“…They defined the volume of mobile solvent required for the elution of each component, giving the correction of these volumes for variations in temperature. The same authors (44) described the analysis of commercial estrogenic preparations, including oil solutions, tablets, and suspensions. The mixture of a-dihydroequilin and a-and 0-estradiol was separated from nonestrogenic impurities, traces of estrone and ecluilin, and the dihydroequilenins, and the individual components of the mixture were determined by a differential colorimetric procedure.…”