Colorimetric-assisted photoelectrochemical sensing for dual-model detection of sialic acid via oxidation-power mediator integration

Construction of Multi-Mode Photoelectrochemical Immunoassays for Accurate Detection of Cancer Markers: Assisted with MOF-Confined Plasmonic Nanozyme

“…20−22 The response signals triggered by target synchronization are relatively independent and could be mutually cross-checked, which effectively reduces false positive/negative errors and improves the reliability of clinical diagnosis. 23,24 Since the initial exploration of PEC-involved dual-mode detection by Zhao, Chen and co-workers, many multimode sensing methods have been developed in biomolecule detection, including PEC-fluorescence, 25,26 PECelectrochemistry, 27,28 PEC-colorimetry 29,30 and PEC-photothermal imaging. 22,31 Despite the superiority of these studies, the PEC involved multimode analysis, which remains at an early stage of development.…”

“…Photoelectrochemical bioanalysis, as a recently emerged and rapidly advancing analytical technology, has garnered significant research attention due to its inherent advantages of high sensitivity, low background, handy operation, and easy miniaturization. − By skillfully designing photoactive materials and signal amplification strategies, many innovative PEC methods have been developed for the ultrasensitive detection of disease markers. − However, the single PEC signal readout mode is vulnerable to variable coexisting interferents such as an unstable experimental environment, nonstandard operation procedures, and altered handling personnel, resulting in reduced information acquisition, poor accuracy and reliability in practical applications. − Compared with single-mode PEC bioanalysis, PEC-involved multimode immunoassays, combining the individual advantages of each mode (e.g., electrochemistry, colorimetry, or spectroscopy), are promising and suitable strategies for reliable disease marker analysis. − The response signals triggered by target synchronization are relatively independent and could be mutually cross-checked, which effectively reduces false positive/negative errors and improves the reliability of clinical diagnosis. , Since the initial exploration of PEC-involved dual-mode detection by Zhao, Chen and co-workers, many multimode sensing methods have been developed in biomolecule detection, including PEC-fluorescence, , PEC-electrochemistry, , PEC-colorimetry , and PEC-photothermal imaging. , Despite the superiority of these studies, the PEC involved multimode analysis, which remains at an early stage of development. Furthermore, due to the challenges and difficulties in the nanoengineering of multifunctional photoactive materials and the corresponding signal transduction routes, most multimode systems were limited to dual-mode assays, and systems involved individual components or different reaction processes, which may suffer from incompatible reactions and inevitable interference for each mode .…”