Colorimetric detection of microRNA based hybridization chain reaction for signal amplification and enzyme for visualization

Ying, Na; Sun, Tao; Chen, Zhibao; Song, Guangping; Qi, Bingyao; Bu, Shengjun; Sun, Xiuwei; Wan, Jiayu; Li, Zehong

doi:10.1016/j.ab.2017.04.007

Cited by 46 publications

(11 citation statements)

References 24 publications

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“…For example, Ying et al. (2017) proposed a colorimetric detection for miR‐155 detection . In their strategy, miRNA served as a linker to recognize the capture probe and trigger on the interface.…”

Section: Isothermal Nucleic Acid Signal Amplification Combined With Omentioning

confidence: 99%

Application of Isothermal Nucleic Acid Signal Amplification in the Detection of Hepatocellular Carcinoma‐Associated MicroRNA

et al. 2018

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Hepatocellular carcinoma (HCC) is a common type of malignant tumor with high incidence and mortality, and is the third leading cause of cancer‐related death in human. High‐precision detection of tumor markers, such as microRNA (miRNA), is expected to contribute to the early diagnosis of HCC as well as improve the survival rate of HCC patients. In recent years, great effort has been made to develop different methods to detect HCC‐associated miRNA, in which signal amplification strategies are incorporated to realize high sensitivity. Among them, isothermal nucleic acid signal amplification is an emerging signal amplification technology which has the advantages of high amplification efficiency, good reproducibility, and easy operation for real‐time analysis. Herein, we provide an overview of the advances in the application of isothermal nucleic acid signal amplification in HCC‐associated miRNA detection by highlighting several representative works in recent years.

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Section: Isothermal Nucleic Acid Signal Amplification Combined With Omentioning

confidence: 99%

Application of Isothermal Nucleic Acid Signal Amplification in the Detection of Hepatocellular Carcinoma‐Associated MicroRNA

et al. 2018

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“…However, these advantages are at the cost of tedious two-enzymatic reaction, expensive fluorophore and MGB modification, as well as high dependence on advanced real-time fluorescent quantitative PCR machine, which limit its application in routine tests and screening. Therefore, a large family of label-free, , colorimetric, − and electrochemical − miRNA detection methods have been developed, which are almost isothermal amplification methods, in order to address the similar problems in RT-PCR. Even though these methods can be performed without precise control of temperature cycling, label-free, or reporting signal easily, the nature that the specificity control most relies on simple one-check hybridization between target miRNA and their recognition element makes them less reliable for the detection of miRNAs as disease biomarkers.…”

mentioning

confidence: 99%

Colorimetric PCR-Based microRNA Detection Method Based on Small Organic Dye and Single Enzyme

et al. 2018

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microRNAs (miRNAs) have been a class of promising disease diagnostic biomarkers and therapeutic targets for their important biological functions. However, because of the high homology, interference from precursors (pri-miRNA, pre-miRNA), as well as limitations in the current assay technologies, it poses high demand and challenge for a specific, efficient, and economic miRNA assay method. Here, we propose a new miRNA detection method based on a label-free probe and a small organic dye with sequence dependence, realizing the sequence-specific and colorimetric detection of target miRNA. What is pleasantly surprising, only one enzyme is enough to propel the whole miRNA assay process, greatly simplifying the reaction component and detection process. Together with PCR amplification for the high enough sensitivity and three checks for specificity control, a detection limit of 5 fM was obtained and even one mutation could be discriminated visually. Overall, the new method makes much progress in convenience and economy of PCR-based miRNA assay method so that miRNA assay is going to be more friendly and affordable.

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“…At the same time, amplification is carried out under both homogeneous and heterogeneous conditions. [20][21][22][23] The results reported previously have indicated that both approaches permit the development of highly sensitive assays of miRNAs. However, no direct comparative study of these two formats has been carried out, which leaves unanswered the question of which of these formats of amplification, homogeneous or heterogeneous, is preferable to use.…”

Section: Introductionmentioning

confidence: 99%

Comparison of chemiluminescent heterogeneous and homogeneous–heterogeneous assays coupled with isothermal circular strand-displacement polymerization reaction amplification for the quantification of miRNA-141

Solovjev

Galkin

Медведько

et al. 2022

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Colorimetric detection of microRNA based hybridization chain reaction for signal amplification and enzyme for visualization

Cited by 46 publications

References 24 publications

Application of Isothermal Nucleic Acid Signal Amplification in the Detection of Hepatocellular Carcinoma‐Associated MicroRNA

Application of Isothermal Nucleic Acid Signal Amplification in the Detection of Hepatocellular Carcinoma‐Associated MicroRNA

Colorimetric PCR-Based microRNA Detection Method Based on Small Organic Dye and Single Enzyme

Comparison of chemiluminescent heterogeneous and homogeneous–heterogeneous assays coupled with isothermal circular strand-displacement polymerization reaction amplification for the quantification of miRNA-141

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