Colorimetric Estimation of Indoleacetic Acid

Gordon, Solon A.; Weber, Robert P.

doi:10.1104/pp.26.1.192

Cited by 2,224 publications

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“…The ability of bacteria to produce auxin was measured using the Salkowsky reaction [15]. Voges-Proskauer reaction was used to determine acetoin production [16].…”

Section: Isolation Of the Bacterial Strains From Soilmentioning

confidence: 99%

Survey of Plant Drought-Resistance Promoting Bacteria from Populus euphratica Tree Living in Arid Area

Wang

Ouyang

et al. 2014

Indian J Microbiol

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Two hundred and thirty-two bacterial strains were isolated from the rhizospheric soil of Populus euphratica which is the dominant tree living in extreme arid regions in northwest China. Some strains with plant growth-promoting bacteria related metabolic characteristics were able to promote drought resistance in plants after inoculation. Ten strains with the greatest effects increased the dry weight of wheat shoots from 0.5 to 34.4 %, and the surface area of the root systems from 12.56 to 212.17 % compared to the control after drought treatment whereas no obvious promoting effect was observed in normal water conditions. These 10 strains were identified to be of the genera Pseudomonas, Bacillus, Stenotrophomonas and Serratia by 16S rRNA (rrs) gene sequence alignment. Among these strains, Serratia sp. 1-9 and Pseudomonas sp. 5-23 were the two most effective strains. Both of them produced auxin and the production increased significantly when cultured under simulated drought conditions which are inferred to be the most plausible mechanism for their plant growth-promoting effect under drought stress.

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“…The ability of bacteria to produce auxin was measured using the Salkowsky reaction [15]. Voges-Proskauer reaction was used to determine acetoin production [16].…”

Section: Isolation Of the Bacterial Strains From Soilmentioning

confidence: 99%

Survey of Plant Drought-Resistance Promoting Bacteria from Populus euphratica Tree Living in Arid Area

Wang

Ouyang

et al. 2014

Indian J Microbiol

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“…After incubation, cell suspension (1 mL) was transferred into a tube and mixed vigorously with 2 mL of Salkowski's reagent (150 mL of concentrated H 2 SO 4 , 250 mL of distilled H 2 O, 7.5 mL of 0.5 M FeCl 3 Á6H 2 O) (Gordon and Weber 1951) and allowed to stand at room temperature for 20 min. Development of pink colour in the cell suspensions indicated positive reaction for indole acetic acid production.…”

Section: Indole Acetic Acid Productionmentioning

confidence: 99%

Rhizosphere colonization and arsenic translocation in sunflower (Helianthus annuus L.) by arsenate reducing Alcaligenes sp. strain Dhal-L

Cavalca

Corsini

Bachate

et al. 2013

World J Microbiol Biotechnol

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In the present study, six arsenic-resistant strains previously isolated were tested for their plant growth promoting characteristics and heavy metal resistance, in order to choose one model strain as an inoculum for sunflower plants in pot experiments. The aim was to investigate the effect of arsenic-resistant strain on sunflower growth and on arsenic uptake from arsenic contaminated soil. Based on plant growth promoting characteristics and heavy metal resistance, Alcaligenes sp. strain Dhal-L was chosen as an inoculum. Beside the ability to reduce arsenate to arsenite via an Ars operon, the strain exhibited 1-amino-cyclopropane-1-carboxylic acid deaminase activity and it was also able to produce siderophore and indole acetic acid. Pot experiments were conducted with an agricultural soil contaminated with arsenic (214 mg kg -1 ). A real time PCR method was set up based on the quantification of ACR3(2) type of arsenite efflux pump carried by Alcaligenes sp. strain Dhal-L, in order to monitor presence and colonisation of the strain in the bulk and rhizospheric soil. As a result of strain inoculation, arsenic uptake by plants was increased by 53 %, whereas ACR3(2) gene copy number in rhizospheric soil was 100 times higher in inoculated than in control pots, indicating the colonisation of strain. The results indicated that the presence of arsenate reducing strains in the rhizosphere of sunflower influences arsenic mobilization and promotes arsenic uptake by plant.

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“…IAA was detected colorimetrically in the supernatants of the bacterial cultures using Salkowski's reagent [20]. All strains were grown in OAB minimal medium, containing tryptophan, as previously described [25].…”

Section: Iaa Assaymentioning

confidence: 99%

Transformation of Azospirillum brasilense Cd with an ACC Deaminase Gene from Enterobacter cloacae UW4 Fused to the Tet r Gene Promoter Improves Its Fitness and Plant Growth Promoting Ability

Holguín¹,

Glick

2003

Microbial Ecology

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A B S T R A C TIt has been reported that PGPB, containing ACC deaminase, can cleave the plant ethylene precursor ACC and thereby lower ethylene concentration in a developing or stressed plant, protecting it against the deleterious effects of stress ethylene and facilitating the formation of longer roots. In a previous work we have demonstrated expression of the ACC deaminase gene (acdS) from Enterobacter cloacae UW4 under the control of the lac promoter in Azospirillum brasilense Cd. With the inference that a construct including the ACC deaminase gene under the control of a constitutive promoter weaker than the lac promoter might impose less metabolic load on Azospirillum and improve its fitness, it was decided to clone acdS under the control of a tetracycline resistance gene promoter. The ACC deaminase structural gene was fused to the Tet r gene promoter by overlap extension using PCR, cloned in pRK415, and transferred into A.brasilense Cd. The resulting transformants showed lower ACC deaminase activity than those with the lac promoter controlled acdS gene. However, acdS under the control of the Tet r gene promoter imposed lesser metabolic load on Azospirillum brasilense Cd. The result was significantly increased IAA synthesis and greater bacterial growth rate, as well as increased ability to survive on the surface of tomato leaves and to promote the growth of tomato seedlings.

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Colorimetric Estimation of Indoleacetic Acid

Cited by 2,224 publications

References 0 publications

Survey of Plant Drought-Resistance Promoting Bacteria from Populus euphratica Tree Living in Arid Area

Survey of Plant Drought-Resistance Promoting Bacteria from Populus euphratica Tree Living in Arid Area

Rhizosphere colonization and arsenic translocation in sunflower (Helianthus annuus L.) by arsenate reducing Alcaligenes sp. strain Dhal-L

Transformation of Azospirillum brasilense Cd with an ACC Deaminase Gene from Enterobacter cloacae UW4 Fused to the Tet r Gene Promoter Improves Its Fitness and Plant Growth Promoting Ability

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