to the methodology described by Zanon et al (2006) [24], at 340 nm.…”

Section: Determination Of the Consumption Of Carbon Source And Ethanomentioning

confidence: 99%

Proteome and fermentative parameters of Saccharomyces cerevisiae CAT-1 under Very High Gravity Fermentation (VHGF) using sugarcane juice

2017

J App Biol Biotech

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Alcoholic fermentation is an important process in the modern world, allowing the production of ethanol for several applications. Different Saccharomyces cerevisiae strains have been used for this purpose, such as CAT-1, a strain resistant to different stress factors. Hence, our aim was to analyze some fermentative parameters and the proteome of S. cerevisae CAT-1 under Very High Gravity Fermentation (VHGF) using sugarcane juice as fermentative medium. The yeast was cultured in the must with the sucrose concentration adjusted to 2%, 14%, 21% and 30%, for 10 h at 30 ºC. The cell viability was 96-100% for all sucrose concentrations analyzed and the biomass increased for each condition as time function. The highest ethanol recovery was obtained under 30% sucrose. Considering the S. cerevisiae CAT-1 proteome under 14% and 30% sucrose, qualitative and quantitative differences were found in the protein expression. Important enzymes for fermentation, such as enolase and one alcohol dehydrogenase isoform were more expressed at 30% sucrose than with 14% sucrose. The yeast S. cerevisiae CAT-1 is an interesting strain to be used for fermentation under VHGF technology using sugarcane juice, allowing high ethanol recovery with increased expression of proteins related to alcoholic fermentation and viability as well.

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Colorimetric assay of ethanol using alcohol dehydrogenase from dry baker's yeast

Cited by 47 publications

References 18 publications

Expression of soluble Saccharomyces cerevisiae alcohol dehydrogenase in Escherichia coli applicable to oxido-reduction bioconversions

Expression of soluble Saccharomyces cerevisiae alcohol dehydrogenase in Escherichia coli applicable to oxido-reduction bioconversions

The Use of Enzymes for Ethanol, Methanol and Formaldehyde Determination in Food Products

Proteome and fermentative parameters of Saccharomyces cerevisiae CAT-1 under Very High Gravity Fermentation (VHGF) using sugarcane juice

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