Colorimetric Determination of Phenylephrine hydrochloride Drug by Diazotization Reaction

Abstract

By diazotization coupling reaction is one of the most frequently employed technique in pharmaceutical analysis. It involves measuring the amount of ultraviolet or visible radiation absorbed by a substance in solution as a method for estimation drug of Phenylephrine hydrochloride by reliant on azo-coupling reaction between (PHE)HCl and Clonazepam as a reagent. To reach to the aim of obtaining the colored product with a maximum absorbance 466nm. The linearity range for Phenylephrine hydrochloride was (0.5_9μg\ml) while the detections limit 0.006 and quantification limit 0.02μg\ml The Molar Absorptivity 2.5457 ×104L/mol.cm with Sandal’s sensitivity 0.01μg\cm2. Eventually these methods were successfully completed to determination of PHE.HCl in pure and commercial form.

Paper microfluidic device and spectrophotometry methods for colorimetric detection of phenylephrine hydrochloride in pure and pharmaceutical preparations

“…Linear range LOD Remarks Ref Spectrophotometry 2-24 µg mL -1 0.278 μg mL -1 Coupling reaction with diazotized sulfacetamide sodium [9] Spectrophotometry 5-100 μg mL −1 Oxidative coupling with the use of 4-aminoantipyrine [10] Spectrophotometry 10-200 μg 10 mL -1 Di azo-coupling using 2,4dinitroaniline [11] Spectrophotometry 0.5-9 µg mL -1 0.006 µg mL -1 Diazotization Reaction using clonazepam [12] Electrochemical sensor 5.0 × 10 -6 -7.5 × 10 -4 mol L -1…”

Development of Spectrophotometric Method to Assay Phenylephrine Hydrochloride in Pure and in its Pharmaceutical Forms Using Diazotized Sulphanilic Acid

“…Most of these procedures based on the formation of diazotized PPh and coupling with 2,4-dinitroaniline (Hasan et al, 2020), clonazepam in presence of NH 4 OH to form a yellow solution measured at 466 nm. (Alteemi and Kadim, 2020), 2-aminothiazole in alkaline medium and followed by dispersive liquid-liquid microextraction method (Mzban et al, 2020), sulfacetamide sodium in alkaline medium of NaOH and the absorbance was measured at 425nm (Wasan, 2019), metoclopramide in an alkaline medium to give an intense orange colored product (λmax at 470) (Al-Abachi and Abed, 2015), p-nitroaniline (Ibraheem, 2009) and 2-aminobenzothiazole (Othman and Abdul Fatah, 2009). Others based on the oxidative coupling reactions of PPh with N,N-dimethyl-p-phenylenediamine in the presence of sodium persulphate (Radhia et al, 2021), N,Ndimethyl-p-phenylenediamine with FeCl3 in alkaline media (Ahmed et al, 2020) and with 4-aminoantipyrine and potassium ferricyanide (Aljeboree and Alshirifi, 2018).…”

Development of Two Simple Spectrophotometric Methods to Assay Phenylephrine-HCl as Pure Form And in Its Drug Forms

“…Most researches are described utilizing spectrophotometric methods for estimating PPH in the bulk and in its pharmaceutical formulations using different reactions and various reagents. Some of these methods involve diazotization and coupling reaction (19)(20)(21)(22)(23)(24). Other methods base on the oxidative coupling reaction of PPH with 4-aminoantipyrine and potassium ferricyanide to yield dirty ping water soluble product (25), N,N-dimethyl-p-phenylenediamine in the presence of sodium persulfate in basic medium to give panic solution soluble product with maximum absorbance at 502 nm (26), and N,N-dimethyl-pphenylenediamine dihydrochloride with ferric chloride in basic media to form green-blue soluble dye product (27,28).…”