Colorimetric Determination of Noradrenaline and Adrenaline

Euler, U. S. v.; Hamberg, Ulla

doi:10.1111/j.1748-1716.1949.tb00636.x

Cited by 204 publications

(28 citation statements)

References 9 publications

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“…For this reason extraction with alcohol should be used for paper chromatographic experiments with extracts not subjected to further purification. A displacement of a similar kind has been noted previously after adsorption on alumina from trichloracetic acid extracts (Euler, 1950).…”

Section: Resultssupporting

confidence: 50%

Colorimetric and Biological Estimation of Adrenaline and Noradrenaline in Suprarenals of Guinea‐pigs

Euler

Hökfelt

1953

British Journal of Pharmacology and Chemotherapy

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Section: Resultssupporting

confidence: 50%

Colorimetric and Biological Estimation of Adrenaline and Noradrenaline in Suprarenals of Guinea‐pigs

Euler

Hökfelt

1953

British Journal of Pharmacology and Chemotherapy

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“…The perfusates (15 ml) were analysed for total catecholamines by the colourimetric method of von Euler & Hamberg (1949) using citrate-phosphate buffer at pH 6-0 (Mcllvaine, 1921). Samples of the perfusates collected for one min 60 to 90 min after beginning perfusion contained between 0-2 and 0-7 ,umol of catecholamines.…”

Section: Methodsmentioning

confidence: 99%

Pentobarbitone inhibition of catecholamine secretion

Holmes

Schneider

1973

British J Pharmacology

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Summary1. The perfused isolated cow adrenal gland was used to investigate the effect of barbituric acid, phenobarbitone and pentobarbitone on catecholamine secretion. 2. Pentobarbitone reduced catecholamine secretion induced by a number of drugs which cause exocytosis. The concentration of pentobarbitone which caused a 50% inhibition of catecholamine secretion was for acetylcholine 56x 1O-5M, for carbachol 6-3 X 10-5M, for histamine 16X 10-4M, for (+)-amphetamine 4 4 x 10-5M and for potassium chloride 1V5 x 10-4M. The degree of inhibition by pentobarbitone was not dependent on the concentration of the secretagogue. 3. Pentobarbitone (up to 10-3M) did not inhibit the catecholamine release that was induced by acetyldehyde or by calcium chloride; it inhibited slightly (34%) the catecholamine secretion induced by tyramine.4. Catecholamine release induced by carbachol was also inhibited by phenobarbitone (50% inhibition at 28 X 10-4M (n=7)) but was unaffected by barbituric acid. 5. Pentobarbitone had no effect on spontaneous or on (+)-amphetamineor tyramine-induced release of catecholamines from isolated chromaffin vesicles of cow adrenal medulla. 6. It is concluded that pentobarbitone inhibits catecholamine release by preventing a configurational change in the structure of the membrane of the chromaffin cell which is a necessary link between receptor activation and catecholamine release.

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“…or less, in a series of six separate injections, each separated by 30 sec. Chemical assays Perfusates from the glands and extracts of chromaffin granules (prepared according to Smith & Winkler, 1967a, b) were analysed for catecholamines by the colorimetric method of Euler & Hamberg (1949), using citrate-phosphate buffer (McIlvaine, 1921). In the calculation of the results, catecholamines were expressed in terms of adrenaline.…”

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confidence: 99%