Colorimetric Determination of Procaine Hydrochloride in Pharmaceutical Preparations

Salama, R.B.; Omer, A.I.H.

doi:10.1002/jps.2600690326

Cited by 8 publications

(1 citation statement)

References 2 publications

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“…In the Chinese Pharmacopoeia, however, PRC is titrated with sodium hydroxide and determined by thin layer chromatography (Liu et al, 2012). Other analytical methods have also been used, including colorimetry (Lee & Livett, 1967;Salama & Omer, 1980), spectrophotometry (Liu et al, 2000), gas chromatography (Baniceru, Croitoru, & Popescu, 2004;Ohshima & Takayasu, 1999), high performance liquid chromatography (He, Peng, Tang, & Zhang, 2013;Liu, Peng, et al, 2017;Lu, 2012), and liquid chromatography tandem mass spectrometry (Babic et al, 2010;Dhananjeyan et al, 2007). However, these methods are time consuming and expensive because they require long pre-treatment procedures and sophisticated analytical instruments (Ge, Suryoprabowo, Zheng, & Kuang, 2017;Liu, Suryoprabowo, Zheng, Song, & Kuang, 2017;Wang, Zheng, et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Development of an immunochromatographic test strip for the detection of procaine in milk

Hao

Suryoprabowo

Song

et al. 2018

Food and Agricultural Immunology

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Procaine (PRC) is a local anaesthetic used in the treatment of several disease related to ageing, asthma, and arthritis. In this study, we describe a rapid, sensitive, simple, and accurate method for the determination of PRC in milk, using an immunochromatographic test strip. For the immunochromatographic test, antigen (PRCbovine serum albumin) and goat anti-mouse IgG were drawn onto a nitrocellulose membrane representing the control line and test line. The test was developed with a sensitive monoclonal antibody specific for PRC, generated by immunizing BALB/c mice with a well-characterized PRC-Keyhole limpet hemocyanin conjugate, produced in our laboratory. Under optimized conditions, the semiquantitative test strip was able to detect PRC at concentrations down to 1 ng/mL in both 0.01 M phosphate buffer saline (pH 7.4) and milk samples. All results were obtained within 5 min. Our data indicate that the immunochromatographic assay is capable of sensitive, rapid, and specific on-site screening of PRC.

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