Colorimetric Determination of Pectic Substances

McComb, E. A.; McCready, R. M.

doi:10.1021/ac60070a036

Cited by 316 publications

(87 citation statements)

References 7 publications

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“…Pectin concentration was determined by the colorimetric method (21). Michaelis constants (Ki) were calculated form Lineweaver-Burk double reciprocal (1/V versus I/S) plots.…”

Section: Kinetic Studiesmentioning

confidence: 99%

Purification and Characterization of Pectinmethylesterase from Ficus awkeotsang Makino Achenes

Tian

Liu²,

Chen³

et al. 1989

Plant Physiol.

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“…Pectin concentration was determined by the colorimetric method (21). Michaelis constants (Ki) were calculated form Lineweaver-Burk double reciprocal (1/V versus I/S) plots.…”

Section: Kinetic Studiesmentioning

confidence: 99%

Purification and Characterization of Pectinmethylesterase from Ficus awkeotsang Makino Achenes

Tian

Liu²,

Chen³

et al. 1989

Plant Physiol.

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“…N content of all samples was determined by microKjeldah1 and ammonia content of duodenal digesta by an alkaline phenate method (Gehrke et al 1968). Cellulose contents of feed, digesta and faecal samples were estimated according to the method of Crampton & Maynard (1938), neutral-and acid-detergent fibre contents of the feeds by the method of Van Soest & Wine (1967), and pectin contents by the method of McComb & McReady (1952).…”

Section: Analysesmentioning

confidence: 99%

The digestion of fresh perennial ryegrass (Lolium perenneL. cv. Melle) and white clover (Trifolium repensL. cv. Blanca) by growing cattle fed indoors

et al. 1985

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1. Pure swards ofperennial ryegrass (Loliumperenne L. cv. Melle) or white clover (Trifolium repens L.cv. Blanca) were harvested daily at three and two stages of growth respectively, and offered to housed cattle. The grass diets comprised primary growth (May) and two later regrowths of contrasting morphology (i.e. leaf stem values of 1.54 and 2 4 4 respectively), and were characterized by high contents of water-soluble carbohydrate and neutraldetergent fibre and comparable in vitro dry matter (DM) digestibilities (mean 0.80). Total nitrogen content was higher on primary growth grass (34 g/kg DM) than on regrowths (23 g/kg DM) but lower than values obtained for the two clover diets (38 and 43 g/kg DM, respectively). The clover diets had lower water-soluble carbohydrate contents than the grasses, comparable cellulose, but lower neutral-detergent fibre contents and in vitro DM digestibilities of 0.70 and 0.77 respectively.2. The experiment lasted from May until August, during which time a total of twenty-one young Friesian steers (initial average live weight 130 kg) were used to determine both nutrient supply to the small intestine (twelve animals) and apparent digestibility (nine animals). Each diet was offered at three levels of DM intake (i.e. 18, 22 and 26 g/kg live weight). A further six steers, all fed at the rate of 22 g DM/kg live weight, were used to determine the metabolizable energy contents of the five diets by means of open-circuit calorimetry.3. The three grass diets and the later-cut clover had, as intended, quite similar in vivo organic matter digestibilities, but that of the earlier-cut clover was lower, and this was associated with a large number of flower heads in this crop at the time of feeding. 4.On the clover diets, proportionately less of the ingested organic matter appeared to be digested in the rumen (0.40) compared with the grass diets (0.58) (P < 0.001). On the high-N primary grass and the clover diets, substantial rumen losses of N were detected (P < 0.01) compared with regrowth grasses. 5.The metabolizable energy content of the primary growth of grass was 12.2 MJ/kg DM, whilst the values for the other two grass diets were lower (1 1.6 MJ/kg DM), despite no marked decline in overall energy digestibility. Values for the two clover diets (mean 10.5 MJ/kg DM) were considerably lower than all values noted for the grasses.6. The amount of absorbed non-ammonia-N expressed per MJ metabolizable energy averaged 1.24 g/MJ on the grass diets, with no apparent seasonal effects, compared with 1.79 g/MJ on the two clover diets.7. Reasons for variations in nutrient supply and the consequence of considerable rumen losses of ingested N on the high-N diets are discussed, and the contribution that white clover can make to overall protein supply in grazing ruminants is established.Despite the fact that most of the total forage utilized by ruminants throughout the world is consumed in situ in the fresh form by grazing animals, remarkably few in vivo experiments have been undertaken to examine the nutritive v...

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“…By contrast with the behaviour when fractions from Sephadex G 75 were applied to Ecteola-cellulose, A F activity was not present in the initial eluate, nor was it present in the 0.1 M-NaCl eluates of high PG activity (Table I). The uronjde concentration (McComb & McCready, 1952) was decreased from 1700 pg./ml. in the culture filtrate to 2 pg.[ml.…”

Section: R E S U L T Smentioning

confidence: 82%

The Partial Purification and Properties of Endopolygalacturonase and -L-Arabinofuranosidase secreted by Sclerotinia fructigena

Fielding

Byrde

1969

Journal of General Microbiology

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An endopolygalacturonase (PG) and an a-L-arabinofuranosidase (AF) in culture filtrates of the fungus Sclerotinia fructigena were partially purified by ion-exchange chromatography on CM-Sephadex and Ecteola-cellulose columns, and characterized. Molecular weight estimations by gel-filtration and electrophoresis on cellulose acetate strips indicated that both enzymes existed in multiple forms. The PG showed unusual stability to extreme pH values, to classical inhibitors, and to proteolytic enzymes; in the crude filtrate a bimodal temperature/stability curve was obtained.

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Colorimetric Determination of Pectic Substances

Cited by 316 publications

References 7 publications

Purification and Characterization of Pectinmethylesterase from Ficus awkeotsang Makino Achenes

Purification and Characterization of Pectinmethylesterase from Ficus awkeotsang Makino Achenes

The digestion of fresh perennial ryegrass (Lolium perenneL. cv. Melle) and white clover (Trifolium repensL. cv. Blanca) by growing cattle fed indoors

The Partial Purification and Properties of Endopolygalacturonase and -L-Arabinofuranosidase secreted by Sclerotinia fructigena

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