Colorimetric Estimation of Aluminum with Pyrocatechol Violet

Anton, Anthony

doi:10.1021/ac60162a052

Cited by 31 publications

(14 citation statements)

References 5 publications

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“…The ability to chelate the copper ion from the extracts was determined by the method described by Anton [ 48 ]. Pyrocatechol violet, the reagent used in this assay, has the ability to associate with certain cations, such as aluminum, copper, bismuth and thorium.…”

Section: Methodsmentioning

confidence: 99%

Does the Use of Chitosan Contribute to Oxalate Kidney Stone Formation?

et al. 2014

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Chitosan is widely used in the biomedical field due its chemical and pharmacological properties. However, intake of chitosan results in renal tissue accumulation of chitosan and promotes an increase in calcium excretion. On the other hand, the effect of chitosan on the formation of calcium oxalate crystals (CaOx) has not been described. In this work, we evaluated the antioxidant capacity of chitosan and its interference in the formation of CaOx crystals in vitro. Here, the chitosan obtained commercially had its identity confirmed by nuclear magnetic resonance and infrared spectroscopy. In several tests, this chitosan showed low or no antioxidant activity. However, it also showed excellent copper-chelating activity. In vitro, chitosan acted as an inducer mainly of monohydrate CaOx crystal formation, which is more prevalent in patients with urolithiasis. We also observed that chitosan modifies the morphology and size of these crystals, as well as changes the surface charge of the crystals, making them even more positive, which can facilitate the interaction of these crystals with renal cells. Chitosan greatly influences the formation of crystals in vitro, and in vivo analyses should be conducted to assess the risk of using chitosan.

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Section: Methodsmentioning

confidence: 99%

Does the Use of Chitosan Contribute to Oxalate Kidney Stone Formation?

et al. 2014

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“…The cupric chelating was evaluated as described previously [ 26 ]. Different concentrations of AsRE (0.1, 0.3, 0.5, 1.0, 1.5, and 2.0 mg/mL) were added to reagent solution (pyracotechol (4.0 mM) and cupric sulfate II pentahydrate (0.2 mM)) and measured at 632 nm.…”

Section: Methodsmentioning

confidence: 99%

In Vitro and In Vivo Antioxidant Activity of Agave sisalana Agro-Industrial Residue

et al. 2020

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Agave sisalana agro-industrial residue has considerable potential against damage associated with oxidative stress and skin aging. This study aims to demonstrate, in vitro and in vivo, the potential of Agave sisalana agro-industrial residue as a safe and effective alternative for the prevention of damage caused by oxidative stress and aging. The antioxidant activity was evaluated in vitro (total antioxidant capacity, reducing power, DPPH radical scavenging, metal chelating (Fe2+ and Cu2+), and hydroxyl radical scavenging) and in vivo using the Caenorhabditis elegans organism model. The extract showed in vitro antioxidant activity in all tests performed. Tests with C. elegans showed that the extract was able to reduce the intracellular levels of reactive oxygen species (ROS) and increase the survival rate of worms. A downregulation of gst-4::GFP expression suggests a direct action against free radicals. Agave sisalana agro-industrial residue extract (AsRE) can therefore be considered as a source of antioxidant biomolecules, and the use of this agro-industrial residue in a new production process can lead to sustainability and socioeconomic development.

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“…The mixture was shaken and incubated for 10 min at room temperature, and the absorbance of the mixture was measured at 562 nm against a blank. EDTA was used as the positive control (15 –17). …”

Section: Methodsmentioning

confidence: 99%

Phenolic profile and antioxidant activity from peels and seeds of melon (Cucumis melo L. var. reticulatus) and their antiproliferative effect in cancer cells

Rolim

Fidelis

Padilha

et al. 2018

Braz J Med Biol Res

100

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Melon (Cucumis melo L.) has high economic value and in recent years, its production has increased; however, part of the fruit is wasted. Usually, inedible parts such as peel and seeds are discarded during processing and consumption. Extracts of melon residues were prepared and their phenolic compounds, antioxidants and antiproliferative activities were evaluated. Total phenolic compounds were found in hydroethanolic, hydromethanolic, and aqueous extracts, especially for melon peel (1.016 mg gallic acid equivalent/100 g). Flavonoids total content found for melon peel aqueous extract was 262 µg of catechin equivalent (CA)/100 g. In all extracts of melon peel significant amounts of gallic acid, catechin, and eugenol were found. For total antioxidant capacity, reported as ascorbic acid equivalent, the hydroethanolic and hydromethanolic extracts in peels and hydromethanolic in seeds were 89, 74, and 83 mg/g, respectively. Different extracts of melon showed iron and copper ions chelating activity at different concentrations, especially melon peel aqueous extract, reaching values of 61% for iron and 84% for copper. The hydroethanolic extract of melon peel presented a significant ability for hydroxyl radicals scavenging (68%). To assess the antiproliferative potential in human cancer cell lines, such as kidney carcinoma, colorectal carcinoma, cervical adenocarcinoma and cervical carcinoma, MTT assay was performed. The proliferation was inhibited by 20–85% at extracts concentrations of 0.1–1.0 mg/mL in all cancer cell lines. The results suggest that melon residues extracts display a high antioxidant activity in in vitro assays and have effective biological activity against the growth of human tumor cells.

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Colorimetric Estimation of Aluminum with Pyrocatechol Violet

Abstract: Platinum boat with sampleF. Solid carbon dioxide C. Silver gauze G. Aluminum foil D.

Cited by 31 publications

References 5 publications

Does the Use of Chitosan Contribute to Oxalate Kidney Stone Formation?

Does the Use of Chitosan Contribute to Oxalate Kidney Stone Formation?

In Vitro and In Vivo Antioxidant Activity of Agave sisalana Agro-Industrial Residue

Phenolic profile and antioxidant activity from peels and seeds of melon (Cucumis melo L. var. reticulatus) and their antiproliferative effect in cancer cells

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