Colorimetric sensing of glucose and GSH using core–shell Cu/Au nanoparticles with peroxidase mimicking activity

Sun, Ruimeng; Lv, Ruijuan; Zhang, Yang; Du, Ting; Li, Yuhan; Chen, Lixia

doi:10.1039/d2ra02375j

Cited by 21 publications

(10 citation statements)

References 56 publications

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“…Therefore, a new glucose biosensor has been developed by combining the high specificity of GOx with a remarkable a-RuS S8, Supporting Information). [6,76,77] In addition, the interference-resistant of a-RuS 2 has been revealed by using interfering compounds including glucose analogs, ions, and glutathione (Figure 7f), and the results demonstrate that the detection system has great glucose selectivity. In addition, to evaluate the practicality of a-RuS 2 in glucose detection, recovery experiments is performed in fetal bovine serum.…”

Section: Resultsmentioning

confidence: 95%

“…In addition, a good linear relationship appears between absorbance and glucose concentration (0–800 × 10 −6 m ), and the LOD is 9.84 × 10 −6 m , lower than that of recently reported enzymatic biodetection systems (Figure 7d,e; Table S8, Supporting Information). [ 6,76,77 ] In addition, the interference‐resistant of a‐RuS 2 has been revealed by using interfering compounds including glucose analogs, ions, and glutathione (Figure 7f), and the results demonstrate that the detection system has great glucose selectivity. In addition, to evaluate the practicality of a‐RuS 2 in glucose detection, recovery experiments is performed in fetal bovine serum.…”

Section: Resultsmentioning

confidence: 98%

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Amorphization‐Modulated Metal Sulfides with Boosted Active Sites and Kinetics for Efficient Enzymatic Colorimetric Biodetection

Deng

Xing

et al. 2023

Small Methods

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Colorimetric biosensing has become a popular sensing method for the portable detection of a variety of biomarkers. Artificial biocatalysts can replace traditional natural enzymes in the fields of enzymatic colorimetric biodetection; however, the exploration of new biocatalysts with efficient, stable, and specific biosensing reactions has remained challenging so far. Here, to enhance the active sites and overcome the sluggish kinetics of metal sulfides, the creation of an amorphous RuS 2 (a-RuS 2 ) biocatalytic system is reported, which can dramatically boost the peroxidase-mimetic activity of RuS 2 for the enzymatic detection of diverse biomolecules. Due to the existence of abundant accessible active sites and mildly surface oxidation, the a-RuS 2 biocatalyst displays a twofold V max value and much higher reaction kinetics/turnover number (1.63 × 10 −2 s −1 ) compared to that of the crystallized RuS 2 . Noticeably, the a-RuS 2 -based biosensor shows an extremely low detection limit of H 2 O 2 (3.25 × 10 −6 m), l-cysteine (3.39 × 10 −6 m), and glucose (9.84 × 10 −6 m), respectively, thus showing superior detection sensitivity to many currently reported peroxidase-mimetic nanomaterials. This work offers a new path to create highly sensitive and specific colorimetric biosensors in detecting biomolecules and also provides valuable insights for engineering robust enzyme-like biocatalysts via amorphization-modulated design.

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Section: Resultsmentioning

confidence: 95%

Section: Resultsmentioning

confidence: 98%

Amorphization‐Modulated Metal Sulfides with Boosted Active Sites and Kinetics for Efficient Enzymatic Colorimetric Biodetection

Deng

Xing

et al. 2023

Small Methods

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“…As seen in Table , colorimetric glucose biosensor studies based on different strategies have been carried out. The most commonly used among them is the colorimetric sensor based on the formation and measurement of the absorbance of TMB with different nanomaterials showing peroxidase-like properties. − Compared to these methods, the LOD value of the novel CUPRAC–GDH-based biosensor was generally found to be close to or even lower than the values reported in some of these studies. It is seen that the LOD value of the GDH–CUPRAC-based biosensor was also found to be lower than those of biosensors based on ABTS, , I 2 -starch, o -dianisidine, FeSCN 2+ including Fenton reaction, and CUPRAC reagents, which is quite similar to our study but using the GOx enzyme.…”

Section: Resultsmentioning

confidence: 96%

Fabrication of a Novel Optical Glucose Biosensor Using Copper(II) Neocuproine as a Chromogenic Oxidant and Glucose Dehydrogenase-Immobilized Magnetite Nanoparticles

Ayaz,

Üzer,

Dilgin

et al. 2023

ACS Omega

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This study describes a novel optical glucose biosensor based on a colorimetric reaction between reduced nicotinamide adenine dinucleotide (NADH) and a copper(II) neocuproine complex ([Cu(Nc) 2 ] 2+ ) as a chromogenic oxidant. An enzymatic reaction takes place between glucose and glucose dehydrogenase (GDH)–chitosan (CS) immobilized on silanized magnetite nanoparticles (CS@SiO 2 @Fe 3 O 4 ) in the presence of coenzyme NAD + . The oxidation of glucose to gluconolactone via the immobilized enzyme is coupled with the reduction of NAD + to NADH at the same time. After the separation of GDH-immobilized SiO 2 @Fe 3 O 4 with a magnet, the enzymatically produced NADH chemically reduces the chromogenic oxidant cupric neocuproine to the cuprous chelate. Thus, the glucose biosensor is fabricated based on the measurement of the absorbance of the formed yellow-orange complex ([Cu(Nc) 2 ] + ) at 450 nm. The obtained results show that the colorimetric biosensor has a wide linear response range for glucose, between 1.0 and 150.0 μM under optimized conditions. The limit of detection and limit of quantification were found to be 0.31 and 1.02 μM, respectively. The selectivity properties of the fabricated biosensor were tested with various interfering species. This biosensor was applied to various samples, and the obtained results suggest that the fabricated optical biosensor can be successfully used for the selective and sensitive determination of glucose in real samples.

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“…For example, Sun et al established core-shell Cu/Au nanoparticles (Cu/Au NPs) as a nanozyme for the detection of the GSH with the linear range of of 0.07 to 1.43 mM. [46] The reported LOD of GSH using Cu/Au NPs is 13 μM which is suitable for the sensing application for GSH detection.…”

Section: Introductionmentioning

confidence: 99%

“…To the best of our awareness, there are only a few reports on GSH detection utilizing bimetallic NPs incorporated on reduced graphene oxide sheets in an aqueous medium. For example, Sun et al established core–shell Cu/Au nanoparticles (Cu/Au NPs) as a nanozyme for the detection of the GSH with the linear range of of 0.07 to 1.43 mM [46] . The reported LOD of GSH using Cu/Au NPs is 13 μM which is suitable for the sensing application for GSH detection.…”

Section: Introductionmentioning

confidence: 99%

A Facile Preparation of Reduced Graphene Oxide Capped AuAg Bimetallic Nanoparticles: A Selective Nanozyme for Glutathione Detection

et al. 2022

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Over the last decade, artificial enzymes (nanozymes) have undergone substantial development in the field of sensing. In this study, Au−Ag bimetallic nanoparticles (NPs) decorated on reduced graphene oxide sheets (AuAg‐rGO) nanocomposite has been developed as a novel colorimetric probe based on the peroxidase imitating activity of the nanozyme. AuAg‐rGO nanocomposite proved to feature an intrinsic peroxidase mimic activity, facilitating the oxidation of a peroxidase substrate like 3,3’,5,5’‐tetramethylbenzidine (TMB) with high efficiency to oxidised TMB (blue coloured product) with the aid of hydrogen peroxide (H2O2). The characteristic Michaelis‐Menten kinetics study allowed the establishment of the catalytic performance of AuAg‐rGO as an artificial enzyme. Glutathione (GSH) displays inhibiting effect on the oxidation of the peroxidase substrate, TMB. Therefore, AuAg‐rGO nanozyme was applied for detection of GSH and exhibited exceptional selectivity and sensitivity with a low limit of detection (LOD) of 38 nM within the 0–200 μM concentration range. The artificial nanozyme system reported here is simple, convenient, and straightforward for colorimetric sensing of GSH.

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Colorimetric sensing of glucose and GSH using core–shell Cu/Au nanoparticles with peroxidase mimicking activity

Abstract: Core–shell Cu/Au nanoparticles were synthesized by a one pot method, their peroxidase activity was proved by catalysing the oxidation of 3,3′,5,5′-tetramethylbenzidine with colour change to blue. Results showed a good range and limit for the detection of glucose and GSH.

Cited by 21 publications

References 56 publications

Amorphization‐Modulated Metal Sulfides with Boosted Active Sites and Kinetics for Efficient Enzymatic Colorimetric Biodetection

Amorphization‐Modulated Metal Sulfides with Boosted Active Sites and Kinetics for Efficient Enzymatic Colorimetric Biodetection

Fabrication of a Novel Optical Glucose Biosensor Using Copper(II) Neocuproine as a Chromogenic Oxidant and Glucose Dehydrogenase-Immobilized Magnetite Nanoparticles

A Facile Preparation of Reduced Graphene Oxide Capped AuAg Bimetallic Nanoparticles: A Selective Nanozyme for Glutathione Detection

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