Colorimetric Determination of Some N-1-Substituted Nitroimidazoles

Lau, Edward; Yao, Chuanjin; Lewis, Margaret; Senkowski, Bernard Z.

doi:10.1002/jps.2600580109

Cited by 26 publications

(10 citation statements)

References 2 publications

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Section: Introductionmentioning

confidence: 99%

“…The hydrolysis reaction requires a strong alkaline medium (NaOH 0.1 to 4.0 mol L -1 ) and it can be completed in 15 min to 2 h if carried out in a boiling water bath. [12][13][14] At room temperature the hydrolysis requires several hours to be complete.…”

Section: The Hydrolysis Reactionmentioning

confidence: 99%

“…This approach has been taken advantage of to implement MTZ analytical procedures. [12][13][14] An assay based on measurement of the change in ultraviolet absorbance during the alkaline hydrolysis of the compound was proposed for determination of MTZ in oral suspensions. 12 Another one, based on colorimetric monitoring of the diazonium salt produced by a diazotizationcoupling reaction with the released nitrite ions, has long been proposed and optimized later on.…”

Section: Introductionmentioning

confidence: 99%

“…12 Another one, based on colorimetric monitoring of the diazonium salt produced by a diazotizationcoupling reaction with the released nitrite ions, has long been proposed and optimized later on. 13,14 Despite the good selectivity and sensitivity of these procedures, they are time consuming and employ large amounts of reagents.This work describes a flow injection analysis (FIA) approach for determination of MTZ based on the spectrometric monitoring of nitrite ions released from an alkaline hydrolysis pre-treatment of the sample. The method combines the high specificity of the diazotizationcoupling reaction for nitrite ions with advantages inherent to the FIA approaches.…”

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confidence: 99%

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Flow injection determination of metronidazole through spectrophotometric measurement of the nitrite ion produced upon alkaline hydrolysis

Simões¹,

Medeiros²,

Gaião³

et al. 2006

J. Braz. Chem. Soc.

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Um novo método para determinação de metronidazol, baseado no monitoramento espectrométrico do sal de diazônio produzido in-line pelos íons nitrito, liberados numa hidrólise alcalina, foi desenvolvido e aplicado com sucesso a comprimidos (r = 0,9993, 2,0-20,0 mg L -1 , LD = 0,7 mg L -1 ), sem interferência de ingredientes comumente presentes.A new method for metronidazole determination, based on spectrometric monitoring of a diazonium salt produced in-line by alkaline hydrolysis released nitrite ions, was developed and successfully applied to pharmaceutical tablets (r = 0.9993, 2.0-20.0 mg L -1 , DL = 0.7 mg L -1 ) with no interference from common ingredients accompanying the drug. Keywords: flow injection analysis, spectrophotometry, pharmaceuticals, metronidazole, nitrite IntroductionMetronidazole (MTZ), 2-(5-nitro-2-methylimidazol-1-yl)-ethanol, likewise others nitroimidazole derivatives presents biological activity against anaerobic micro-organisms, being largely used as the active ingredient of antihelminthic medicines. 1 Thus, there is an important demand for rapid and simple methods for the determination of MTZ in pharmaceutical preparations. Methods based on HPLC and classical volumetric titration are recommended by the pharmacopoeia for the direct determination of MTZ. 2 Although this later one is kind of simple, being daily practiced in many routine analysis laboratories, it is time consuming and very tedious as it is based on the use of acetic anhydride. Various research studies have been devoted to the development of new high performance alternative methods for determination of the drug in dosage forms using different techniques such as high performance liquid chromatography, 3 supercritical fluid chromatography, 4 differential pulse polarography 5 and voltammetry with glassy carbon electrode 6 or chemically modified electrodes. 7 Flow analysis approaches have also being successfully applied on developing fast analytical methods for determination of some antihelmintic and antiprotozoal with spectrometric 8-10 and biamperometric detection in dosage forms. 11 The alkaline hydrolysis of MTZ releases the nitro group as nitrite ion and the reaction can be carried out in a quantitative way by controlling the pH, temperature and hydrolysis time. Thus, a simple measurement of the yielded nitrite ions can be used to determine MTZ. This approach has been taken advantage of to implement MTZ analytical procedures. [12][13][14] An assay based on measurement of the change in ultraviolet absorbance during the alkaline hydrolysis of the compound was proposed for determination of MTZ in oral suspensions. 12 Another one, based on colorimetric monitoring of the diazonium salt produced by a diazotizationcoupling reaction with the released nitrite ions, has long been proposed and optimized later on. 13,14 Despite the good selectivity and sensitivity of these procedures, they are time consuming and employ large amounts of reagents.This work describes a flow injection analysis (FIA) approach for determination of MTZ based o...

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Section: Introductionmentioning

confidence: 99%

Section: The Hydrolysis Reactionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Flow injection determination of metronidazole through spectrophotometric measurement of the nitrite ion produced upon alkaline hydrolysis

Simões¹,

Medeiros²,

Gaião³

et al. 2006

J. Braz. Chem. Soc.

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“…A rapid and sensitive absorptiometric assay was reported by Lau et al (12). It is based on the alkaline hydrolysis of N-1-substituted 5-nitroimidazoles to yield stoichiometric amounts of the nitrite (-NO*) ion which after acidification is used to diazotize sulfanilamide and couple it to N-( 1 -naphthyl)ethylenediamine to produce the characteristic Bratton-Marshall azo dye chromophore (5).…”

mentioning

confidence: 99%

Absorptiometric, Polarographic, and Gas Chromatographic Assays for the Determination of N-1-substituted Nitroimidazoles in Blood and Urine

Silva

Munno

Strojny

1970

Journal of Pharmaceutical Sciences

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Direct Assay of Tinidazole in Human Serum by Micellar Liquid Chromatography

Habel

Guermouche

1997

Biomed. Chromatogr.

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A liquid chromatographic procedure for the direct determination of tinidazole in human serum is presented. It includes the use of a micellar mobile phase consisting of SDS (5.10−2 M): propan‐1‐ol; (94:6; v/v) and a μBondapak CN column with UV detection at 320 nm. No solvent extraction or deproteinization are necessary. The linearity (0.1–10 mg L), the precision (3%), the reproducibility (1.3%), the recovery (99%), and the detection limit (0.1 mg L) in the tinidazole determination are comparable and sometimes greater than the corresponding tinidazole parameters when deproteinization and conventional reversed‐phase HPLC are used. One hundred injections of serum samples do not affect the column life. The procedure is applied to ascertain the pharmacokinetics of 10 mg/kg of tinidazole. © 1997 by John Wiley & Sons, Ltd.

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Colorimetric Determination of Some N-1-Substituted Nitroimidazoles

Cited by 26 publications

References 2 publications

Flow injection determination of metronidazole through spectrophotometric measurement of the nitrite ion produced upon alkaline hydrolysis

Flow injection determination of metronidazole through spectrophotometric measurement of the nitrite ion produced upon alkaline hydrolysis

Absorptiometric, Polarographic, and Gas Chromatographic Assays for the Determination of N-1-substituted Nitroimidazoles in Blood and Urine

Direct Assay of Tinidazole in Human Serum by Micellar Liquid Chromatography

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