Colorimetric paper-based sarcosine assay with improved sensitivity

Masumoto, Mayu; Ohta, Sera; Nakagawa, Misaki; Hiruta, Yuki; Citterio, Daniel

doi:10.1007/s00216-021-03682-0

Cited by 21 publications

(11 citation statements)

References 46 publications

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“…The result was similar to the detection of color intensity of AuNPs in the test zone of our μTAD using a smartphone application (Figure S15). Chitosan-modified cotton thread could enhance the wet strength and flow rate of the cotton thread to immobilize antibodies and improve a uniform scaffold by accumulating reagents. ,, So, biomolecules bound to AuNPs-Ab 2 have a higher possibility to conjugate with anti-Ab 1 and to accumulate in the test zone of μTAD in the presence of chitosan modification, which also increases the photothermal effect of the AuNPs. To reduce overall reagent consumption, we selected chitosan at 20.0 mg/mL for our method.…”

Section: Resultsmentioning

confidence: 99%

“…We characterized and studied the chitosan-modified cotton thread using a SEM image as described in Figure S6 and also its fluidic transport behavior in Figure S7 . 28,32,36,37 Afterward, the cotton thread was fixed onto the fabricated base device using scotch tape. As well, small absorption pads were adhered onto both ends of the cotton thread.…”

Section: Synthesis Of Aunps and Conjugation Of Antibodiesmentioning

confidence: 99%

“…This relates to signal enhancements resulting from the accumulation of more analytes and reagents in its matrix. This can be explained from the interaction of positively charged chitosan getting adsorbed on the negatively charged cellulose substrate and the possibility of covalent immobilization of biorecognition elements on the surface. , For instance, Li et al demonstrated fabrication of an environmentally stable hydrophilic μTAD after functionalizing with chitosan. Their method could dramatically increase the hydrophilicity and wicking rate of the thread, thereby reducing the analysis time and improving signal detection.…”

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confidence: 99%

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Highly Sensitive Photothermal Microfluidic Thread-Based Duplex Immunosensor for Point-of-Care Monitoring

Khachornsakkul,

Del-Rio-Ruiz,

Zeng

et al. 2023

Anal. Chem.

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Herein, we successfully developed a thread-based analytical device (μTAD) for simultaneous immunosensing of two biomolecules with attomolar sensitivity by using a photothermal effect. A photothermal effect exploits a strong light-to-heat energy conversion of plasmonic metallic nanoparticles at localized surface plasmon resonance. The key innovation is to utilize the cotton thread to realize this sensor and the use of chitosan modification for enhancing the microfluidic properties, for improving the efficiency of photothermal conversion, and for sensor stability. The developed μTAD sensor consists of (i) a sample zone, (ii) a conjugation zone coated with gold nanoparticles bound with an antibody (AuNPs-Ab 2 ), and (iii) a test zone immobilized with a capture antibody (anti-Ab 1 ). The prepared μTAD is assembled in a custom three-dimensional (3D) printed device which holds the laser for illumination and the thermometer for readout. The 3D-printed supportive device enhances signal response by focusing light and localizing the heat generated. For proof of concept, simultaneous sensing of two key stress and inflammation biomarkers, namely, cortisol and interleukin-6 (IL-6), are monitored using this technique. Under optimization, this device exhibited a detection linear range of 2.0−14.0 ag/mL (R 2 = 0.9988) and 30.0−360.0 fg/mL (R 2 = 0.9942) with a detection limit (LOD) of 1.40 ag/mL (∼3.86 amol/L) and 20.0 fg/mL (∼950.0 amol/L) for cortisol and IL-6, respectively. Furthermore, the analysis of both biomolecules in human samples indicated recoveries in the range of 98.8%−102.88% with the highest relative standard deviation being 3.49%, offering great accuracy and precision. These results are the highest reported sensitivity for these analytes using an immunoassay method. Our PT-μTAD strategy is therefore a promising approach for detecting biomolecules in resource-limited point-of-care settings.

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Section: Resultsmentioning

confidence: 99%

Section: Synthesis Of Aunps and Conjugation Of Antibodiesmentioning

confidence: 99%

mentioning

confidence: 99%

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Highly Sensitive Photothermal Microfluidic Thread-Based Duplex Immunosensor for Point-of-Care Monitoring

Khachornsakkul,

Del-Rio-Ruiz,

Zeng

et al. 2023

Anal. Chem.

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“…[ 1 ] More recently, filter paper has emerged as a popular substrate for developing analytical devices aimed at detecting analytes at the point of need, including disease biomarkers, pathogens, environmental pollutants, and explosives, among others. [ 2–12 ] Paper is indeed an advantageous material for sensor fabrication since it is widely available and inexpensive, making it ideal for rapid prototyping. [ 13,14 ] It is obtained from renewable sources and can be destroyed by incineration, which is important for developing single‐use devices.…”

Section: Introductionmentioning

confidence: 99%

“…[1] More recently, filter paper has emerged as a popular substrate for developing analytical devices aimed at detecting analytes at the point of need, including disease biomarkers, pathogens, environmental pollutants, and explosives, among others. [2][3][4][5][6][7][8][9][10][11][12] Paper is indeed an advantageous material for sensor fabrication can wrap around the cellulose fibers and change their interfacial chemistry. For instance, negatively charged cellulose can be rendered positively charged when modified with a polymer bearing amines.…”

Section: Introductionmentioning

confidence: 99%

Polymer Components for Paper‐Based Analytical Devices

Campo

Vaquer

Rica

2022

Adv Materials Technologies

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In recent years, paper has become an essential substrate material for developing different types of sensors, from wearable devices to single‐use test strips and biosensors. In parallel, a polymer‐based toolbox has emerged in order to add additional functions to these paper‐based analytical devices. In this article, examples are compiled from the recent literature showing microfluidic systems based on printing impermeable polymer barriers with different methods as well as the implementation of electrochemical, fluorescent, and colorimetric polymer‐based transducers in paper‐based analytical platforms. Externally actuated or dissolvable polymer valves and reservoirs, analyte concentrators, and separation devices, as well as polymer‐based recognition elements (molecularly imprinted polymers) printed on paper substrates are also reviewed. The search has revealed a plethora of possibilities for developing complex lab‐on‐chip devices implementing different polymer‐based components in them. The ability of patterning these polymers with common printing methods that do not require specialized facilities paves the way for mass producing this kind of advanced paper‐based analytical device.

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Point-of-care diagnostics for rapid determination of prostate cancer biomarker sarcosine: application of disposable potentiometric sensor based on oxide-conductive polymer nanocomposite

et al. 2023

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One of the most important reasons for an increased mortality rate of cancer is late diagnosis. Point-of-care (POC) diagnostic sensors can provide rapid and cost-effective diagnosis and monitoring of cancer biomarkers. Portable, disposable, and sensitive sarcosine solid-contact ion-selective potentiometric sensors (SC-ISEs) were fabricated as POC analyzers for the rapid determination of the prostate cancer biomarker sarcosine. Tungsten trioxide nanoparticles (WO3 NPs), polyaniline nanoparticles (PANI NPs), and PANI-WO3 nanocomposite were used as ion-to-electron transducers on screen-printed sensors. WO3 NPs and PANI-WO3 nanocomposite have not been investigated before as ion-to-electron transducer layers in potentiometric SC sensors. The designated sensors were characterized using SEM, XRD, FTIR, UV-VIS spectroscopy, and EIS. The inclusion of WO3 and PANI in SC sensors enhanced the transduction at the interface between the screen-printed SC and the ion-selective membrane, offering lower potential drift, a longer lifetime, shorter response time, and better sensitivity. The proposed sarcosine sensors exhibited Nernstian slopes over linear response ranges 10−3–10−7 M, 10−3–10−8 M, 10−5–10−9 M, and 10−7–10−12 M for control, WO3 NPs, PANI NPs, and PANI-WO3 nanocomposite-based sensors, respectively. From a comparative point of view between the four sensors, PANI-WO3 nanocomposite inclusion offered the lowest potential drift (0.5 mV h−1), the longest lifetime (4 months), and the best LOD (9.95 × 10−13 M). The proposed sensors were successfully applied to determine sarcosine as a potential prostate cancer biomarker in urine without prior sample treatment steps. The WHO ASSURED criteria for point-of-care diagnostics are met by the proposed sensors. Graphical abstract

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Colorimetric paper-based sarcosine assay with improved sensitivity

Cited by 21 publications

References 46 publications

Highly Sensitive Photothermal Microfluidic Thread-Based Duplex Immunosensor for Point-of-Care Monitoring

Highly Sensitive Photothermal Microfluidic Thread-Based Duplex Immunosensor for Point-of-Care Monitoring

Polymer Components for Paper‐Based Analytical Devices

Point-of-care diagnostics for rapid determination of prostate cancer biomarker sarcosine: application of disposable potentiometric sensor based on oxide-conductive polymer nanocomposite

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