Colorimetric detection of aflatoxins B1 and M1 using aptamers and gold and silver nanoparticles

Ebanks, Fiona; Nasrallah, Hadi; Garant, Timothy M.; McConnell, Erin M.; DeRosa, Maria C.

doi:10.1016/j.aac.2023.07.003

Cited by 11 publications

(3 citation statements)

References 69 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thiolated DNA aptamers can be covalently conjugated to the surface of golden nanoparticles via gold-thiol bonds or by physisorption to the surface of the nanoparticles. For non-thiolated DNA aptamers, chemical interaction forces (such as hydrophobic effects and van der Waals forces) between the atoms of the DNA bases lead to strong adsorption on the surface of the nanoparticles [94].…”

Section: Optical Biosensorsmentioning

confidence: 99%

Biosensors for Food Mycotoxin Determination: A Comparative and Critical Review

Pisoschi,

Iordache,

Stanca

et al. 2024

Chemosensors

View full text Add to dashboard Cite

The need for performant analytical methodologies to assess mycotoxins is vital, given the negative health impact of these compounds. Biosensors are analytical devices that consist of a biological element for recognizing the analyte and a transducer, which translates the biorecognition event into a signal proportional to the analyte concentration. The biorecognition elements can be enzymes, antibodies, or DNA fragments. The modalities of detection can be optical, electrochemical, thermal, or mass-sensitive. These analytical tools represent viable alternatives to laborious, expensive traditional methods and are characterized by specificity given by the biorecognition element, sensitivity, fast response, portability, multi-modal detection, and the possibility of in situ application. The present paper focuses on a comprehensive view, enriched with a critical, comparative perspective on mycotoxin assay using biosensors. The use of different biorecognition elements and detection modes are discussed comparatively. Nanomaterials with optical and electrochemical features can be exploited in association with a variety of biorecognition elements. Analytical parameters are reviewed along with a broad range of applications.

show abstract

Section: Optical Biosensorsmentioning

confidence: 99%

Biosensors for Food Mycotoxin Determination: A Comparative and Critical Review

Pisoschi,

Iordache,

Stanca

et al. 2024

Chemosensors

View full text Add to dashboard Cite

show abstract

“…Nucleic acid-based synthetic receptors, Apts, have been successfully selected for mycotoxins with high binding affinity and selectivity, and have been incorporated into many sensor platforms. Based on the optical properties of metallic NPs, aptamer-NPs assays were developed in various formats including solution-and paper-based setups that enabled cheap, fast, and sensitive testing for the detection of mycotoxins in contaminated food [136].…”

Section: Aptamer-based Biosensorsmentioning

confidence: 99%

Current Trends in Mycotoxin Detection with Various Types of Biosensors

Majer-Baranyi,

Adányi,

Székács

2023

Toxins

View full text Add to dashboard Cite

One of the most important tasks in food safety is to properly manage the investigation of mycotoxin contamination in agricultural products and foods made from them, as well as to prevent its occurrence. Monitoring requires a wide range of analytical methods, from expensive analytical procedures with high-tech instrumentation to significantly cheaper biosensor developments or even single-use assays suitable for on-site monitoring. This review provides a summary of the development directions over approximately a decade and a half, grouped according to the biologically sensitive components used. We provide an overview of the use of antibodies, molecularly imprinted polymers, and aptamers, as well as the diversity of biosensors and their applications within the food industry. We also mention the possibility of determining multiple toxins side by side, which would significantly reduce the time required for the analyses.

show abstract

“…This aggregation phenomenon can also be followed by a naked-eye color change due to the electromagnetic coupling among the SPR of nearby AuNPs (6). In the literature, various studies reported the analysis of AFB 1 using chromogenic reactions based on noble metal nanoparticles (7)(8)(9)(10). However, colorimetric methodologies using the aggregation or anti-aggregation approach for the detection of AFB 1 are still meager.…”

Section: Introductionmentioning

confidence: 99%

Colorimetric detection of the potent carcinogen aflatoxin B1 based on the aggregation of L-lysine-functionalized gold nanoparticles in the presence of copper ions

Sánchez-Carrillo,

Quintanar-Guerrero,

José-Yacamán

et al. 2024

Front. Nutr.

View full text Add to dashboard Cite

L-lysine functionalized gold nanoparticles (AuNPs-Lys) have been widely used for the detection of worldwide interest analytes. In this work, a colorimetric assay for the detection of the carcinogen aflatoxin B1 (AFB1) based on the aggregation of AuNPs-Lys in the presence of copper ions was developed. For this purpose, AuNPs were synthesized in citrate aqueous solution, functionalized, and further characterized by UV–Vis, fluorescence, Fourier transform infrared spectroscopy (FTIR), nanoparticle tracking analysis (NTA), dynamic light scattering (DLS), and transmission electron microscopy (TEM). In general, AuNPS-Lys (~2.73 × 1011 particles) offered a clear colorimetric response in the presence of AFB1 and Cu2+ ions showing linearity in the range of 6.25 to 200 ng AFB1/mL, with a detection limit of 4.18 ng AFB1/mL via photometric inspection. Moreover, the performance of the proposed methodology was tested using the 991.31 AOAC official procedure based on monoclonal antibodies in maize samples artificially contaminated with AFB1. There was a good agreement between the measured AFB1 concentrations in both assays, the average recoveries for the colorimetric and immunoaffinity assays were between 91.2–98.4% and 96.0–99.2%, respectively. These results indicated that the colorimetric assay could be used as a rapid, eco-friendly, and cost-effective platform for the quantification of AFB1 in maize-based products.

show abstract

Colorimetric detection of aflatoxins B1 and M1 using aptamers and gold and silver nanoparticles

Cited by 11 publications

References 69 publications

Biosensors for Food Mycotoxin Determination: A Comparative and Critical Review

Biosensors for Food Mycotoxin Determination: A Comparative and Critical Review

Current Trends in Mycotoxin Detection with Various Types of Biosensors

Colorimetric detection of the potent carcinogen aflatoxin B1 based on the aggregation of L-lysine-functionalized gold nanoparticles in the presence of copper ions

Contact Info

Product

Resources

About