Colorimetric detection of Gadidae species using probe-modified gold nanoparticles

Feng, Jia; Zhu, Qinchao; Wang, Yi

doi:10.1016/j.foodcont.2020.107287

Cited by 10 publications

(5 citation statements)

References 38 publications

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“…In Table 1, we compared the performance of the proposed method with the previously reported colorimetric methods for detection of specific sequence using AuNPs in the literature since 2020 (21)(22)(23)(24)(25)(26)(27)(28)(29). Although the sensitivity (LOD) of this work is not comparable to other methods which require complicated experimental steps including labeling, immobilization, enzyme amplification but the excellent simplicity, ease of synthesis of AuNPs (in situ synthesis), free labeling (via poly guanine) and no need to special reagents are the advantages of this work.…”

Section: Analytical Performancementioning

confidence: 99%

Building Polyvalent DNA‐Functionalized Anisotropic AuNPs using Poly‐Guanine‐Mediated In‐Situ Synthesis for LSPR‐Based Assays: Case Study on OncomiR‐155

Borghei

Hosseinkhani

2022

Photochem & Photobiology

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DNA-functionalized gold nanoparticles (DNA-AuNPs) hold great promise for numerous biomedical applications, especially the building of well-defined nanosystems. Previously reported methods for the preparation of DNA-AuNPs all rely on the use of DNA-bearing free thiol or disulfide groups at their 3 0 /5 0 ends. But here we report a novel polyvalent DNA-AuNPs conjugation approach by in-situ fast synthesis of AuNPs at the polyguanine (G 12 ) strands. As confirmed by both TEM images and gel electrophoresis analysis, many poly G strand can form an individual anisotropic AuNP and so each AuNP functionalized with a dense layer of DNA, resulting in the formation of polyvalent (p)DNA-AuNPs. The general applicability of this novel approach was further verified in hybridization test and UV-Vis spectroscopy results show that pDNA-AuNPs conjugation is more attractive in biomedical diagnosis and specific sequence detection like microRNA-155 by using an extra-strand poly G with "sticky end" that are complementary to the target sequence.

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Section: Analytical Performancementioning

confidence: 99%

Building Polyvalent DNA‐Functionalized Anisotropic AuNPs using Poly‐Guanine‐Mediated In‐Situ Synthesis for LSPR‐Based Assays: Case Study on OncomiR‐155

Borghei

Hosseinkhani

2022

Photochem & Photobiology

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“…However, after incubation with 40 mM NaCl, the intensity of the absorbance peak at 524 nm dramatically decreased, implying that the AuNPs aggregated (curve 3 in Figure 2). This is due to the negative charges of citrates coated on the AuNP surface being neutralized by Na + ions, leading to a reduction in electrostatic repulsion and subsequent AuNP aggregation [9,24]. DNA aptamers have been reported to be adsorbed onto the AuNP surface, thus enhancing the electrostatic repulsion between nanoparticles, and protecting AuNPs from NaCl-induced aggregation [22,33].…”

Section: Feasibility Of the Colorimetric Assay For Atx-a Detectionmentioning

confidence: 99%

“…The ATX-a aptamer undergoes a conformational change induced by the specific binding of aptamers and ATX-a. This change reduces the aptamer adsorption on the AuNP surface, thus diminishing the protective effect of the aptamer and leading to AuNP aggregation [1,9].…”

Section: Feasibility Of the Colorimetric Assay For Atx-a Detectionmentioning

confidence: 99%

“…In recent years, aptamer-based colorimetric sensing methods have emerged as alternatives to detect various target molecules [8][9][10][11][12][13], because of their simplicity, low cost, high sensitivity, and specific detection without the requirement of complex instrumentation [14]. In this assay, aptamers (single-stranded DNA or RNA oligonucleotides) are used as recognition elements owing to their ability to selectively bind their target molecules, which helps improve the sensor specificity [15][16][17][18][19][20].…”

Section: Introductionmentioning

confidence: 99%

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A Simple and Ultrasensitive Colorimetric Biosensor for Anatoxin-a Based on Aptamer and Gold Nanoparticles

Nguyen

Jang

2021

Micromachines

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Here, we designed a simple, rapid, and ultrasensitive colorimetric aptasensor for detecting anatoxin-a (ATX-a). The sensor employs a DNA aptamer as the sensing element and gold nanoparticles (AuNPs) as probes. Adsorption of the aptamer onto the AuNP surface can protect AuNPs from aggregation in NaCl solution, thus maintaining their dispersion state. In the presence of ATX-a, the specific binding of the aptamer with ATX-a results in a conformational change in the aptamer, which facilitates AuNP aggregation and, consequently, a color change of AuNPs from red to blue in NaCl solution. This color variation is directly associated with ATX-a concentration and can be easily measured using a UV/Vis spectrophotometer. The absorbance variation is linearly proportional to ATX-a concentration across the concentration range of 10 pM to 200 nM, with a detection limit of 4.45 pM and high selectivity against other interferents. This strategy was successfully applied to the detection of ATX-a in lake water samples. Thus, the present aptasensor is a promising alternative method for the rapid detection of ATX-a in the environment.

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“…However, these methods require expensive equipment, are complicated to operate, require large samples, are time-consuming and costly, involve labor-intensive sample preparation, and require a large number of organic solvents that are toxic to humans [ 14 , 15 ]. The limitations of these methods have led to the development of other assays, such as colorimetric sensing, which have been developed and widely used to detect various target molecules due to their simplicity, affordability, high sensitivity, and high specificity [ 16 , 17 , 18 ]. Due to the localized surface plasmon resonance (LSPR) characteristics of noble metals such as gold and silver nanoparticles, the wavelength of light absorbed by metal NPs is affected, resulting in changes in color that can be used in the colorimetric detection of pollutants [ 19 , 20 ].…”

Section: Introductionmentioning

confidence: 99%

Colorimetric Detection of Acenaphthene and Naphthalene Using Functionalized Gold Nanoparticles

Chuang

Dong

Laishram

et al. 2023

IJMS

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Polycyclic aromatic hydrocarbons are a class of chemicals that occur naturally. They generally demonstrate a high degree of critical toxicity towards humans. Acenaphthene and naphthalene contain compounds that are commonly found in the environment as compared to other PAHs. Consequently, a reliable method of detecting PAHs is crucial for the monitoring of water quality. A colorimetric method based on sodium nitrite-functionalized gold nanoparticles was developed in this study for acenaphthene and naphthalene detection. Different functionalized parameters are determined for the optimization of assay conditions. A linear relationship was found in the analyte concentration range of 0.1–10 ppm with the limit of detection for acenaphthene and naphthalene being 0.046 ppm and 0.0015 ppm, respectively, under the optimized assay conditions. The method’s recovery rate for actual samples falls within the range of 98.4–103.0%. In selective and anti-interference tests, the presence of cations and anions has minimal impact on the detection of the analyte. The colorimetric detection method proposed in this study effectively determines the presence of the analyte in real water samples and has a high recovery rate.

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Colorimetric detection of Gadidae species using probe-modified gold nanoparticles

Cited by 10 publications

References 38 publications

Building Polyvalent DNA‐Functionalized Anisotropic AuNPs using Poly‐Guanine‐Mediated In‐Situ Synthesis for LSPR‐Based Assays: Case Study on OncomiR‐155

Building Polyvalent DNA‐Functionalized Anisotropic AuNPs using Poly‐Guanine‐Mediated In‐Situ Synthesis for LSPR‐Based Assays: Case Study on OncomiR‐155

A Simple and Ultrasensitive Colorimetric Biosensor for Anatoxin-a Based on Aptamer and Gold Nanoparticles

Colorimetric Detection of Acenaphthene and Naphthalene Using Functionalized Gold Nanoparticles

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