1989
DOI: 10.1007/bf00510085
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Colorimetric growth assay for epidermal cell cultures by their crystal violet binding capacity

Abstract: The application of a simple, rapid, and inexpensive colorimetric growth assay was tested for human epidermal cells subcultured in uncoated plastic dishes. Cell layers were incubated with a crystal violet (CV) solution (0.2% with ethanol 2% in 0.5 M Tris-Cl buffer, pH 7.8) for 10 min at room temperature. After rinsing with 0.5 M Tris-Cl (pH 7.8) the cell layer was dried and decolorized with a sodium-dodecylsulfate solution (0.5% with ethanol 50% in 0.5 M Tris-Cl, pH 7.8) for 60 min at 37 degrees C. The extincti… Show more

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Cited by 69 publications
(45 citation statements)
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“…Lentivirus-transduced 22Rv1 cells were electroporated with small interference RNA (siRNA) targeted to AR exon 1 as described (6,17) and seeded at equal density on 24-well plates. At the indicated time points, cells were fixed and stained with crystal violet as described (21).…”
Section: Methodsmentioning
confidence: 99%
“…Lentivirus-transduced 22Rv1 cells were electroporated with small interference RNA (siRNA) targeted to AR exon 1 as described (6,17) and seeded at equal density on 24-well plates. At the indicated time points, cells were fixed and stained with crystal violet as described (21).…”
Section: Methodsmentioning
confidence: 99%
“…Cell number was determined by a colorimetric assay after crystal violet staining, basically as described (Bonnekoh et al, 1989). The growth curve was normalized by the measurement at t=0.…”
Section: Methodsmentioning
confidence: 99%
“…A standard procedure was used as previously described (18,19). Cells were plated into a 96-well plate (2,500 cells per well).…”
Section: In Vitro Cell Growth Assaymentioning
confidence: 99%