Colorimetric determination of 3,6-anhydrogalactose in polysaccharides from red seaweeds

Matsuhiro, Betty; Zanlungo, Alberto B.

doi:10.1016/0008-6215(83)88056-2

Cited by 37 publications

(12 citation statements)

References 5 publications

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“…Chemical analysis Total sugars were determined by the phenol-sulfuric acid method using D-galactose as standard (Chaplin 1986). The content of 3,6-anhydrogalactose was determined according to Matsuhiro and Zanlungo (1983) using D-fructose as standard (Matsuhiro et al 2014). The amount of sulfate was determined by a modification of Dodgson and Price method (Barahona et al 2012).…”

Section: Methodsmentioning

confidence: 99%

Carrageenans from nuclear phases of subantartic Mazzaella laminarioides (Gigartinales, Rhodophyta) and graft copolymerization of alkali-modified carrageenan with acrylamide

et al. 2015

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Aqueous extraction of cystocarpic, tetrasporic, and vegetative individuals of Mazzaella laminariodes collected in the Magellan Ecoregion, Southern Chile, afforded sulfated galactans. Analysis by Fourier transform infrared spectroscopy (FT-IR) and by 1 H and 13 C NMR spectroscopy indicated that purified extracts from cystocarpic (CP) and vegetative individuals (VP) were mixtures of κ/ι-type carrageenans and their precursors μ-and ν-carrageenans, whereas the aqueous extract from tetrasporic individuals was a λ-type carrageenan. Alkaline treatment of CP and VP polysaccharides produced modified polysaccharides with lower content of sulfate groups and with a concomitant increase of 3,6-anhydrogalactose content; analysis by 2D 1 H and 13 C NMR indicated the presence of hybrid κ-ι-carrageenans. Copolymerization of alkalitreated CP carrageenan with acrylamide was achieved by using ceric ammonium nitrate as the initiator; studies by NMR spectroscopy indicated grafting of polyacrylamide at position C-6 of β-galactopyranosyl residues. Reaction of modified CP with acrylamide assisted by microwave irradiation in the presence of ammonium persulfate afforded a copolymer grafted at position C-2 of β-galactopyranosyl residues.

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Section: Methodsmentioning

confidence: 99%

Carrageenans from nuclear phases of subantartic Mazzaella laminarioides (Gigartinales, Rhodophyta) and graft copolymerization of alkali-modified carrageenan with acrylamide

et al. 2015

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“…AG residues in galactans are quite unstable on acid hydrolysis and methanolysis, which are commonly used before the compositional and structural analyses of polysaccharides. Consequently, quantitative determination of AG in galactans has been carried out entirely by colorimetric methods (2)(3)(4), and the detailed structures of AG-containing galactans have not been fully elucidated yet.…”

Section: 6-anhydrogalactose (Ag)mentioning

confidence: 99%

A Gas Chromatographic Method for the Sugar Analysis of 3,6-Anhydrogalactose-Containing Algal Galactans

Hama

Nakagawa

Kurosawa

et al. 1998

Analytical Biochemistry

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“…An aliquot was reduced with NaBH, and acetylated with acetic anhydride in dry pyridine. The acetylated alditol mixture was analysed by gas-liquid chromatography with systems D and E. The percentage of 3,6-anhydrogalactose was determined by the previouslydescribed procedure (Matsuhiro & Zanlungo, 1983); methyl 3,6-anhydro-a-D-galactopyranoside synthesized by the method of Lewis et al (1963) was used as the standard sugar. The content of galactose and 6-0-methylgalactose was calculated by subtracting the anhydrogalactose content from the total sugar content determined by the anthrone method of Yaphe (1960).…”

Section: Methodsmentioning

confidence: 99%

Agars from Chilean Gelidiaceae

Matsuhiro

Urzúa

1991

Hydrobiologia

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Cystocarpic, tetrasporic and vegetative thalli of Gelidium chilense were extracted with water at 95 " C. The contents of 3,6-anhydro-galactose and sulfate group of the hydrocolloids correspond to those of an agar-type polysaccharide. The percentages of 6-O-methyl-galactose and of pyruvic acid ranged between 5.7-6.2% and 0.42-0.54%, respectively. The gel melting and gelation temperatures of Gelidium chilense, G. rex and G. lingulatum agars were determined. A correlation between 6-O-methylgalactose content and gelation temperatures was not observed. It was found by anion-exchange chromatography that 19.8% of tetrasporic and 4.9% of vegetative G. chilense agars are unsulfated polymers. Structural studies on the neutral fraction from tetrasporic G. chilense agar by partial hydrolysis and 'H NMR spectroscopy have shown that it is mainly composed of agarose. Methylation analysis, oxidative hydrolysis and partial hydrolysis, followed by 'H NMR spectroscopy, have shown that the neutral fraction of the agar from tetrasporic Gelidium rex is agarose. The results obtained in this work are compared with previously reported data on studies of agars from Chilean Gelidium species.

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Colorimetric determination of 3,6-anhydrogalactose in polysaccharides from red seaweeds

Cited by 37 publications

References 5 publications

Carrageenans from nuclear phases of subantartic Mazzaella laminarioides (Gigartinales, Rhodophyta) and graft copolymerization of alkali-modified carrageenan with acrylamide

Carrageenans from nuclear phases of subantartic Mazzaella laminarioides (Gigartinales, Rhodophyta) and graft copolymerization of alkali-modified carrageenan with acrylamide

A Gas Chromatographic Method for the Sugar Analysis of 3,6-Anhydrogalactose-Containing Algal Galactans

Agars from Chilean Gelidiaceae

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