Colorimetric Microdetermination of Bromhexine Drug in Aqueous Solution

Abstract

A simple, accurate and sensitive method for determination of bromhexine hydrochloride drug in aqueous solution has been described. The procedure has been based on the reaction of this drug with an excess amount of p-dimethylaminobenzaldehyde (DAB) in acidic medium (pH = 1.6) and the presence of sodium dodecyl sulfate (SDS). The produced yellow color from this reaction has been followed spectrophotometrically at an absorbance maximum of 430 nm. Microgram amounts of bromhexine hydrochloride can be estimated with an accuracy of better than ±1.5% and a relative standard deviation of less than 3.5%. The method has been used for the determination of 0.41-82.5 mg.mL -1 with a molar extinction coefficient of 4699.1-3602.3 L.mol -1 .cm -1 . An application of the developed procedure to bulk bromhexine hydrochloride and some of its pharmaceutical preparations has been carried out. The effect of the presence of some other surfactants and common pharmaceutical additives has been investigated. A comparison of the presented method with that in the absence of SDS and the standard method of the British Pharmacopoeia has been explored. The suggestion, according to the results, is to use the developed method as standard in pharmaceutical applications.

Application of a Schiff base derived from sulfanilamide as an acid-base indicator

“…More *Corresponding author. E-mail: rakhalil64@yahoo.com recently, we have extended our investigations of the above reaction through using bromhexine drug [2,3] and three ȕ-lactams antibiotics [4] instead of sulfonamide. The colored solutions of these molecules do not show the above observed phenomenon of sulfanilamide.…”

Spectrophotometric Determination of Bromhexine Hydrochloride by Azo-Dye Formation Reaction

“…Several spectrophotometric methods have been reported for the estimation of bromhexine-HCl using different reagents such as, rose Bengal in sulphuric acid medium [3], citric acid-AC 2 O in chloroform medium [4], p-dimethylamino benzaldehyde in highly acidic medium [5], bromocresol purple [6], tropaeoline, naphthalene blue 12Br and azocarmon Greact acidic dyes in non aqueous medium (chloroform) [7], p-dimethylaminobenzaldehyde in the presence of sodium dodecyl sulphate [8], after diazotisation, the corresponding bromhexin diazonium salt is coupled with a suitable agent such as, N-(1-naphthyl) ethylenediamine [9], 1-naphtylamine [10], resorcinol [11], 2-naphtho [12], orcinol [13]. Also, derivative and mults wavelength [14] and first derivative spectrophotometric methods [15] have been used in determination bromhexine-HCl.…”

An electrochemical sensor based on poly(procaterol hydrochloride)/carboxyl multi-walled carbon nanotube for the determination of bromhexine hydrochloride

“…Thus the quantitative analysis of BrH is very important in some elds such as clinical chemistry and pharmaceutical production. Various analytical protocols have been developed to determine the BrH content in biological samples, namely UV-visible spectrophotometry, [2][3][4] high performance liquid chromatography (HPLC) with a UV detector, 5,6 the thin layer chromatography (TLC) densitometric method, 7 capillary isotachophoresis (ITP) with a conductimetric detector, 8 chemiluminescence 9 and electrochemical methods. [10][11][12] Compared with other methods, the electrochemical technique possesses the merits such as simple apparatus, ease of operation, excellent selectivity and high sensitivity.…”