Colorimetric assay of homocysteine using gold nanoparticles conjugated with thermoresponsive copolymers

Uehara, Nobuo

doi:10.1039/c6ay02002j

Cited by 6 publications

(5 citation statements)

References 59 publications

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“…The standard illuminant (D65) defined by the International Commission on Illumination (CIE) was selected to simulate average daylight [ 33 ]. The standard CIE L*a*b* coordinates [ 34 , 35 , 36 ], as shown in Figure 6 , were adopted and average values recorded for each sample as well as the standard deviations.…”

Section: Methodsmentioning

confidence: 99%

Quantification and Variability Analysis of Lignin Optical Properties for Colour-Dependent Industrial Applications

et al. 2018

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Lignin availability has increased significantly due to the commercialization of several processes for recovery and further development of alternatives for integration into Kraft pulp mills. Also, progress in lignin characterization, understanding of its chemistry as well as processing methods have resulted in the identification of novel lignin-based products and potential derivatives, which can serve as building block chemicals. However, all these have not led to the successful commercialization of lignin-based chemicals and materials. This is because most analyses and characterizations focus only on the technical suitability and quantify only the composition, functional groups present, size and morphology. Optical properties, such as the colour, which influences the uptake by users for diverse applications, are neither taken into consideration nor analysed. This paper investigates the quantification of lignin optical properties and how they can be influenced by process operating conditions. Lignin extraction conditions were also successfully correlated to the powder colour. About 120 lignin samples were collected and the variability of their colours quantified with the CIE L*a*b* colour space. In addition, a robust and reproducible colour measurement method was developed. This work lays the foundation for identifying chromophore molecules in lignin, as a step towards correlating the colour to the functional groups and the purity.

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Section: Methodsmentioning

confidence: 99%

Quantification and Variability Analysis of Lignin Optical Properties for Colour-Dependent Industrial Applications

et al. 2018

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“…have different melting temperatures, and therefore they can be distinguished within the assay by AuNMs disassociation based on temperature [166][167][168]. Similarly, a thermoresponsive AuNMs biosensor capable of distinguishing between homocysteine and cysteine has been reported [169]. The assay used AuNMs conjugated with thermoresponsive copolymers.…”

Section: Au Nano Vesiclementioning

confidence: 99%

Latest advances in combining gold nanomaterials with physical stimuli towards new responsive therapeutic and diagnostic strategies

Movia

Benhaddada

Spadavecchia

et al. 2020

Precision Nanomedicine

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Graphical abstract KeywordsGold nanoparticle 1 ; external stimuli; physical stimuli; physically triggered nanomedicine. Quote this article as: Movia D, Benhaddada M, Jolanda Spadavecchia J, Prina-Mello A, Latest advances in combining gold nanomaterials with physical stimuli towards new responsive therapeutic and diagnostic strategies, Precis. Nanomed. 2020 April;3(2):495-524, https://doi. AbstractNanomedicine aims at enhancing treatment efficiency and/or improving diagnostic sensitivity by better controlling several critical parameters, such as tissue targeting and off-target toxicity. More recently, advanced nanomedicine products have been developed to achieve spatially and temporally controlled therapy and diagnosis. This review focuses on gold nanomaterials (AuNMs) and alloy/hybrid AuNMs that can be used in stimuli-responsive strategies for therapeutic and diagnostic applications. Endogenous and/or exogenous stimuli can be used as a trigger for such systems. Herein, we focus on those activated by exogenous stimuli. Our review starts from one specific externally activated product, Aurolase®, which recently underwent clinical studies. Further we continue describing a specific physically triggered category, for which the exogeneous stimulus applied induces a structural transformation or modification that is essential for their therapeutic and/or diagnostic action. Gold nanomaterials are grouped both by the nature of the function they exert (therapeutic or diagnostic) and the stimuli class.

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“…Leesutthiphonchai et al 21 reported the selective determination of Hcy levels in human plasma using a silver nanoparticle-based colorimetric assay. Uehara reported 22 the colorimetric assay of Hcy using gold nanoparticles conjugated with thermoresponsive copolymers. Thus, there are very few reports on the colorimetric sensing of Hcy using functionalized metallic nanoparticles.…”

Section: Introductionmentioning

confidence: 99%

Synthesis and Characterization of 1H-Imidazole-4,5-dicarboxylic Acid-Functionalized Silver Nanoparticles: Dual Colorimetric Sensors of Zn²⁺ and Homocysteine

Mondal

Yarger

2022

ACS Omega

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A colorimetric assay has been developed for Zn 2+ and homocysteine (Hcy) detection using functionalized silver nanoparticles (AgNPs). AgNPs have been synthesized using silver nitrate, where sodium citrate is used as a stabilizing agent and NaBH 4 as a reducing agent. Then, the nanoparticles (citrate@AgNPs) were functionalized with 1 H -imidazole-4,5-dicarboxylic acid (IDCA). UV–visible and FTIR spectra suggested that IDCA was functionalized on the surface of citrate@AgNPs through the N atom of the imidazole ring. The IDCA-functionalized silver nanoparticles (IDCA@AgNPs) simultaneously detected Zn 2+ and Hcy from aqueous solution and showed different responses to the two analytes (Zn 2+ and Hcy) based on the aggregation-induced color change of IDCA@AgNPs. They showed the color change from yellow to red, which was easily discriminated by visual inspection as well as UV–visible spectroscopy. The surface plasmon resonance absorbance values of Zn 2+ and Hcy are 485 and 512 nm, respectively, when Zn 2+ and Hcy react with IDCA@AgNPs. IDCA@AgNPs showed linearity with Zn 2+ and Hcy concentrations, with the detection limit of 2.38 μM and 0.54 nM, respectively (S/N = 3). The IDCA@AgNPs showed excellent selectivity toward Zn 2+ and Hcy compared to the different metal ions and amino acids, respectively. Optimal detection was achieved toward Zn 2+ and Hcy in the pH range 3–10. In addition, IDCA@AgNPs were used to detect Zn 2+ and Hcy from lake water, showing low interference.

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Colorimetric assay of homocysteine using gold nanoparticles conjugated with thermoresponsive copolymers

Abstract: Homocysteine was distinguished from cysteine and assayed by colorimetry with gold nanocomposites composed of 13 nm gold nanoparticles conjugated with thermoresponsive copolymers, poly(N-isopropylacrylamide-co-2-(dimethylamino)-ethylmethacrylate).

Cited by 6 publications

References 59 publications

Quantification and Variability Analysis of Lignin Optical Properties for Colour-Dependent Industrial Applications

Quantification and Variability Analysis of Lignin Optical Properties for Colour-Dependent Industrial Applications

Latest advances in combining gold nanomaterials with physical stimuli towards new responsive therapeutic and diagnostic strategies

Synthesis and Characterization of 1H-Imidazole-4,5-dicarboxylic Acid-Functionalized Silver Nanoparticles: Dual Colorimetric Sensors of Zn²⁺ and Homocysteine

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Colorimetric assay of homocysteine using gold nanoparticles conjugated with thermoresponsive copolymers

Abstract: Homocysteine was distinguished from cysteine and assayed by colorimetry with gold nanocomposites composed of 13 nm gold nanoparticles conjugated with thermoresponsive copolymers, poly(N-isopropylacrylamide-co-2-(dimethylamino)-ethylmethacrylate).

Cited by 6 publications

References 59 publications

Quantification and Variability Analysis of Lignin Optical Properties for Colour-Dependent Industrial Applications

Quantification and Variability Analysis of Lignin Optical Properties for Colour-Dependent Industrial Applications

Latest advances in combining gold nanomaterials with physical stimuli towards new responsive therapeutic and diagnostic strategies

Synthesis and Characterization of 1H-Imidazole-4,5-dicarboxylic Acid-Functionalized Silver Nanoparticles: Dual Colorimetric Sensors of Zn2+ and Homocysteine

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Synthesis and Characterization of 1H-Imidazole-4,5-dicarboxylic Acid-Functionalized Silver Nanoparticles: Dual Colorimetric Sensors of Zn²⁺ and Homocysteine